Search results for "HeLa"

showing 10 items of 738 documents

Synthesis and biological evaluation of (+)-labdadienedial, derivatives and precursors from (+)-sclareolide

2010

Labdadienedial and a series of C15,C16-functionalized derivatives were synthesized from commercial (+)-sclareolide and evaluated for their cytotoxic, antimycotic, and antiviral activities. Their precursors were similarly evaluated.

Antifungal AgentsStereochemistryAntineoplastic AgentsHerpesvirus 1 HumanAntiviral AgentsChemical synthesisInhibitory Concentration 50chemistry.chemical_compoundChlorocebus aethiopsDrug Discoveryotorhinolaryngologic diseasesAnimalsHumansCytotoxicityVero CellsPharmacologyOrganic ChemistryFungifood and beveragesSclareolideBiological activityGeneral MedicineCombinatorial chemistryTerpenoidIn vitrostomatognathic diseaseschemistrylipids (amino acids peptides and proteins)DiterpenesDiterpeneEnantiomerHeLa CellsEuropean Journal of Medicinal Chemistry
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Synthesis and biological evaluation of pyridinebetaine A and B

2009

The synthesis of the marine natural products pyridinebetaine A and B is reported. The biological evaluation of pyridinebetaine A and B and several analogues as cytotoxic, antifungal and antiviral agents is also described. Unfortunately, none of the compounds tested showed relevant antifungal or cytotoxic activity. Only pyridinebetaine B reduced the Herpes simplex virus type 1 virus replication, though only weakly.

AntifungalAntifungal Agentsmedicine.drug_classHerpesvirus 1 HumanPlant ScienceBiologyVirus Replicationmedicine.disease_causeAntiviral AgentsBiochemistryAnalytical ChemistryMicrobiologyStructure-Activity RelationshipAlkaloidsChlorocebus aethiopsmedicineAnimalsHumansCytotoxic T cellVero CellsBiological evaluationMolecular StructureOrganic ChemistryFungiVirologyBetaineHerpes simplex virusViral replicationHeLa CellsNatural Product Research
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Synthesis, antimony-121 mössbauer and infrared spectral characterization of antimony trifluoride and catecholatoantimony(III) fluoride adducts with t…

1988

Abstract The synthesis of the adducts SbF 3 (L) and (C 6 H 4 O 2 )SbF(L) [L = pyridine-2-carboxaldehyde- 2-pyridylhydrazone (paphyH); 2,2′:6′,2″-terpyridine (terpy) and 2,4,6-tris(2-pyridyl)-1,3,5-triazine (tpta)] is reported. Infrared data are consistent with the occurred chelation from the terdentate ligand providing an overall pseudo-seven coordination at Sb. The stereoactivity of the antimony lone pair is inferred from the large value of the Mossbauer quadrupole coupling constant observed for all the compounds.

Antimony trifluorideChemistryLigandInorganic chemistrychemistry.chemical_elementAdductInorganic Chemistrychemistry.chemical_compoundCrystallographyAntimonyMössbauer spectroscopyMaterials ChemistryChelationPhysical and Theoretical ChemistryLone pairFluorideInorganica Chimica Acta
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Toward a Rational Design of Polyamine-Based Zinc-Chelating Agents for Cancer Therapies.

2020

In vitro viability assays against a representative panel of human cancer cell lines revealed that polyamines L1a and L5a displayed remarkable activity with IC50 values in the micromolar range. Preliminary research indicated that both compounds promoted G1 cell cycle arrest followed by cellular senescence and apoptosis. The induction of apoptotic cell death involved loss of mitochondrial outer membrane permeability and activation of caspases 3/7. Interestingly, L1a and L5a failed to activate cellular DNA damage response. The high intracellular zinc-chelating capacity of both compounds, deduced from the metal-specific Zinquin assay and ZnL2+ stability constant values in solution, strongly sup…

Antineoplastic AgentsApoptosis01 natural sciences03 medical and health scienceschemistry.chemical_compoundStructure-Activity RelationshipCell Line TumorDrug DiscoveryPolyaminesHumansCytotoxicityCaspase030304 developmental biologyChelating Agents0303 health sciencesbiologyMolecular StructureChemistryRational designG1 Phase Cell Cycle Checkpoints0104 chemical sciencesCell biology010404 medicinal & biomolecular chemistryZincModels ChemicalApoptosisCell cultureDrug Designbiology.proteinMolecular MedicineQuantum TheoryDrug Screening Assays AntitumorPolyamineG1 phaseIntracellularJournal of medicinal chemistry
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Preparation of C-23 esterified silybin derivatives and evaluation of their lipid peroxidation inhibitory and DNA protective properties.

