Search results for "ILL"
showing 10 items of 18080 documents
Resistance of a recombinant Escherichia coli to dehydration.
2009
International audience; Dehydration of microorganisms, rendering them anhydrobiotic, is often an efficient method for the short and long term conservation of different strain-producers. However, some biotechnologically important recombinant bacterial strains are extremely sensitive to conventional treatment. We describe appropriate conditions during dehydration of the recombinant Escherichia coli strain HB 101 (GAPDH) that can result dry cells having a 88% viability on rehydration. The methods entails air-drying after addition of 100 mM trehalose to the cultivation medium or distilled water (for short term incubation).
Assessing Methane Emission and Economic Viability of Energy Exploitation in a Typical Sicilian Municipal Solid Waste Landfill
2018
Sanitary landfills for municipal solid waste (MSW) represent one of the major anthropogenic source of GHGs emissions and are directly responsible of the climate changes we are facing nowadays. Indeed, the biodegradable organic matter of MSW undergoes anaerobic digestion producing the landfill gas (LFG), whose main components are CH4 and CO2. Therefore, biomethane energy exploitation in MSW landfills will reduce GHGs emission positively affecting the global warming. The aim of the present study was to assess the methane production in a Sicilian landfill by comparing the results from field measurements of methane emission and the estimates achieved by applying different mathematical models. A…
Antifungal effect of phenolic extract of fermented rice bran with Rhizopus oryzae and its potential use in loaf bread shelf life extension
2018
In this study the antifungal potential of a phenolic extract obtained from rice bran fermented with Rhizopus oryzae CECT 7560 and its application in the elaboration of bread was assessed.; Results: Eighteen compounds with antifungal potential were identified by LC-ESI-qTOF-MS in the extract: organic acids, gallates and gallotannins, flavonoids, ellagic acid and benzophenone derivatives. The extract was active against strains of Fusarium, Aspergillus and Penicillium, with minimum inhibitory concentration ranging from 390 to 3100 µg mL-1 and minimum fungicidal concentration variable from 780 to 6300 µg mL-1 . The strains that were most sensitive to the phenolic extract were F. graminearum, F.…
No apparent effect of invasive alien goldenrod on soil microbial communities or soil fauna feeding activity
2020
Abstract Invasive alien species spreading in abandoned agricultural landscape may negatively affect native organisms, including plants and soil biota. This study assessed the effects of invasion by Solidago sp. (goldenrod) in southern Poland on soil fauna feeding activity and on soil microorganism activity, biomass, and functional and taxonomic diversity, in a comparisons of paired plots: goldenrod-invaded plots and nearby non-invaded plots of semi-natural grassland. Goldenrod-invaded plots had fewer vascular plant species (paired t-test) than non-invaded ones, and showed a distinct plant community composition (ANOSIM). Non-invaded and invaded plots did not differ in a range of measured soi…
Development of a Bioactive Sauce Based on Oriental Mustard Flour with Antifungal Properties for Pita Bread Shelf Life Improvement
2019
Ochratoxin A (OTA) is a mycotoxin produced in the secondary metabolism of fungus belonging to the genus Aspergillus and Penicillium. In this study, the employment of oriental mustard flour (OMF) as an ingredient in a packaged sauce was evaluated for the generation in situ of the antimicrobial compound allyl isothiocyanate (AITC) in order to preserve pita bread contaminated with Penicillium verrucosum VTT D-01847, an OTA producer, in an active packaging system. Four different concentrations (8, 16, 33 and 50 mg/g) were tested. Mycelium formation, mycotoxin production, AITC absorbed by the food matrix, and volatilization kinetics were studied for each concentration. The results obtained were …
Antifungal and antimycotoxigenic activity of allyl isothiocyanate on barley under different storage conditions
2019
Abstract The present study evaluated the efficacy of allyl isothiocyanate (AITC) in avoiding the growth of Penicillium verrucosum and consequent ochratoxin A (OTA) production in barley during storage for 90 d. Environmental humidity was controlled using saturated salt solutions and moisture content (MC) of the grain was analyzed. Moreover, the residual concentration of AITC on stored barley was also examined. Samples with 20.6% of MC presented the highest absorption of AITC with levels ranging from 75 to 4 mg/kg at day 1 and 90, respectively. The population of P. verrucosum was significantly reduced after 24 h of AITC exposure. After 90 d, the non-treated control group reached a fungal popu…
Efficiency of Ohmic assisted hydrodistillation for the extraction of essential oil from oregano (Origanum vulgare subsp. viride) spices
2017
Abstract The aim of this work was to investigate the effects of Ohmic heating-assisted hydrodistillation (OAHD), applied at different voltages (100, 150, and 200) V, on the qualitative and quantitative characteristics of extracted essential oil from oregano herb ( Origanum vulgare subsp. viride ), compared to a conventional hydrodistillation (HD) procedure. The compositions of the extracted essential oils (using OAHD and HD) were assessed using gas chromatography - mass spectrometry (GC–MS). A 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) method was applied in order to evaluate antioxidant activity. The antimicrobial activity as well as the minimum inhibitory concentration (MIC) values …
Poplar Biochar as an Alternative Substrate for Curly Endive Cultivated in a Soilless System
2020
Imminent necessity for eco-friendly and low-cost substitutes to peat is a defiance in the soilless plant cultivation systems. Wood biochar could entirely or partly substitute peat as a plant growing constituent to produce vegetables. Nevertheless, knowledge concerning potential plant performance of leafy green vegetables grown on wood biochar is restricted. The present study assessed the main physicochemical traits of various growing media constituted by decreasing the content of peat and by increasing the percentages of poplar wood biochar. Yield, nutritional and functional properties of curly endive plants cultivated in a protected environment were also tested. Biochar was pyrolyzed from …
Chlamyphilone, a Novel Pochonia chlamydosporia Metabolite with Insecticidal Activity
2019
Metabolites from a collection of selected fungal isolates have been screened for insecticidal activity against the aphid Acyrthosiphon pisum. Crude organic extracts of culture filtrates from six fungal isolates (Paecilomyces lilacinus, Pochonia chlamydosporia, Penicillium griseofulvum, Beauveria bassiana, Metarhizium anisopliae and Talaromyces pinophilus) caused mortality of aphids within 72 h after treatment. In this work, bioassay-guided fractionation has been used to characterize the main bioactive metabolites accumulated in fungal extracts. Leucinostatins A, B and D represent the bioactive compounds produced by P. lilacinus. From P. griseofulvum and B. bassiana extracts, griseofulvin an…
RNA interference in Lepidoptera: an overview of successful and unsuccessful studies and implications for experimental design.
2011
International audience; Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involv…