Search results for "Invasive"

showing 10 items of 1141 documents

Downregulation of intratumoral expression of miR-205, miR-200c and miR-125b in primary human cutaneous melanomas predicts shorter survival

2018

AbstractWhile only 15–25 percent of melanoma patients develop distant metastasis and die, this disease is still responsible for the majority of skin cancer-related deaths. The availability of adjuvant therapies makes the selection of high-risk patients essential. We evaluated the intratumoral expression of ten miRNAs in primary melanomas in relation to its ability to predict melanoma survival. To this end, we correlated miRNA expression in 132 cryopreserved primary and metastatic tumors with clinicopathological factors and clinical outcome. We found sequential downregulation of intratumoral expression of miR-125b, miR-182, miR-200c and miR-205 over the full spectrum of melanoma progression.…

Male0301 basic medicineSkin Neoplasmsmedicine.medical_treatmentlcsh:MedicineDown-RegulationDiseaseArticleMetastasisExtracellular matrix03 medical and health sciences0302 clinical medicineDownregulation and upregulationCell MovementmicroRNABiomarkers TumorTumor Cells CulturedmedicineHumansNeoplasm Invasivenesslcsh:ScienceMelanomaAgedCell ProliferationMultidisciplinarybusiness.industryMelanomalcsh:RPrognosismedicine.diseaseIn vitroGene Expression Regulation NeoplasticSurvival RateMicroRNAs030104 developmental biology030220 oncology & carcinogenesisDisease ProgressionCancer researchlcsh:QFemalebusinessAdjuvantFollow-Up StudiesScientific Reports
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Studying Closed Hydrodynamic Models of "In Vivo" DNA Perfusion in Pig Liver for Gene Therapy Translation to Humans.

2016

17 páginas, 6 figuras. En la versión online contiene 3 figuras y 1 tabla en información suplemetaria

Male0301 basic medicineSwineCardiovascular ProceduresGenetic enhancementProtein ExpressionCellGene ExpressionMetal Nanoparticleslcsh:MedicineVascular SurgeryBiochemistryTranslational Research BiomedicalMice0302 clinical medicinePig ModelsGene expressionMedicine and Health SciencesTransgeneslcsh:ScienceMammalsMultidisciplinaryPhysicsGene Transfer TechniquesClassical MechanicsAgricultureAnimal ModelsPerfusionmedicine.anatomical_structureLivermedicine.veinOrgan SpecificityNaked DNA030220 oncology & carcinogenesisVertebratesPhysical SciencesFemalePerfusionPlasmidsResearch ArticleLivestockSurgical and Invasive Medical ProceduresFluid MechanicsBiologyGene deliveryResearch and Analysis MethodsContinuum MechanicsInferior vena cavaCatheterizationGene Delivery03 medical and health sciencesModel OrganismsIn vivoGene Expression and Vector TechniquesmedicineAnimalsHumansMolecular Biology TechniquesMolecular BiologyMolecular Biology Assays and Analysis TechniquesPlasma Proteinslcsh:ROrganismsBiology and Life SciencesProteinsFluid DynamicsDNAGenetic TherapyMolecular biology030104 developmental biologyalpha 1-AntitrypsinAmniotesHydrodynamicslcsh:QGoldPLoS ONE
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Clinical application of embryo aneuploidy testing by next-generation sequencing

2019

Abstract We review here the evolution in the field of embryo aneuploidy testing over the last 20 years, from the analysis of a subset of chromosomes by fluorescence in situ hybridisation to the transition toward a more comprehensive analysis of all 24 chromosomes. This current comprehensive aneuploidy testing most commonly employs next-generation sequencing (NGS). We present our experience in over 130 000 embryo biopsies using this technology. The incidence of aneuploidy was lower in trophectoderm biopsies compared to cleavage-stage biopsies. We also confirmed by NGS that embryo aneuploidy rates increased with increasing maternal age, mostly attributable to an increase in complex aneuploid …

