Search results for "Methicillin"

showing 10 items of 60 documents

Dispersal of methicillin resistant Staphylococcus aureus (MRSA) in a burn intensive care unit.

2003

Methicillin resistant Staphylococcus aureus (MRSA) is a pathogen of special concern in intensive care units (ICUs). The burn units are a very susceptible habitat to colonization and infection events by this organism. In this paper isolation of MRSA from a sepsis case and from samples of the care unit air is described, along with simultaneous circulation of two clones of MRSA. Some peculiar epidemiological features of MRSA in burn intensive care wards are confirmed.

Cross InfectionIntensive Care UnitsStaphylococcus aureusBurn UnitsHumansMethicillin Resistancemethicillin resistant staphylococcus aureusStaphylococcal InfectionsSettore MED/42 - Igiene Generale E ApplicataBurnsAnnali di igiene : medicina preventiva e di comunita
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Characterization of a Catalase-Negative Methicillin-Resistant Staphylococcus aureus Strain

2007

ABSTRACT We describe an unusual clinical strain of catalase-negative methicillin-resistant Staphylococcus aureus sensu stricto. Sequence analysis of its catalase gene showed 99.60% identities to the catalase genes of the reference strains. A 5-base deletion, however, led to a shift of the nucleotide reading frame and a loss of the enzymatic activity.

DNA BacterialMaleMicrobiology (medical)Staphylococcus aureusSequence analysisMolecular Sequence DataBiologymedicine.disease_causeStaphylococcal infectionsMicrobiologyparasitic diseasesmedicineHumansGeneAgedchemistry.chemical_classificationStrain (chemistry)BacteriologySequence Analysis DNAStaphylococcal InfectionsCatalasebacterial infections and mycosesmedicine.diseaseMethicillin-resistant Staphylococcus aureusEnzymechemistryStaphylococcus aureusCatalasebiology.proteinMethicillin ResistanceJournal of Clinical Microbiology
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dltA overexpression: A strain-independent keystone of daptomycin resistance in methicillin-resistant Staphylococcus aureus

2013

The mechanisms leading to reduced susceptibility to daptomycin (DAP) are multifactorial and have not been fully elucidated. We analysed, by sequencing and expression studies, the role of the major molecular targets (cell-envelope charge genes, dltA, mprF, cls2; cell-wall turnover and autolysis genes, sceD, atl) involved in the emergence of DAP resistance in three series of isogenic clinical methicillin-resistant Staphylococcus aureus (MRSA) in which DAP resistance emerged after a heterogeneous glycopeptide-intermediate S. aureus (hGISA) step under teicoplanin and DAP therapy. All of the isolates had different genotypes and were delta-haemolysin negative, reflecting a strain proclivity to ac…

DNA BacterialMethicillin-Resistant Staphylococcus aureusMicrobiology (medical)Settore MED/07 - Microbiologia E Microbiologia ClinicaGenotypemedicine.drug_classAntibioticsGene ExpressionBiologyReal-Time Polymerase Chain Reactionmedicine.disease_causeStaphylococcal infectionsMicrobiologyDaptomycinDrug Resistance BacterialmedicineHumansPharmacology (medical)Carbon-Oxygen LigasesGeneTeicoplaninSequence Analysis DNAGeneral MedicineStaphylococcal Infectionsmedicine.diseaseMethicillin-resistant Staphylococcus aureusGlycopeptideMRSA daptomycin resistanceAnti-Bacterial AgentsInfectious DiseasesStaphylococcus aureusMutationDaptomycinmedicine.drug
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Methicillin-resistant Staphylococcus pseudintermedius as causative agent of dairy cow mastitis.

2013

Staphylococcus pseudintermedius is a coagulase-positive specie similar to Staphylococcus intermedius , frequently associated with pyoderma, otitis and urinary tract infections of dogs and cats (van Duijkeren and others 2011). No information about bovine mastitis caused by S pseudintermedius is available in the literature. Antimicrobial resistance among S pseudintermedius strains is increasing: in the past, susceptibility to most antibiotics was common (Bond and Loeffler 2012), but in the last few years methicillin-resistant S pseudintermedius (MRSP) strains have emerged as a significant animal health problem in veterinary medicine (Schwarz and others 2008, van Duijkeren and others 2008, Wee…

