Search results for "Frame"

Annotation of microsporidian genomes using transcriptional signals

2012

EA GenoSol CT3; International audience; High-quality annotation of microsporidian genomes is essential for understanding the biological processes that govern the development of these parasites. Here we present an improved structural annotation method using transcriptional DNA signals. We apply this method to re-annotate four previously annotated genomes, which allow us to detect annotation errors and identify a significant number of unpredicted genes. We then annotate the newly sequenced genome of Anncaliia algerae. A comparative genomic analysis of A. algerae permits the identification of not only microsporidian core genes, but also potentially highly expressed genes encoding membrane-asso…

Humour as a symptom of research trends in translation studies

2017

This article is an overview of translation studies applied to the case of humour, divided into four parts, plus an extensive bibliography. The first part goes over humour translation as a relevant object of research and why it is worthy of more academic attention. Humour translation should not be dealt with or looked upon as a strange body within translation studies. Part two is an overview of key contributions to the field, from Spain and elsewhere, covering a considerable number of authors and theories. Part three focuses on promising areas of interest for researchers and illustrates how audiovisual translation is a good instance of dynamism within the field, connecting all this to the ri…

An explanation for unexpected 802.11 outdoor link-level measurement results

2008

This paper provides experimental evidence that "weird'/poor outdoor link-level performance measurements may be caused by driver/card-specific antenna diversity algorithms unexpectedly supported/activated at the WLAN transmitter side. We focus our analysis on the Atheros/MADWiFi card/driver case, and we observe that the transmit antenna diversity mechanisms remain by default enabled when the available antennas are not homogeneous in terms of gain or, even worse, when only a single antenna is connected. This may cause considerable performance impairments (large frame loss ratio), in conditions frequently encountered in outdoor link deployments. The negative impact of transmit antenna diversit…

Structural and evolutionary analysis of the copia-like elements in the Arabidopsis thaliana genome.

2001

The analysis of 460 kb of genomic sequence of Arabidopsis thaliana chromosome III allowed us to identify two new transposable elements named AtC1 and AtC2. AtC1 shows identical long terminal repeats (LTRs) and all the structural features characteristic of the copia-like active elements. AtC2 is also a full copia-like element, but a putative stop codon in the open reading frame (ORF) would produce a truncated protein. In order to identify the copia-like fraction of the A. thaliana genome, a careful computer-based analysis of the available sequences (which correspond to 92% of the genome) was performed. Approximately 300 nonredundant copia-like sequences homologous to AtC1 and AtC2 were detec…

Tirant is a new member of the gypsy family of retrotransposons in Drosophila melanogaster.

2000

In this paper, we propose a consensus sequence for a putative complete Tirant retrotransposon. Several defective copies, as well as relevant sequences available in databases have been analyzed. The putative complete Tirant element is 8533 bp long, and presents all the structural features of a retroviruslike transposable element of the gypsy family. It contains three ORFs (open reading frames) that encode putative products resembling the retroviral Gag, Pol, and Env proteins. Southern blot analyses show that complete and defective Tirant elements are widespread in Drosophila melanogaster. The different hybridization patterns observed in several natural populations of this species suggest tha…

bilbo, a non-LTR retrotransposon of Drosophila subobscura: a clue to the evolution of LINE-like elements in Drosophila

1997

We used the repetitive character of transposable elements to isolate a non-LTR retrotransposon in Drosophila subobscura. bilbo, as we have called it, has homology to TRIM and LOA elements. Sequence analysis showed a 5' untranslated region (UTR), an open reading frame (ORF) with no RNA-binding domains, a downstream ORF that had structural homology to that of the I factor, and, finally, a 3' UTR which ended in several 5-nt repeats. The results of our phylogenetic and structural analyses shed light on the evolution of Drosophila non-LTR retrotransposons and support the hypothesis that an ancestor of these elements was structurally complex.

Drosophila melanogaster histone H2B retropseudogene is inserted into a region rich in transposable elements.

1998

We have isolated and characterized the genomic sequence of a Drosophila melanogaster histone H2B pseudogene that is localized outside of the cluster of the replication-dependent histone genes and has all the properties of a retropseudogene. It is highly homologous to the transcribed region of the D. melanogaster histone H2B gene, but not to its flanking regions, and is surrounded by short direct repeats. The pseudogene contains several point mutations that preclude its translation. The sequence of the 3' region of this pseudogene is compatible with the hypothesis that the 3' terminal stem-loop structure of the histone H2B mRNA has served as a primer for the reverse transcription event from …

A new design approach to the use of composite materials for heavy transport vehicles

2007

In order to keep or to reach a high level of competitiveness and performance of a product, it is necessary to explore all the possible solutions that allow the best compromise between costs and project requirements. By this point of view the study of alternative designs and/or materials to use, is an important aspect that can identify a new concept or way of thinking about a product. This paper presents how to make use of composite materials in the field of heavy vehicles transportation. A new semitrailer in composite material has been designed, using a methodical redesign approach and an optimisation process. The main innovation in this project is, besides the use of the Glass Fibre Reinfo…

2019

Impact is increasingly important for science policy-makers; science policy studies has reacted this heightened urgency by studying these policy-interventions meaning that policy has developed more quickly than theory. This has led to the prevalence of a ‘common sense’ impact definition: research’s societal impact are direct economic effects, such as income generated by licenses, patents and spin-out companies. These indicators are recognised as weak proxies for research’s societal benefits, and in response, science policy has undertaken a huge descriptive effort to more precisely define impact. SSH disciplines have been highly active in this because economic metrics are very poor measures o…

Influence of geographical extent on typology- and model-based assessments of taxonomic completeness of river macroinvertebrates

2009

Summary 1. River InVertebrate Prediction and Classification System (RIVPACS)-type predictive models are increasingly used to assess the biological condition of freshwaters, but management schemes may also be based on a priori groupings of similar water bodies (typologies) to control for natural variation in biota. The two approaches may lead to disagreements of the biological status of a site, depending on, for example, the spatial scale at which assessments are conducted. 2. We used data from 96 reference and 134 potentially impacted sites from Western and Central Finland to compare RIVPACS-type models and a simple size-based typology of rivers for the assessment of taxonomic completeness …