Male0301 basic medicineTime FactorsNoninvasive Prenatal TestingAneuploidySingle Embryo TransferBiologyMiscarriageAndrology03 medical and health sciences0302 clinical medicinePregnancyRisk FactorsRecurrent miscarriagemedicineHumansGenetic TestingBlastocystPrecision MedicinePreimplantation DiagnosisGenetic testingPregnancy030219 obstetrics & reproductive medicinemedicine.diagnostic_testMosaicismHigh-Throughput Nucleotide SequencingCell BiologyGeneral MedicineAneuploidyEmbryo Transfermedicine.diseaseEmbryo transferBlastocyst030104 developmental biologymedicine.anatomical_structureReproductive MedicineCytogenetic AnalysisFemaleCell-Free Nucleic AcidsBiology of Reproduction
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Microcirculatory Assessment of Arterial Below-Knee Stumps: Near-Infrared Spectroscopy Versus Transcutaneous Oxygen Tension—A Preliminary Study in Pro…

2017

International audience; Objective: To examine metrologic properties of near-infrared spectroscopy (NIRS) versus transcutaneous oxygen tension (TcPO2) for micro-circulatory assessment of vascular transtibial stumps at the stabilized period of prosthesis fitting, as a preliminary step before exploring its ability to predict stump healing, considering the previously identified limits of TcPO2 (borderline area between 15 and 35mmHg).Design: Prospective single-center observational study.Setting: University-based rehabilitation center.Participants: Individuals with unilateral transtibial amputation for peripheral artery disease, at the definitive stage of prosthesis fitting, able to perform a 2-m…

Male030506 rehabilitationFuture studiesSupine positionArterial diseaseIntraclass correlationmedicine.medical_treatmentamputees030204 cardiovascular system & hematologynear-infraredProsthesischemistry.chemical_compound0302 clinical medicinenoninvasive methodsMedicineProspective StudiesSpectroscopyAged 80 and overSpectroscopy Near-InfraredAmputation stumpsexerciseRehabilitationMiddle Aged[ SDV.MHEP.CSC ] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system3. Good healthsurgical procedures operativeAnesthesiaFemale0305 other medical scienceAdultcritical limb ischemiamedicine.medical_specialtyVisual analogue scaleArtificial LimbsPhysical Therapy Sports Therapy and RehabilitationAmputation SurgicalBlood gas monitoringPeripheral Arterial Disease03 medical and health sciences[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular systemlevelpressure measurements[ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathologyHumansTCPOPhysical Therapy ModalitiesAgedbusiness.industryMicrocirculationtest-retest reliabilityReproducibility of ResultsbalancetranscutaneousSurgeryOxygenperipheral vascular-diseasechemistrylower-extremity amputationTranscutaneous oxygenbusiness[SDV.MHEP]Life Sciences [q-bio]/Human health and pathologyArchives of Physical Medicine and Rehabilitation
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Predictors of non-invasive ventilation tolerance in amyotrophic lateral sclerosis

2011

Abstract Background The most frequent cause of death in patients with Amyotrophic Lateral Sclerosis (ALS) is respiratory failure. Recently, it has been shown that non-invasive ventilation improves survival and quality of life in ALS patients with respiratory failure, but little is known about predictors of non-invasive ventilation adaptation and tolerance. In this study we evaluated the effect of a comprehensive information about non-invasive ventilation use and a prolonged and intensive monitoring on tolerance to this palliative care. Methods We prospectively monitored all consecutive ALS patients with chronic respiratory failure and indication to non-invasive ventilation between January 2…

MaleALS Chronic respiratory failure Tolerance to non-invasive ventilation Bulbar impairment POSITIVE-PRESSURE VENTILATION QUALITY-OF-LIFE PRACTICE PARAMETER CARE MANAGEMENT STANDARDS BIPAPmedicine.medical_specialtyPalliative carePatientsTolerance to non-invasive ventilationBody Mass IndexQuality of lifeQUALITY-OF-LIFEPRACTICE PARAMETERMANAGEMENTHumansMedicineDementiaChronic respiratory failureOximetryProspective StudiesAmyotrophic lateral sclerosisIntensive care medicineBIPAPAgedCause of deathVentilators Mechanicaltolerancebusiness.industryAmyotrophic Lateral SclerosisRespiratory diseaseSialorrheaCAREMiddle Agedmedicine.diseaseRespiration ArtificialBulbar impairment POSITIVE-PRESSURE VENTILATIONCaregiversNeurologyRespiratory failureSpirometryChronic DiseaseNIVEmergency medicineBreathingFemaleSettore MED/26 - NeurologiaNeurology (clinical)Blood Gas AnalysisALSRespiratory InsufficiencybusinessSTANDARDSJournal of the Neurological Sciences
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Geo-economic variations in epidemiology, patterns of care, and outcomes in patients with acute respiratory distress syndrome: insights from the LUNG …