DNA BacterialSettore MED/07 - Microbiologia E Microbiologia ClinicaVeterinary medicineStaphylococcus pseudintermediusStaphylococcusPyodermaMicrobial Sensitivity Testsmedicine.disease_causeMicrobiologyMicrobiologyAntibiotic resistancemedicinePrevalenceDairy cattleAnimalsMastitis BovineBacteriological TechniquesMastitiGeneral VeterinarybiologyStaphylococcus intermediusbusiness.industrySCCmecGeneral MedicineStaphylococcal Infectionsbiology.organism_classificationmedicine.diseaseMastitisStaphylococcus aureusHerdCattleFemaleMethicillin ResistancebusinessThe Veterinary record
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Epidemiology of intra-abdominal infection and sepsis in critically ill patients: "AbSeS", a multinational observational cohort study and ESICM Trials…

2019

Pardo-Oviedo, Juan Mauricio/0000-0003-0084-3449; Lopez-Delgado, Juan Carlos/0000-0003-3324-1129; Corradi, Francesco/0000-0002-5588-2608; De Backer, Daniel/0000-0001-9841-5762; POTA, VINCENZO/0000-0001-9999-3388; Tomescu, Dana/0000-0001-9673-5754; Sabetian, Golnar/0000-0001-8764-2150; Girardis, Massimo/0000-0002-2453-0829; Brazzi, Luca/0000-0001-7059-0622; Leone, Marc/0000-0002-3097-758X; Zabolotskikh, Igor Borisovich/0000-0002-3623-2546; De Lange, Dylan/0000-0002-0191-7270; ALMEKHLAFI, GHALEB A./0000-0002-0323-7025; Elke, Gunnar/0000-0002-4948-1605; Grigoras, Ioana/0000-0001-9412-9574; Czuczwar, Miroslaw/0000-0002-9025-6717; Nora, David/0000-0002-1133-7368; Masjedi, Mansoor/0000-0001-6175-9…

Infection riskMaleBIOMEDICINE AND HEALTHCARE. Clinical Medical Sciences.Antibiotic resistanceTracte gastrointestinal - MalaltiesDefinitionsCritical Care and Intensive Care MedicineTHERAPYDEFINITIONS:Infections::Intraabdominal Infections [DISEASES]0302 clinical medicineIntensive care; Intra-abdominal infection; Mortality; Multidrug resistance; Peritonitis; Sepsis;[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseasesObservational studySeptic shockIntensive care; Intra-abdominal infection; Mortality; Multidrug resistance; Peritonitis; SepsisComputingMilieux_MISCELLANEOUSCritical Illness/epidemiology[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseasesBIOMEDICINA I ZDRAVSTVO. Kliničke medicinske znanosti.Intraabdominal Infections/epidemiologyAbdominal infectionMulticenter study3. Good healthManagementClinical trialCohort[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/VirologyCohort analysisCommunity acquired infectionCohort studyHumanmedicine.medical_specialtyCarbapenem resistanceCritical IllnessPeritoneal dialysisPeritonitisVancomycin resistant enterococcusMajor clinical studyPeritonitisArticle03 medical and health sciencesAntibiotic resistance[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular systemIntensive careSepsisSettore MED/41 - ANESTESIOLOGIAHumansCritical care medicineHospital infectionAgedScience & TechnologyLiver failureAntibiotic therapymedicine.disease[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/BacteriologyEpidemiologic Studies030228 respiratory systemIntensive Care Unit; Sepsis (Diptera); Septic ShockRisk factorHuman medicineGeneral & internal medicineCongestive heart failureOriginalMultidrug resistanceCohort StudiesRisk FactorsCause of DeathEpidemiologyPrevalenceMedicine and Health SciencesAbdominal abscessSepsis/epidemiologyMiddle agedAntifungal therapy2. Zero hungerPeritonitiAntibiotic agentBiliary tract infectionIntensive care ; Intra-abdominal infection ; Mortality ; Multidrug resistance ; Peritonitis ; SepsisMiddle Aged:infecciones bacterianas y micosis::infección::infecciones intraabdominales [ENFERMEDADES]PREVALENCE:Infections::Sepsis [DISEASES]:técnicas de investigación::métodos epidemiológicos::características de los estudios epidemiológicos::estudios epidemiológicos::estudios de cohortes [TÉCNICAS Y EQUIPOS ANALÍTICOS DIAGNÓSTICOS Y TERAPÉUTICOS]Methicillin resistant staphylococcus aureusRaonament basat en casosFemaleCritically ill patientLife Sciences & BiomedicineAntifungal agentAdult:Investigative Techniques::Epidemiologic Methods::Epidemiologic Study Characteristics::Epidemiologic Studies::Cohort Studies [ANALYTICAL DIAGNOSTIC AND THERAPEUTIC TECHNIQUES AND EQUIPMENT]Predictive value:infecciones bacterianas y micosis::infección::sepsis [ENFERMEDADES]NOSepsisIntra-abdominal infectionCritical Care MedicineInternal medicineGeneral & Internal MedicinemedicineMANAGEMENTJournal Article[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/ParasitologySepticèmiaMortalitybusiness.industrySeptic shockPancreas diseaseMalnutrition030208 emergency & critical care medicineTyphlitisToxic megacolonIntensive careIntraabdominal InfectionsTherapyLate onset disorderbusiness
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Etestw versus broth microdilution for ceftaroline MIC determination with Staphylococcus aureus: Results from PREMIUM, a European multicentre study