2009

A diverse series of C-23 esterified silybin derivatives (1a-n) were designed and synthesized. The antioxidative properties of these compounds were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide anion radical scavenging, ferrous ion chelation, and inhibition of rat liver homogenate lipid peroxidation. Their protective effects on the prevention of hydrogen peroxide induced DNA damage were also investigated. Most of the synthesized compounds exhibited more effective antioxidant activities than silybin. The esterified silybin analogues displayed satisfactory performance especially on iron chelation and antiperoxidative activity. Compound 1n in particular exhibited remarkable a…

AntioxidantDNA damageDPPHmedicine.medical_treatmentClinical BiochemistryPharmaceutical ScienceSilibininBiochemistryLipid peroxidationchemistry.chemical_compoundStructure-Activity RelationshipDrug DiscoverymedicineAnimalsChelationMolecular BiologyChemistryOrganic ChemistryFree Radical ScavengersFree radical scavengerRatsBiochemistrySilybinMolecular MedicineLipid PeroxidationQuercetinNuclear chemistryDNA DamageSilymarinBioorganicmedicinal chemistry
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Antioxidant and neuroprotective effects of synthesized sintenin derivatives

2009

Three series of sintenin derivatives (compounds 1-14) were designed and prepared and their antioxidative and neuroprotective effects were evaluated. The in vitro models of scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, chelating ferrous ions, inhibiting the rat brain homogenates lipid peroxidation, and protecting neurons damaged by hydrogen peroxide were employed for bioassays. It was found that sintenin derivatives 4 and 13 showed remarkable antioxidative and neuroprotective activities.

AntioxidantDPPHmedicine.medical_treatmentRadicalNeuroprotectionAntioxidantsRats Sprague-DawleyLipid peroxidationchemistry.chemical_compoundPicratesDrug DiscoverymedicineAnimalsChelationHydrogen peroxideCells CulturedChelating AgentsNeuronsPharmacologyChemistryBiphenyl CompoundsHydrogen PeroxideGeneral MedicineRatsBiphenyl compoundNeuroprotective AgentsBiochemistryLipid PeroxidationPropionatesJournal of Enzyme Inhibition and Medicinal Chemistry
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The symbiosis between Nicotiana tabacum and the endomycorrhizal fungus Funneliformis mosseae increases the plant glutathione level and decreases leaf…

2015

Over time, anthropogenic activities have led to severe cadmium (Cd) and arsenic (As) pollution in several environments. Plants inhabiting metal(loid)-contaminated areas should be able to sequester and detoxify these toxic elements as soon as they enter roots and leaves. We postulated here that an important role in protecting plants from excessive metal(loid) accumulation and toxicity might be played by arbuscular mycorrhizal (AM) fungi. In fact, human exploitation of plant material derived from Cd- and As-polluted environments may lead to a noxious intake of these toxic elements; in particular, a possible source of Cd and As for humans is given by cigarette and cigar smoke. We investigated …

AntioxidantPhysiologyNicotiana tabacummedicine.medical_treatmentAM fungi Arsenic Cadmium Cigarettes Glutathione Mycorrhiza Smoking Tobacco ARBUSCULAR MYCORRHIZAL FUNGI PHOSPHATE-UPTAKE SYSTEM HOLCUS-LANATUS L GLOMUS-MOSSEAE PHYTOCHELATIN SYNTHASE TRANSGENIC TOBACCO BINDING PEPTIDES L. TYPES ACCUMULATION TOLERANCEPlant SciencePlant RootsAntioxidantsARBUSCULAR MYCORRHIZAL FUNGIGlomeromycotachemistry.chemical_compoundMycorrhizaeSoil PollutantsMycorrhizaHOLCUS-LANATUS LCadmiumbiologyMedicine (all)SmokingAdaptation PhysiologicalGlutathioneAM fungi; Arsenic; Cadmium; Cigarettes; Glutathione; Mycorrhiza; Smoking; Tobacco; Adaptation; Physiological; Antioxidants; Glomeromycota; Mycorrhizae; Plant Leaves; Plant Roots; Soil Pollutants; Stress; Symbiosis; Plant Science; Genetics; Physiology; Medicine (all)AM fungiBINDING PEPTIDESCadmiumSettore BIO/07 - EcologiaGLOMUS-MOSSEAEPhysiologicalchemistry.chemical_elementPHYTOCHELATIN SYNTHASEFungusStressAM fungi; arsenic; cadmium; cigarettes; glutathione; mycorrhiza; smoking; tobaccoArsenicSymbiosisStress PhysiologicalBotanyTobaccomedicineGeneticsTOLERANCEAdaptationGlomeromycotaSymbiosisACCUMULATIONCigarettesfungiL. TYPESGlutathionebiology.organism_classificationPHOSPHATE-UPTAKE SYSTEMTRANSGENIC TOBACCOPlant LeaveschemistryMycorrhiza
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Direct and indirect antioxidant properties of α-lipoic acid and therapeutic potential.

