Search results for "Length"
showing 10 items of 2188 documents
Identification of Colletotrichum species responsible for anthracnose of strawberry based on the internal transcribed spacers of the ribosomal region.
2000
In recent years, different molecular techniques have led to an important progress in the characterisation of Colletotrichum species, but there are no available methods which permit the easy identification of Colletotrichum strains and their assignation to classical species. In the present work, the restriction patterns generated from the region spanning the internal transcribed spacers (ITS1 and ITS2) and the 5.8S rRNA gene, were used to identify a total of 80 strains of Colletotrichum, the majority of them isolated from strawberry. One of the most interesting results derived from this study was the easy and reliable distinction, using the endonuclease MvnI, between Colletotrichum fragariae…
PCR ITS-RFLP: A useful method for identifying filamentous fungi isolates on grapes.
2010
Restriction digestion analysis of the ITS products was tested as an easy method to identify isolates of filamentous fungi on grapes. Endonucleases SduI, HinfI, MseI, HaeIII were used. Endonucleases BfmI, Cfr9I, Hpy188I, MaeII or PspGI were used as necessary to complete discrimination. The 43 species studied generated 42 different composite profiles. Only the species P. thomii and P. glabrum gave the same composite profile. 96.3% strains tested could be differentiated to the species level with only four enzymes. Hundred ninety nine strains of filamentous fungi were isolated from various vineyards in Burgundy and identified by this method. Penicillium (58.5%) was the genus the most frequently…
Post-larval development of the microcotylid monogenean Sparicotyle chrysophrii (Van Beneden and Hesse, 1863): comparison with species of Microcotylid…
2011
Abstract The chronology of post-larval development in S. chrysophrii, a polyopisthocotylean monogenean parasite of the gilthead seabream (Sparus aurata L.), was experimentally studied. It is compared with other species within the Microcotylidae and the Heteraxinidae, including an analysis of the changes in attachment and the growth rate. Gilthead seabreams infected by larvae of S. chrysophrii were killed periodically in order to collect the different developmental stages. Parasite total body length, haptor length, largest clamp width, and total number of clamps were recorded. Specimens of S. chrysophrii in culture conditions at 20 °C became gravid after 26–30 days, with 37 pairs of clamps. …
Second harmonic generation in selenium-metal structures
2009
The article examines the processes of second harmonic generation (SHG) when selenium-metal (Cu) film structures are illuminated by femtosecond radiation (180 fs, 80 MHz) at wavelength 800 – 1000 nm. Selenium-copper structures were obtained by successive thermal evaporation of selenium and copper onto the glass substrate in vacuum. Microanalysis of the film composition was performed to determine amount of copper in thin films. The as-evaporated selenium-copper structures were crystallised by annealing in inert atmosphere at temperature 85°C. Just evaporated as well as annealed thin films were explored. The experiment was performed by confocal microscope [1] where the femtosecond radiation fr…
A Setup for Microscopic Studies of Ultrasounds Effects on Microliters Scale Samples: Analytical, Numerical and Experimental Characterization
2021
International audience; Sonoporation is the process of cell membrane permeabilization, due to exposure to ultrasounds. There is a lack of consensus concerning the mechanisms of sonoporation: Understanding the mechanisms of sonoporation refines the choice of the ultrasonic parameters to be applied on the cells. Cells’ classical exposure systems to ultrasounds have several drawbacks, like the immersion of the cells in large volumes of liquid, the nonhomogeneous acoustic pressure in the large sample, and thus, the necessity for magnetic stirring to somehow homogenize the exposure of the cells. This article reports the development and characterization of a novel system allowing the exposure to …
One-dimensional wavelength multiplexed microscope without objective lens
2009
A new approach aimed to achieve microscopic imaging without objective lenses and based on wavelength multiplexing of the spatial object information is presented. The proposed method is used to develop, construct and experimentally validate a new type of optical microscope having no objective lens and no numerical reconstruction algorithms to allow imaging process. In order to extract the collected spatial information we use a spectrometer as part of our microscope system. Preliminary results are presented while considering two different types of one-dimensional (1-D) objects.
Superresolution imaging of biological nanostructures by spectral precision distance microscopy
2011
For the improved understanding of biological systems on the nanoscale, it is necessary to enhance the resolution of light microscopy in the visible wavelength range beyond the limits of conventional epifluorescence microscopy (optical resolution of about 200 nm laterally, 600 nm axially). Recently, various far-field methods have been developed allowing a substantial increase of resolution ("superresolution microscopy", or "lightoptical nanoscopy"). This opens an avenue to 'nano-image' intact and even living cells, as well as other biostructures like viruses, down to the molecular detail. Thus, it is possible to combine light optical spatial nanoscale information with ultrastructure analyses…
Suspended core tellurite glass optical fibers for infrared supercontinuum generation
2011
International audience; We report the fabrication and characterization of tellurite TeO(2)-ZnO-Na(2)O (TZN) microstructured suspended core optical fibers (MOFs). These fibers are designed for infrared supercontinuum generation with zero dispersion wavelength (ZDW) at 1.451 mu m. The measured losses at this wavelength are approximately 6 dB/m for a MOF with a 2.2 mu m diameter core. The effective area of a particular fiber is 3.5 mu m(2) and the nonlinear coefficient is calculated to be 437 W(-1)km(-1). By pumping a 20 cm long fiber at 1.56 mu m with a sub-nj femtosecond laser source, we generate a supercontinuum (SC) spanning over 800 nm in the 1-2 mu m wavelength range.
Fluorescence Probes Exhibit Photoinduced Structural Planarization: Sensing <i>in vitro</i> and <i>in vivo</i> Microscopic Dyn…
2019
We demonstrate the construction of wavelength λ-ratiometric images that allow visualizing the distribution of microscopic dynamics within living cells and tissues by using the newly developed principle of fluorescence response. The bent-to-planar motion in the excited state of incorporated fluorescence probes leads to elongation of the π-delocalization, resulting in microviscosity-dependent but polarity-insensitive interplay between well-separated blue and red bands in emission spectra. This allows constructing the exceptionally contrasted images of cellular dynamics. Moreover, the application of probes with increased affinity towards biological membranes allowed detecting the differences i…
The Effects of Acceptance and Commitment Therapy (ACT) Intervention on Inflammation and Stress Biomarkers : a Randomized Controlled Trial
2020
Abstract Background Psychological processes can be manifested in physiological health. We investigated whether acceptance and commitment therapy (ACT), targeted on psychological flexibility (PF), influences inflammation and stress biomarkers among working-age adults with psychological distress and overweight/obesity. Method Participants were randomized into three parallel groups: (1) ACT-based face-to-face (n = 65; six group sessions led by a psychologist), (2) ACT-based mobile (n = 73; one group session and mobile app), and (3) control (n = 66; only the measurements). Systemic inflammation and stress markers were analyzed at baseline, at 10 weeks after the baseline (post-intervention), and…