Search results for "Ligo"
showing 10 items of 1427 documents
Latgales reģionā cirkulējošo Mycobacterium tuberculosis genotipu raksturojums
2019
Tuberkuloze ir pasaulē izplatīta infekcijas slimība, kuras izraisītājas ir M. tuberculosis kompleksā ietilpstošās mikobaktērijas. Darbs tika veikts, lai noteiktu un raksturotu Latgales reģionā cirkulējošo M. tuberculosis genotipus. Darbā ir izmantotas molekulāras genotipēšanas metodes: starpfragmentu oligonukleotīdu genotipēšanas metode, kas ir balstīta uz polimerāzes ķēdes reakciju un tiešā atkārtojuma (DR) reģiona amplificēšanu; restrikcijas fragmentu garuma polimorfisma analīzes metode, kur M. tuberculosis genotips tiek noteikts pēc insercijas sekvenču IS6110 kopiju skaita un izvietojuma atšķirībām hromosomā. Rezultātos tiek atspoguļots, kādas līnijas un apakšlīnijas atrastas Latgales re…
Qualitative mucin disorders in patients with primary Sjögren's syndrome:a literature review
2020
Background It is a common opinion that Primary Sjögren Syndrome (pSS) damages the exocrine glands and determines the reduction of secreted saliva, some studies show that there are qualitative anomalies of the mucins produced in saliva, including MUC7, MUC5B, MUC1. The purpose of this study is to trace all the information useful to establish whether there is a qualitative or quantitative defect of the mucins in the pSS. Material and Methods We reviewed the literature by looking for publications relevant to the topic in electronic databases. Sixteen articles met the search criteria. The studies were divided into two categories, those that studied the rheological characteristics of the saliva …
Agenesias dentarias: en busca de las alteraciones genéticas responsables de la falta de desarrollo
2004
En conjunto, las agenesias dentarias son la malformación cráneofacial más frecuente. Su prevalencia alcanza el 20% en la dentición permanente, y su expresión puede variar desde la ausencia de una sola pieza, generalmente un tercer molar, hasta la de toda la dentición. En la pasada década, los estudios de ligamiento genético y biología molecular han permitido identificar algunas mutaciones responsables de distintos patrones de agenesias dentarias sindrómicas y no sindrómicas. Dichas mutaciones se encuentran en genes clave para el desarrollo de la dentición, como los que codifican a los factores de transcripción MSX1, PAX9 y PITX2, la proteína de señalización EDA y su receptor EDAR. Los estud…
R&D with spillovers: Monopoly versus noncooperative and cooperative duopoly.
2011
This paper compares industry profit and R&D propensity for a duopoly conducting either noncooperative or cooperative R&D and a monopoly, using two different basic models of strategic R&D. One postulates spillovers in R&D inputs and predicts that equilibrium joint profit and R&D levels are always larger under monopoly. The other postulates spillovers in R&D outputs and sometimes predicts that joint profit and R&D levels are larger under either of the alternative scenarios. In addition, unlike input spillovers, spillovers in R&D outputs sometimes exert a positive effect on both effective and private noncooperative R&D levels.
Water-Soluble Cuprizone Derivative: Synthesis, Characterization, and in Vitro Studies
2019
The cuprizone mouse model is one of the most accepted model systems for the investigation of oligodendrocyte degeneration, a process critically involved in the pathogenesis of diseases such as multiple sclerosis or schizophrenia. In order to substitute the in vivo experiments by in vitro approaches, the amine derivative BiMPi is introduced as a water-soluble alternative to cuprizone. Regarding superoxide dismutase activity, toxicity for oligodendrocytes, and disturbance of mitochondrial membrane potential, BiMPi shows similar in vitro effects as is observed in vivo for cuprizone. peerReviewed
The Role of Small-Angle X-Ray Scattering and Molecular Simulations in 3D Structure Elucidation of a DNA Aptamer Against Lung Cancer
2021
Aptamers are short, single-stranded DNA or RNA oligonucleotide molecules that function as synthetic analogs of antibodies and bind to a target molecule with high specificity. Aptamer affinity entirely depends on its tertiary structure and charge distribution. Therefore, length and structure optimization are essential for increasing aptamer specificity and affinity. Here we present a general optimization procedure for finding most populated atomistic structures of DNA aptamers. Based on the existed aptamer LC-18 for lung adenocarcinoma, a new truncated aptamer LC-18t was developed. A three-dimensional shape of LC-18t was reported based on small-angle X-ray scattering (SAXS) experiments and m…
Terapia Celular en Enfermedades Desmielinizantes
2021
Antecedentes y estado actual del tema: La Esclerosis Múltiple es una enfermedad autoinmune y neurodegenerativa crónica de base inflamatoria que afecta a la sustancia gris y blanca del sistema nervioso central. Se caracteriza por la presencia de lesiones focales desmielinizantes y afectación difusa del tejido nervioso aparentemente normal, siendo la discapacidad consecuencia de la neurodegeneración progresiva, a la que se superpone la discapacidad que pueda aparecer como consecuencia de un brote. Los llamados tratamientos modificadores de la Esclerosis Múltiple limitan los brotes de inflamación que acontecen en la forma remitente-recurrente pero apenas modifican la progresión de la discapaci…
Desarrollo y efecto antitumoral de vacunas génicas celulares y silenciamiento génico
2015
La presente tesis utiliza las vacunas antitumorales basadas en células modificadas genéticamente como principal estrategia para intentar lograr una respuesta inmune antitumoral eficaz. La vacunación preventiva con células tumorales modificadas genéticamente ha logrado muy buenos resultados en ratones consiguiendo la inhibición del crecimiento tumoral y la supervivencia final de los mismos. Sin embargo, en la clínica la disponibilidad de ciertas células tumorales es muy baja y su transfección es poco reproducible. Por ello, en esta tesis se plantea como uno de los objetivos principales desarrollar y evaluar el efecto antitumoral de vacunas basadas en complejos celulares formados por células …
A library of monoalkenylsilsesquioxanes as potential comonomers for synthesis of hybrid materials
2018
In this paper we demonstrate a facile and efficient synthesis of monoalkenylsubstituted POSS compounds, i.e., R7(Si8O12)-alkenyl and R7(Si8O12)(OSiMe2)-alkenyl with a functional alkenyl group of chain length (from vinyl to dec-9-enyl, with or without additional –OSiMe2– spacer between the silsesquioxane core and alkenyl moiety) and with five types of inert groups (Et, iBu, iOc, Cy, Ph) at the POSS core. These compounds constitute a library of monosubstituted POSS systems, including thorough spectroscopic and thermal characteristics. These findings could be especially useful for material chemists applying the compounds as modifiers or comonomers for copolymerization reaction, e.g., with olef…
Evolutionary advantage conferred by an eukaryote-to-eukaryote gene transfer event in wine yeasts
2015
Although an increasing number of horizontal gene transfers have been reported in eukaryotes, experimental evidence for their adaptive value is lacking. Here, we report the recent transfer of a 158-kb genomic region between Torulaspora microellipsoides and Saccharomyces cerevisiae wine yeasts or closely related strains. This genomic region has undergone several rearrangements in S. cerevisiae strains, including gene loss and gene conversion between two tandemly duplicated FOT genes encoding oligopeptide transporters. We show that FOT genes confer a strong competitive advantage during grape must fermentation by increasing the number and diversity of oligopeptides that yeast can utilize as a s…