2012

International audience; Diabetes has emerged as a major threat to worldwide health. The exact mechanisms underlying the disease are unknown; however, there is growing evidence that the excess generation of reactive oxygen species (ROS) associated with hyperglycemia, causes oxidative stress in a variety of tissues. In this context, various natural compounds with pleiotropic actions like lipoic acid (LA) are of interest, especially in metabolic diseases such as diabetes. LA, either as a dietary supplement or a therapeutic agent, modulates redox potential because of its ability to match the redox status between different subcellular compartments as well as extracellularly. Both the oxidized (d…

Antioxidantmedicine.medical_treatmentContext (language use)InflammationBiologymedicine.disease_causeAntioxidants03 medical and health scienceschemistry.chemical_compound0302 clinical medicine[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular systemDiabetes mellitusmedicineDiabetes MellitusAnimalsHumans030304 developmental biologyChelating Agentschemistry.chemical_classificationInflammation0303 health sciencesReactive oxygen speciesThioctic AcidEndothelial CellsMetabolismmedicine.disease3. Good health[SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular systemMitochondriaLipoic acidOxidative StresschemistryBiochemistryCardiovascular Diseases030220 oncology & carcinogenesisHyperglycemiaDietary Supplementsmedicine.symptomReactive Oxygen SpeciesOxidation-ReductionOxidative stressFood ScienceBiotechnologyMolecular nutritionfood research
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Controlling protein interactions in blood for effective liver immunosuppressive therapy by silica nanocapsules

2020

Immunosuppression with glucocorticoids is a common treatment for autoimmune liver diseases and after liver transplant, which is however associated with severe side-effects. Targeted delivery of glucocorticoids to inflammatory cells, e.g. liver macrophages and Kupffer cells, is a promising approach for minimizing side effects. Herein, we prepare core–shell silica nanocapsules (SiO2 NCs) via a sol–gel process confined in nanodroplets for targeted delivery of dexamethasone (DXM) for liver immunosuppressive therapy. DXM with concentrations up to 100 mg mL−1 in olive oil are encapsulated while encapsulation efficiency remains over 95% after 15 days. Internalization of NCs by non-parenchymal muri…

Apolipoprotein BCell SurvivalLiver cytologyPharmacologybehavioral disciplines and activitiesDexamethasoneNanocapsulesProinflammatory cytokine//purl.org/becyt/ford/1 [https]MiceDrug Delivery SystemsDrug StabilityNanocapsulesQuímica Coloidalmental disordersBlood plasma//purl.org/becyt/ford/1.4 [https]AnimalsHumansIMMUNOSUPPRESSIVE THERAPYTissue DistributionGeneral Materials ScienceColloidsImmunosuppression TherapybiologyClusterinChemistryCiencias QuímicasSILICA NANOCAPSULESSilicon DioxideBlood proteinsPROTEIN INTERACTIONSDEXAMETHASONELiverbiology.proteinPEGylationCytokinesCIENCIAS NATURALES Y EXACTASImmunosuppressive AgentsHeLa CellsNanoscale
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Apolipoprotein E Regulates Amyloid Formation within Endosomes of Pigment Cells.

2015

International audience; Accumulation of toxic amyloid oligomers is a key feature in the pathogenesis of amyloid-related diseases. Formation of mature amyloid fibrils is one defense mechanism to neutralize toxic prefibrillar oligomers. This mechanism is notably influenced by apolipoprotein E variants. Cells that produce mature amyloid fibrils to serve physiological functions must exploit specific mechanisms to avoid potential accumulation of toxic species. Pigment cells have tuned their endosomes to maximize the formation of functional amyloid from the protein PMEL. Here, we show that ApoE is associated with intraluminal vesicles (ILV) within endosomes and remain associated with ILVs when th…

Apolipoprotein EAmyloidAmyloidEndosome[SDV.BC]Life Sciences [q-bio]/Cellular BiologyEndosomesBiologyExosomesGeneral Biochemistry Genetics and Molecular BiologyMiceApolipoproteins Emental disordersAnimalsHumansamyloid-related diseaseslcsh:QH301-705.5[SDV.BC] Life Sciences [q-bio]/Cellular BiologyMelanosomeMice KnockoutMelanosomesEndosomal Sorting Complexes Required for TransportVesicleMicrovesiclesPMELCell biologyMice Inbred C57BLlcsh:Biology (General)BiochemistryGene Expression RegulationMelanocytesSignal transductionHeLa CellsSignal TransductionCell reports
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