2017

Background Little information is available about the geo-economic variations in demographics, management, and outcomes of patients with acute respiratory distress syndrome (ARDS). We aimed to characterise the effect of these geo-economic variations in patients enrolled in the Large Observational Study to Understand the Global Impact of Severe Acute Respiratory Failure (LUNG SAFE). Methods LUNG SAFE was done during 4 consecutive weeks in winter, 2014, in a convenience sample of 459 intensive-care units in 50 countries across six continents. Inclusion criteria were admission to a participating intensive-care unit (including transfers) within the enrolment window and receipt of invasive or non…

MaleARDSdemographyeconomicmedicine.medical_treatmentTerapéuticaair conditioningComorbidityintensive care unitdeveloped country0302 clinical medicineneuromuscular blockingmiddle agedacute myocardial-infarctionmiddle income countryProspective StudiesGeography Medicalcritically-ill patientsadultagedpriority journalrisk factorIncomegeographic-variationDeveloped countryhospitalizationprospective studyHumanPulmonary and Respiratory MedicineDeveloped Countriemedicine.medical_specialtyDeveloping countryArticle/dk/atira/pure/subjectarea/asjc/2700/274003 medical and health sciencesSíndrome respiratorio agudo graveunitsMedicalHumansIntensive care medicineDeveloping CountriesAgedhigh income countryRespiratory Distress Syndrome Adultnoninvasive ventilationAparato respiratoriomedicine.diseasemortalitymajor clinical studyProspective Studiearterial oxygen tension030228 respiratory systemARDSObservational studySociologíahealth care deliverygeographyintensive-careRisk FactorsEpidemiologyProspective cohort studyRespiratory Distress Syndromepartial pressureartificial ventilationSociología médicaMiddle Agedadult respiratory distress syndromeAged; Comorbidity; Delivery of Health Care; Developed Countries; Developing Countries; Europe; Female; Geography Medical; Humans; Income; Intensive Care Units; Male; Middle Aged; Patient Outcome Assessment; Prospective Studies; Respiratory Distress Syndrome Adult; Risk Factors; Pulmonary and Respiratory MedicineEuropeIntensive Care UnitsfemaleincomeFemaleEnfermedadinjurycohort analysigross national incomesurvivalNOmedical geographyDeveloping Countrielength of staymedicinecontrolled studyoutcome assessmentbreast-cancerMechanical ventilationdiseasebusiness.industryDeveloped Countriespatient caredeveloping country030208 emergency & critical care medicinestatistics and numerical data AgedComorbiditywinterACUTE MYOCARDIAL-INFARCTION; CRITICALLY-ILL PATIENTS; GEOGRAPHIC-VARIATION; INTENSIVE-CARE; BREAST-CANCER; MORTALITY; DISEASE; INJURY; UNITS; HOSPITALIZATIONPatient Outcome AssessmentEmergency medicineprone positiontreatment outcomebusinessDelivery of Health Care
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Impact of Early Acute Kidney Injury on Management and Outcome in Patients With Acute Respiratory Distress Syndrome: A Secondary Analysis of a Multice…

2019

Contains fulltext : 208648.pdf (Publisher’s version ) (Closed access) OBJECTIVES: To understand the impact of mild-moderate and severe acute kidney injury in patients with acute respiratory distress syndrome. DESIGN: Secondary analysis of the "Large Observational Study to Understand the Global Impact of Severe Acute Respiratory Failure", an international prospective cohort study of patients with severe respiratory failure. SETTING: Four-hundred fifty-nine ICUs from 50 countries across five continents. SUBJECTS: Patients with a glomerular filtration rate greater than 60 mL/min/1.73 m prior to admission who fulfilled criteria of acute respiratory distress syndrome on day 1 and day 2 of acute …