2017

Objectives: To compare the concordance of ceftaroline MIC values by reference broth microdilution (BMD) and Etest (bioMérieux, France) for MSSA and MRSA isolates obtained from PREMIUM (D372SL00001), a European multicentre study. Methods: Ceftaroline MICs were determined by reference BMD and by Etest for 1242 MSSA and MRSA isolates collected between February and May 2012 from adult patients with community-acquired pneumonia or complicated skin and soft tissue infections; tests were performed across six European laboratories. Selected isolates with ceftaroline resistance in broth (MIC >1 mg/L) were retested in three central laboratories to confirm their behaviour. Results: Overall concordance…

Male0301 basic medicineCephalosporinPharmacologiemedicine.disease_causeCommunity-acquired pneumoniaPneumonia StaphylococcalCommunity-Acquired InfectionPharmacology (medical)Pathologie maladies infectieusesAged 80 and overMicrobial Sensitivity TestBroth microdilutionCeftalorine; Staphylococcus aureus; PREMIUM STUDY GROUPCeftalorineMiddle AgedAnti-Bacterial AgentsCommunity-Acquired InfectionsEuropeInfectious DiseasesStaphylococcus aureusStaphylococcus aureuStaphylococcal Skin InfectionsFemaleHumanAdultMicrobiology (medical)medicine.medical_specialtyStaphylococcus aureusAdolescentmedicine.drug_classCephalosporin030106 microbiologyPREMIUM STUDY GROUPMicrobial Sensitivity TestsStaphylococcal Skin InfectionMicrobiologyYoung Adult03 medical and health sciencesInternal medicineAnti-Bacterial AgentmedicineHumansEtestAgedPharmacologyAdult patientsbusiness.industrybiochemical phenomena metabolism and nutritionmedicine.diseasebacterial infections and mycosesMethicillin-resistant Staphylococcus aureusCephalosporinsMethicillin Susceptible Staphylococcus Aureusbusiness
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Daptomycin for children in clinical practice experience

2016

Data on daptomycin use in the pediatric setting are scanty. We conducted a multicenter, retrospective study on 46 children treated with intravenous daptomycin at a mean dosage of 7.0 mg/kg/d, for a median of 14 days. Three children had adverse events possibly related to daptomycin. The drug was overall well tolerated, even with prolonged treatment.

Male0301 basic medicinePediatricsmedicine.disease_causePediatrics0302 clinical medicinepolycyclic compounds030212 general & internal medicineChildChildrenMedicine (all)OsteomyelitisBacterial InfectionsPerinatology and Child HealthAnti-Bacterial AgentsClinical PracticeInfectious DiseasesChild PreschoolAdministration IntravenousFemalelipids (amino acids peptides and proteins)Children; Daptomycin; Methicillin-resistant Staphylococcus aureus; Osteomyelitis; Sepsis; Pediatrics Perinatology and Child Health; Medicine (all); Microbiology (medical); Infectious DiseasesMethicillin-resistant Staphylococcus aureusmedicine.drugMicrobiology (medical)medicine.medical_specialtyAdolescentDrug-Related Side Effects and Adverse Reactions030106 microbiologyChildren; Daptomycin; Methicillin-resistant Staphylococcus aureus; Osteomyelitis; Sepsis;Sepsis03 medical and health sciencesDaptomycinSepsismedicineHumansAdverse effectRetrospective Studiesbusiness.industryOsteomyelitisInfant NewbornInfantRetrospective cohort studybiochemical phenomena metabolism and nutritionbacterial infections and mycosesmedicine.diseaseMethicillin-resistant Staphylococcus aureuscarbohydrates (lipids)Pediatrics Perinatology and Child HealthDaptomycinbusinessProlonged treatment
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Time to blood culture positivity: An independent predictor of infective endocarditis and mortality in patients with Staphylococcus aureus bacteraemia

