Search results for "General Veterinary"

Recombinant virus-like particles as a carrier of B- and T-cell epitopes of hepatitis C virus (HCV)

2005

The major aim of the project was the development of virus-like particles (VLP) displaying B- and T-cell epitopes of hepatitis C virus (HCV) proteins. To this end, hepatitis B virus core (HBc) particles were used as a carrier of HCV epitopes. Fragments of HCV genes encoding core (aa 98) and NS3 (aa 155) proteins were fused to the 3' terminus of the truncated HBV core gene. All recombinant plasmids led to relatively high levels of expression of chimeric proteins in E. coli, which resulted in the formation of complete "mature" VLP. Chimeric HBc/HCV VLPs were purified by combination of gel filtration and sucrose gradient centrifugation, and used for immunogenicity studies in mice. All variants …

Nasal immunization of mice with a rotavirus DNA vaccine that induces protective intestinal IgA antibodies

2004

International audience; DNA vaccination using a plasmid encoding the rotavirus inner capsid VP6 has been explored in the mouse model of rotavirus infection. BALB/c mice were immunized with a VP6 DNA vaccine by the intramuscular, nasal and oral routes. VP6 DNA vaccination by the nasal and oral routes induced the production of anti-VP6 IgA antibodies by intestinal lymphoid cells. Intramuscular DNA injection stimulated the production of serum anti-VP6 IgG but not serum anti-VP6 IgA antibodies. Protection against shedding of rotaviruses in stools after oral challenge with the murine EDIM rotavirus strain was investigated in the immunized mice. A significant reduction in the level of rotavirus a…

COVID-19 mRNA BNT162b2 vaccine safety and B-cell and T-cell reactogenicity among children with a history of paediatric multisystem inflammatory syndr…

2023

Pilzbefall m�nnlicher Puparien von Xenos vesparum Rossi, 1794 (Strepsiptera, Insecta)

1968

Es wird erstmals uber Pilzbefall in verschiedenen mitteleuropaischen Populationen des auf Polistes gallicus L. (Hymenoptera) schmarotzenden Xenos vesparumRossi (Insecta, Strepsiptera) berichtet. Im Gegensatz zu fruheren Beobachtungen an Heteropteren-Parasiten ist wahrscheinlich, das Penicillium sp. die Strepsipteren nicht nur als Saprophyt, sondern bereits als Wundparasit befallt. Ein Weiterwachsen des Pilzes in das Wirtsinsekt oder dessen Befall durch Pilze uber verlassene Strepsipteren-Puparien ist wenigstens bei den Hymenopterenparasiten nicht die Regel.

First helminthological data on the Iberian adder, Vipera seoanei.

2023

Abstract Helminth fauna and some biotic and abiotic traits were analysed in Iberian adder Vipera seoanei (Squamata: Viperidae) a European viper nearly endemic to northern Iberian Peninsula, in an attempt to confirm previous claims of relationships between this ecological traits in this genus. A total of 317 samples mostly covering the distributional range of V. seoanei, were examined. Compared with other Vipera species from the Iberian Peninsula, the helminth fauna is also impoverished in V. seoanei, but it is contrary to other Vipera species from Central and East Europe. Helminths were mostly found in adult vipers, and occurred in vipers located at the periphery of the species range, chara…

Protection of rabbits against rabbit viral haemorrhagic disease with a vaccinia-RHDV recombinant virus

1996

In order to protect domestic and wild rabbits against RVHD, we constructed a recombinant vaccinia-RHDV virus, using the Copenhagen strain of the vaccinia virus. This recombinant virus expressed the RHDV capsid protein (VP60). Analysis of the expressed product showed that the recombinant protein, which is 60 kDa in size, was antigenic as revealed by its reactions in immunoprecipitation and indirect immunofluorescence with the antibodies raised against RHDV. The recombinant virus induced high level of RHDV specific antibodies in rabbits following immunization. Inoculations by both the intradermal and oral routes allow protection of animals against a challenge with virulent RHDV.

Tear production and intraocular pressure values in clinically normal eyes of whooper swans (<i>Cygnus cygnus</i>)

2018

The objective of this study was to determine the normal range of tear production and values of intraocular pressure (IOP) in clinically normal eyes of whooper swans (Cygnus cygnus). A total of 86 birds: 81 cygnets (6-10 weeks old) and 5 moulting - adult swans (3-6 years old) were examined in this study. Both eyes of all birds underwent a complete ophthalmic examination, including evaluation of tear production with the phenol red thread test (PRTT) and the assessment of the IOP by rebound tonometry, employing the TonoVet® device. The mean ± standard deviation of PRTT values were as follows: all cygnets (81) 22.59±3.48 mm/15s, male cygnets (45) 22.64±3.54 mm/15s, female cygnets (36) 22.53±3.4…

18S rRNA gene sequences and phylogenetic relationships of European hard-tick species (Acari: Ixodidae)

1998

The complete 18S rRNA gene sequences of the following six European hard-tick species were obtained by direct PCR cycle sequencing and silver-staining methods: Rhipicephalus pusillus, Boophilus annulatus, Dermacentor marginatus, Hyalomma lusitanicum, Haemaphysalis punctata, and Ixodes ricinus. Differences observed in the sequence alignment of these six species together with the 18S rRNA gene sequences of 13 other hard-tick species demonstrate that this gene is a good marker for supraspecific differentiation as well as genus grouping among hard ticks. Phylogenetic analyses strongly support that Hyalomma species share a common ancestor with Rhipicephalinae and, consequently, Hyalomminae should…

Phylogeny and prevalence of kobuviruses in dogs and cats in the UK

2013

AbstractThe kobuviruses represent an emerging genus in the Picornaviridae. Here we have used next generation sequencing and conventional approaches to identify the first canine kobuvirus (CaKoV) from outside the USA. Phylogenetic analysis suggests that a single lineage genotype of CaKoV now exists in Europe and the USA with 94% nucleotide similarity in the coding region. CaKoV was only identified in a single case from a case–control study of canine diarrhoea, suggesting this virus was not a frequent cause of disease in this population. Attempts to grow CaKoV in cell culture failed. Sequence analysis suggested CaKoV was distinct from human Aichi virus (AiV), and unlikely to pose a significan…

Influence of the Fermented Feed and Vaccination and Their Interaction on Parameters of Large White/Norwegian Landrace Piglets

2020

The aim of this study was to evaluate the influence of fermented with a newly isolated lactic acid bacteria (LAB) strains combination (Lactobacillus plantarum LUHS122, Lactobacillus casei LUHS210, Lactobacillus farraginis LUHS206, Pediococcus acidilactici LUHS29, Lactobacillus plantarum LUHS135 and Lactobacillus uvarum LUHS245) feed on non-vaccinated (NV) and vaccinated with Circovac porcine circovirus type 2 vaccine (QI09AA07, CEVA-PHYLAXIA Co. Ltd. Sz&aacute