MaleARDSmedicine.medical_treatmentComorbidityCritical Care and Intensive Care MedicineSeverity of Illness Indexchemistry.chemical_compound0302 clinical medicineacute kindey faliureRisk FactorsOdds Ratio80 and overHospital MortalityProspective StudiesAged 80 and overRespiratory Distress SyndromeRespirationAcute kidney injuryMiddle Agedacute kidney injury acute respiratory distress syndrome invasive mechanical ventilation mortality renal replacement therapyIntensive Care Unitsacute kidney injuryCreatinineArtificialFemaleGlomerular Filtration RateAdultinvasive mechanical ventilationrenal replacement therapy.medicine.medical_specialtySocio-culturaleRenal functionard03 medical and health sciencesInternal medicineSettore MED/41 - ANESTESIOLOGIASeverity of illnessmedicineHumansacute kidney injury acute respiratory distress syndromeRenal replacement therapyAgedCreatinineRenal replacement therapybusiness.industry030208 emergency & critical care medicineCarbon Dioxideacute respiratory distress syndromemedicine.diseaseRespiration Artificialmortalitylnfectious Diseases and Global Health Radboud Institute for Health Sciences [Radboudumc 4]030228 respiratory systemchemistryRespiratory failurebusinessRespiratory minute volume
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Immunocompromised patients with acute respiratory distress syndrome: Secondary analysis of the LUNG SAFE database

2018

Background: The aim of this study was to describe data on epidemiology, ventilatory management, and outcome of acute respiratory distress syndrome (ARDS) in immunocompromised patients. Methods: We performed a post hoc analysis on the cohort of immunocompromised patients enrolled in the Large Observational Study to Understand the Global Impact of Severe Acute Respiratory Failure (LUNG SAFE) study. The LUNG SAFE study was an international, prospective study including hypoxemic patients in 459 ICUs from 50 countries across 5 continents. Results: Of 2813 patients with ARDS, 584 (20.8%) were immunocompromised, 38.9% of whom had an unspecified cause. Pneumonia, nonpulmonary sepsis, and noncardiog…

MaleARDSmodelos logísticosDatabases Factualmedicine.medical_treatment[SDV]Life Sciences [q-bio]humanoslnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4]Kaplan-Meier EstimateCritical Care and Intensive Care MedicineAcute respiratory failureSeverity of Illness IndexCohort Studiesrandomized-trial0302 clinical medicineMechanical ventilationRisk Factorsestudios prospectivosEpidemiology80 and overicuMedicineProspective StudiesProspective cohort studyestudios de cohortesImmunodeficiencymediana edadestadísticasAged 80 and overRespiratory Distress Syndromeancianocritically-ill patientsRespirationresultado del tratamientorespiraciónStatisticslcsh:Medical emergencies. Critical care. Intensive care. First aidadultoMiddle Aged3. Good healthfailureIntensive Care UnitsTreatment OutcomeArtificialCohortprospective multicenterImmunocompromised patientsAcute respiratory failure; ARDS; Immunocompromised patients; Mechanical ventilation; Noninvasive ventilation; Critical Care and Intensive Care MedicineFemaleNoninvasive ventilationHumanestimación de Kaplan-MeierAdultmedicine.medical_specialtyLogistic ModelIntensive Care UnitSocio-culturaleunidades de cuidados intensivossurvivalStatistics NonparametricSepsisDatabases03 medical and health sciencesImmunocompromised HostInternal medicineImmunocompromised patientcancerfactores de riesgoHumansNonparametricíndice de gravedad de la enfermedadintensive-care-unitFactualAgedMechanical ventilationbusiness.industryResearchRisk FactorRespiratory Distress Syndrome Adult030208 emergency & critical care medicinelcsh:RC86-88.9medicine.diseaseRespiration ArtificialPneumoniaProspective StudieLogistic Models030228 respiratory systemmalignanciesARDShuésped inmunodeprimidoCohort StudiebusinessAcute respiratory failure; ARDS; Immunocompromised patients; Mechanical ventilation; Noninvasive ventilation; Adult; Aged; Aged 80 and over; Cohort Studies; Databases Factual; Female; Humans; Intensive Care Units; Kaplan-Meier Estimate; Logistic Models; Male; Middle Aged; Prospective Studies; Respiration Artificial; Respiratory Distress Syndrome Adult; Risk Factors; Severity of Illness Index; Statistics Nonparametric; Treatment Outcome; Immunocompromised Host
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Noninvasive Ventilation of Patients with Acute Respiratory Distress Syndrome: Insights from the LUNG SAFE Study

