Search results for "LAB"
showing 10 items of 7932 documents
Intragenic G-quadruplex structure formed in the human CD133 and its biological and translational relevance.
2016
Cancer stem cells (CSCs) have been identified in several solid malignancies and are now emerging as a plausible target for drug discovery. Beside the questionable existence of CSCs specific markers, the expression of CD133 was reported to be responsible for conferring CSC aggressiveness. Here, we identified two G-rich sequences localized within the introns 3 and 7 of the CD133 gene able to form G-quadruplex (G4) structures, bound and stabilized by small molecules. We further showed that treatment of patient-derived colon CSCs with G4-interacting agents triggers alternative splicing that dramatically impairs the expression of CD133. Interestingly, this is strongly associated with a loss of C…
Metabolic Escape Routes of Cancer Stem Cells and Therapeutic Opportunities
2020
Although improvement in early diagnosis and treatment ameliorated life expectancy of cancer patients, metastatic disease still lacks effective therapeutic approaches. Resistance to anticancer therapies stems from the refractoriness of a subpopulation of cancer cells—termed cancer stem cells (CSCs)—which is endowed with tumor initiation and metastasis formation potential. CSCs are heterogeneous and diverge by phenotypic, functional and metabolic perspectives. Intrinsic as well as extrinsic stimuli dictated by the tumor microenvironment (TME)have critical roles in determining cell metabolic reprogramming from glycolytic toward an oxidative phenotype and vice versa, allowing cancer cells to th…
Dual Inhibition of Myc Transcription and PI3K Activity Effectively Targets Colorectal Cancer Stem Cells
2022
Despite advances in the curative approach, the survival rate of advanced colorectal cancer (CRC) patients is still poor, which is likely due to the emergence of cancer cell clones resistant to the available therapeutic options. We have already shown that CD44v6-positive CRC stem cells (CR-CSCs) are refractory toward standard anti-tumor therapeutic agents due to the activation of the PI3K pathway together with high HER2 expression levels. Tumor microenvironmental cytokines confer resistance to CR-CSCs against HER2/PI3K targeting by enhancing activation of the MAPK pathway. Here, we show that the CSC compartment, spared by BRAF inhibitor-based targeted therapy, is associated with increased ex…
Dissecting the Mechanism of Action of Spiperone—A Candidate for Drug Repurposing for Colorectal Cancer
2022
Approximately 50% of colorectal cancer (CRC) patients still die from recurrence and metastatic disease, highlighting the need for novel therapeutic strategies. Drug repurposing is attracting increasing attention because, compared to traditional de novo drug discovery processes, it may reduce drug development periods and costs. Epidemiological and preclinical evidence support the antitumor activity of antipsychotic drugs. Herein, we dissect the mechanism of action of the typical antipsychotic spiperone in CRC. Spiperone can reduce the clonogenic potential of stem-like CRC cells (CRC-SCs) and induce cell cycle arrest and apoptosis, in both differentiated and CRC-SCs, at clinically relevant co…
Targeting of the Peritumoral Adipose Tissue Microenvironment as an Innovative Antitumor Therapeutic Strategy
2022
The tumor microenvironment (TME) plays a key role in promoting and sustaining cancer growth. Adipose tissue (AT), due to its anatomical distribution, is a prevalent component of TME, and contributes to cancer development and progression. Cancer-associated adipocytes (CAAs), reprogrammed by cancer stem cells (CSCs), drive cancer progression by releasing metabolites and inflammatory adipokines. In this review, we highlight the mechanisms underlying the bidirectional crosstalk among CAAs, CSCs, and stromal cells. Moreover, we focus on the recent advances in the therapeutic targeting of adipocyte-released factors as an innovative strategy to counteract cancer progression.
L'iconografia del candelabro per il cero pasquale della Cappella Palatina, con alcune considerazioni sul committente o donatore
2019
The candelabrum for the Easter candle in the Palatine Chapel in Palermo is in many ways one of the emblematic examples of Norman sculpture in Sicily, to be placed in the context of a broader Mediterranean current, classicistic and highly expressive, which includes contemporary productions of Tuscany, Emilia, Provence, Ile-de-France, Roussillon and Languedoc, Catalonia, up to the Holy Land of the Crusaders. This essay examines its complex iconography and proposes new hypotheses about its probable client or donor, the Archbishop of Salerno and chronicler Romualdo Guarna..
Elementi di pericolosità geologica dell’offshore del margine calabro-ionico
2008
Capillaroscopic and histologic examination of the human labial mucosa: A new non invasive methodology compared with the histological exam
2005
The aim of our study is to compare the capillaroscopic examination in vivo with the histological examination in vitro of labial microcirculation in healthy subjects. Methods. Twenty-nine subjects (19 females, 10 males; mean±SD age 55.61±2.55; range 45-65 years) were examined in our laboratory. The characteristics of labial microcirculation were examined using computerised videomicroscopic techniques and histological exam. Results. The results obtained with both methods showed some analogies: in fact, the differences in capillary diameter were in the range of ±0.3 µ; regarding capillary density as well, a variation of ±2 capillaries per mm2 was found. Comparing the results obtained with both…
Development of a microfluidic design for an automatic lab-on-chip operation
2016
Simple and easy to use are the keys for developing lab-on-chip technology. Here, a new microfluidic circuit has been designed for an automatic lab-on-chip operation (ALOCO) device. This chip used capillary forces for controlled and precise manipulation of liquids, which were loaded in sequence from different flowing directions towards the analysis area. Using the ALOCO design, a non-expert user is able to operate the chip by pipetting liquids into suitable inlet reservoirs. To test this design, microfluidic devices were fabricated using the programmable proximity aperture lithography technique. The operation of the ALOCO chip was characterized from the flow of red-, blue- and un-dyed deioni…
El capital psicológico del líder y su efecto en la capacidad de aprendizaje organizativo, la flexibilidad y el rendimiento laboral del empleado
2022
Colombia tiene como meta convertirse en el país con el mejor sistema educativo de América Latina para el año 2025. Para lograr dicho objetivo, el Ministerio de Educación de Colombia ha desarrollado diferentes estrategias para optimizar el capital humano. Las condiciones psicológicas de sus líderes deben facilitar la generación y capitalización del conocimiento en contextos como el educativo y mejorar la flexibilidad y el rendimiento laboral del empleado para intervenir y modificar estructuras administrativas que cada vez sean más horizontales. En el nivel organizacional, es importante evaluar el papel del capital psicológico del líder y su influencia en la capacidad de aprendizaje organizat…