Search results for "Fluorescence"

Techniques d'études de la formation des biofilms. Retour d'expérience issu du domaine "agro-alimentaire" et du domaine "environnemental"

2008

No abstract

Coumarin-Phosphine-Based Smart Probes for Tracking Biologically Relevant Metal Complexes: From Theoretical to Biological Investigations

2016

International audience; Ten metal-based complexes and associated ligands have been synthesized and characterized. One of the metal ligands is a coumarin-phosphine derivative, which displays tunable fluorescence properties. The fluorescence is quenched in the case of the free ligand and ruthenium and osmium complexes, whereas it is strong for the gold complexes and phosphonium derivatives. These trends were rationalized by theoretical calculations, which revealed non-radiative channels involving a dark state for the free ligands that is lower in energy than the emissive state and is responsible for the quenching of fluorescence. For the Ru-II and Os-II complexes, other non-radiative channels…

Fluidity and cytosolic Ca2+ concentration of circulating polymorphonuclear leukocytes at baseline in some chronic and acute clinical conditions: revi…

2016

Abstract. Objective: In this mini-review we describe the behavior of polymorphonuclear leukocyte (PMN) membrane fluidity and of PMN cytosolic Ca2+ concentration in some chronic and acute clinical conditions. Methods: PMN membrane fluidity was evaluated employing the fluorescent probe Fura-2AM, and PMN cytosolic Ca2+ concentration was evaluated using the fluorescent probe TMA-DPH. Results: From the determination of these two parameters investigated on resting PMNs, an almost constant increase in PMN cytosolic Ca2+ concentration in chronic clinical conditions, such as vascular atherosclerotic disease with and without diabetes mellitus, essential hypertension, chronic kidney disease, and diabe…

A novel homologous model for noninvasive monitoring of endometriosis progression.

2017

To date, several groups have generated homologous models of endometriosis through the implantation of endometrial tissue fluorescently labeled by green fluorescent protein (GFP) or tissue from luciferase-expressing transgenic mice into recipient animals, enabling noninvasive monitoring of lesion signal. These models present an advantage over endpoint models, but some limitations persist; use of transgenic mice is laborious and expensive, and GFP presents poor tissue penetration due to the relatively short emission wavelength. For this reason, a homologous mouse model of endometriosis that allows in vivo monitoring of generated lesions over time and mimics human lesions in recipient mice wou…

Alkylated Aromatic Thioethers with Aggregation‐Induced Emission Properties—Assembly and Photophysics

2019

In this contribution, we present the synthesis and self-assembly of alkylated thioethers with interesting photophysical properties. To this end, the emission, absorption and excitation spectra in organic solvents and as aggregates in water were measured as well as the corresponding photoluminescence quantum yields and lifetimes. The aggregates in aqueous media were visualized and measured using transmission electron microscopy. Besides that, crystal structures of selected compounds allowed a detailed discussion of the structure–property relationship. Furthermore, the mesomorphic behavior was investigated using polarized optical microscopy (POM) as well as differential scanning calorimetry (…

Synthesis of fluorescent (benzyloxycarbonylamino)(aryl)methylphosphonates

2014

The synthesis of a library of structurally variable aromatic esters of (benzyloxycarbonylamino)(aryl)methylphosphonic acids is described by means of the Oleksyszyn reaction. The library was enlarged by the application of a Suzuki–Miayra approach and by preparation of mixed esters.

Molecular Insights on alpha-synuclein mediated membrane disruption: protein-lipid co-aggregates formation

2014

Sarkana āboliņa, Moldāvijas pūķgalves un šaurlapu ugunspuķes drogu kvalitātes kontrole izmantojot konfokālo lāzerskenējošo mikroskopu

2020

Pētījuma mērķis: Novērtēt drogu sagatavošanas un uzglabāšanas kvalitātes atbilstību Pasaules Veselības organizācijas vadlīnijām un pārbaudīt izveidoto mikroskopisko metožu universalitāti. Pētījuma metodes: Tēju drogās novērtēja šūnu dzīvotspēju, morfoloģiju, hloroplastu hlorofila fluorescenci un mikrobioloģisko piesārņojumu. Nozīmīgākie rezultāti un secinājumi: Neatbilstošos apstākļos ievāktas un uzglabātas, ka arī neatbilstošā temperatūrā dehidratētas drogās rodas kvalitātes īpašību un bioloģiski aktīvo vielu zudumi. Lai novērtētu drogu kvalitāti ir izveidots šūnu bioloģijas un mikroskopisko metožu kopums. Tas ļāvis novērtēt saimniecības ražošanas procesā sagatavoto drogu kvalitāti. Turpmā…

Improved method to retain cytosolic reporter protein fluorescence while staining for nuclear proteins

2014

Staining of transcription factors (TFs) together with retention of fluorescent reporter proteins is hindered by loss of fluorescence using current available methods. In this study, it is shown that current TF staining protocols do not destroy fluorescent proteins (FPs) but rather that fixation is not sufficient to retain FPs in the cytosol of the permeabilized cells. In this article, a simple and reliable protocol is elaborated, which allows efficient TF and cytokine staining while retaining FPs inside fixed cells.

Identification and quantification of microbial populations in activated sludge and anaerobic digestion processes

2014

Eight different phenotypes were studied in an activated sludge process (AeR) and anaerobic digester AnD) in a full-scale wastewater treatment plant by means of fluorescent in situ hybridization (FISH) and automated FISH quantification software. The phenotypes were ammonia-oxidizing bacteria, nitrite-oxidizing bacteria, denitrifying bacteria, phosphate-accumulating organisms (PAO), glycogen-accumulating organisms (GAO), sulphate-reducing bacteria (SRB), methanotrophic bacteria and methanogenic archaea. Some findings were unexpected: (a) Presence of PAO, GAO and denitrifiers in the AeR possibly due to unexpected environmental conditions caused by oxygen deficiencies or its ability to survive …