2016

Rationale: Noninvasive ventilation (NIV) is increasingly used in patients with acute respiratory distress syndrome (ARDS). The evidence supporting NIV use in patients with ARDS remains relatively sparse.Objectives: To determine whether, during NIV, the categorization of ARDS severity based on the PaO2/FiO2 Berlin criteria is useful.Methods: The LUNG SAFE (Large Observational Study to Understand the Global Impact of Severe Acute Respiratory Failure) study described the management of patients with ARDS. This substudy examines the current practice of NIV use in ARDS, the utility of the PaO2/FiO2 ratio in classifying patients receiving NIV, and the impact of NIV on outcome.Measurements and Main…

MaleARDSprocedureblood oxygen tensionCritical Care and Intensive Care MedicineSeverity of Illness Indexlaw.invention0302 clinical medicinelawHospital MortalityRespiratory Distress SyndromeAcute respiratory distress syndromeadult respiratory distress syndromeMiddle AgedIntensive care unitIntensive Care Unitsmedicine.anatomical_structureTreatment Outcomepriority journalpositive end expiratory pressureNoninvasive ventilationdisease severityFemaletreatment outcome AgedNoninvasive ventilationprospective studyHumanAdultPulmonary and Respiratory Medicinemedicine.medical_specialtycohort analysiIntensive Care Unitdisease classificationAcute respiratory distressArticleNO03 medical and health sciencesacute respiratory distress syndrome; noninvasive ventilationlength of staySeverity of illnessSettore MED/41 - ANESTESIOLOGIAmedicineSequential Organ Failure Assessment ScoreHumansIn patientAcute respiratory distress syndrome; Noninvasive ventilation; Aged; Female; Hospital Mortality; Humans; Intensive Care Units; Male; Middle Aged; Respiratory Distress Syndrome Adult; Severity of Illness Index; Treatment Outcome; Noninvasive Ventilation; Pulmonary and Respiratory Medicine; Critical Care and Intensive Care MedicineIntensive care medicineoutcome assessmentAgedLungbusiness.industryRespiratory Distress Syndrome Adult030208 emergency & critical care medicinemedicine.diseasemajor clinical studymortalityrespiratory tract diseasesbreathing ratemulticenter study030228 respiratory systemincidenceObservational studyobservational studybusinessAcute respiratory distress syndrome; Noninvasive ventilation;
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p38 MAPK Controls Prothrombin Expression by Regulated RNA 3′ End Processing

2011

Thrombin is a key protease involved in blood coagulation, complement activation, inflammation, angiogenesis, and tumor invasion. Although induced in many (patho-)physiological conditions, the underlying mechanisms controlling prothrombin expression remained enigmatic. We have now discovered that prothrombin expression is regulated by a posttranscriptional regulatory mechanism responding to stress and inflammation. This mechanism is triggered by external stimuli that activate p38 MAPK. In turn, p38 MAPK upmodulates canonical 3' end processing components and phosphorylates the RNA-binding proteins FBP2 and FBP3, which inhibit 3' end processing of mRNAs, such as prothrombin mRNA, that bear a d…

MaleAdenosinePolymersp38 mitogen-activated protein kinasesInflammationPlasma protein bindingBiologyp38 Mitogen-Activated Protein KinasesGene Expression Regulation EnzymologicMiceThrombinCell Line TumormedicineAnimalsHumansNeoplasm InvasivenessRNA MessengerMolecular BiologyRegulation of gene expressionMessenger RNARNACell BiologyXenograft Model Antitumor AssaysCell biologyRibonucleoproteinsImmunologyPhosphorylationRNAProthrombinmedicine.symptomRNA 3' End Processingmedicine.drugProtein BindingMolecular Cell
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