2019

International audience; Objectives - Time to blood culture positivity (TTP), a routinely available parameter in automated blood culture systems, may be a proxy for infectious burden in patients with bloodstream infections. We aimed to study the association between TTP and infective endocarditis (IE), or death, in patients with Staphylococcus aureus bacteraemia. Methods - VIRSTA is a multicenter prospective cohort study that included all adult patients with S. aureus bacteraemia in eight university hospitals in France (2009-2011). We analyzed data from four centers which collected data on TTP. Regression models were used to study the association between TTP and definite IE (Duke-Li criteria)…

MaleMethicillin-Resistant Staphylococcus aureus0301 basic medicineMicrobiology (medical)Staphylococcus aureusbacteraemiamedicine.medical_specialtyTime FactorsMultivariate analysis030106 microbiologyBacteremiaIndependent predictormedicine.disease_cause03 medical and health sciences0302 clinical medicineInterquartile rangehemic and lymphatic diseasesInternal medicinemedicineHumansBlood cultureProspective Studies030212 general & internal medicinetime to blood culture positivityProspective cohort study[SDV.MP] Life Sciences [q-bio]/Microbiology and ParasitologyAgedmedicine.diagnostic_testinfective endocarditisbusiness.industryEndocarditis BacterialGeneral MedicineMiddle AgedStaphylococcal Infectionsrespiratory systemmedicine.diseasemortality3. Good health[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyInfectious DiseasesQuartileBlood CultureStaphylococcus aureusInfective endocarditisFemalebusiness[SDV.MHEP]Life Sciences [q-bio]/Human health and pathologyClinical Microbiology and Infection
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Methicillin-resistant Staphylococcus aureus nasal colonization in a level III neonatal intensive care unit: Incidence and risk factors

2015

Objective: To describe epidemiologic features and identify risk factors for methicillin-resistant Staphylococcus aureus (MRSA) acquisition in a level III neonatal intensive care unit (NICU). Setting: A prospective, cohort study in a university-affiliated NICU with an infection control program including weekly nasal cultures of all neonates. Methods: Demographic, clinical, and microbiologic data were prospectively collected between June 2009 and June 2013. Molecular characterization of MRSA isolates was done by multilocus variable number tandem repeat fingerprinting, staphylococcal cassette chromosome mec typing, and on representative isolates by multilocus sequence typing and s. pa typing. …

MaleMethicillin-Resistant Staphylococcus aureusColonization pressurePediatricsmedicine.medical_specialtyNeonatal intensive care unitGenotypeEpidemiologyInfection controlInfectious DiseaseActive surveillancemedicine.disease_causeCohort StudiesHospitals UniversityRisk FactorsIntensive Care Units NeonatalEpidemiologymedicineInfection controlHumansTypingProspective StudiesMolecular EpidemiologyActive surveillance; Colonization pressure; Infection control; Infectious Diseases; Public Health Environmental and Occupational Health; Epidemiology; Health Policybusiness.industryIncidenceHealth PolicyInfant NewbornPublic Health Environmental and Occupational HealthStaphylococcal InfectionsMethicillin-resistant Staphylococcus aureusMolecular TypingVariable number tandem repeatNasal MucosaInfectious DiseasesStaphylococcus aureusCarrier StateMultilocus sequence typingFemalebusiness
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Molecular mechanisms of NET formation and degradation revealed by intravital imaging in the liver vasculature

2015

AbstractNeutrophil extracellular traps (NETs) composed of DNA decorated with histones and proteases trap and kill bacteria but also injure host tissue. Here we show that during a bloodstream infection with methicillin-resistant Staphylococcus aureus, the majority of bacteria are sequestered immediately by hepatic Kupffer cells, resulting in transient increases in liver enzymes, focal ischaemic areas and a robust neutrophil infiltration into the liver. The neutrophils release NETs into the liver vasculature, which remain anchored to the vascular wall via von Willebrand factor and reveal significant neutrophil elastase (NE) proteolytic activity. Importantly, DNase although very effective at D…

MaleMethicillin-Resistant Staphylococcus aureusExtracellular TrapsProteasesHydrolasesKupffer CellsGeneral Physics and AstronomyBacteremiaBiologyHepatic Veinsmedicine.disease_causeExtracellular TrapsGeneral Biochemistry Genetics and Molecular BiologyHistonesMiceHepatic ArteryVon Willebrand factorProtein-Arginine Deiminase Type 4von Willebrand FactormedicineAnimalsMice KnockoutMultidisciplinaryDeoxyribonucleasesGeneral ChemistryNeutrophil extracellular trapsStaphylococcal Infectionsmedicine.disease3. Good healthCell biologyHistoneLiverNeutrophil InfiltrationStaphylococcus aureusNeutrophil elastaseImmunologybiology.proteinLeukocyte ElastaseInfiltration (medical)
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