Search results for "Mine"
showing 10 items of 20410 documents
Arabidopsis thaliana nicotianamine synthase 4 is required for proper response to iron deficiency and to cadmium exposure.
2013
International audience; The nicotianamine synthase (NAS) enzymes catalyze the formation of nicotianamine (NA), a non-proteinogenic amino acid involved in iron homeostasis. We undertook the functional characterization of AtNAS4, the fourth member of the Arabidopsis thaliana NAS gene family. A mutant carrying a T-DNA insertion in AtNAS4 (atnas4), as well as lines overexpressing AtNAS4 both in the atnas4 and the wild-type genetic backgrounds, were used to decipher the role of AtNAS4 in NA synthesis, iron homeostasis and the plant response to iron deficiency or cadmium supply. We showed that AtNAS4 is an important source for NA. Whereas atnas4 had normal growth in iron-sufficient medium, it dis…
Characterization of the biodegradation, bioremediation and detoxification capacity of a bacterial consortium able to degrade the fungicide thiabendaz…
2017
Thiabendazole (TBZ) is a persistent fungicide used in the post-harvest treatment of fruits. Its application results in the production of contaminated effluents which should be treated before their environmental discharge. In the absence of efficient treatment methods in place, biological systems based on microbial inocula with specialized degrading capacities against TBZ could be a feasible treatment approach. Only recently the first bacterial consortium able to rapidly transform TBZ was isolated. This study aimed to characterize its biodegradation, bioremediation and detoxification potential. The capacity of the consortium to mineralize 14C-benzyl-ring labelled TBZ was initially assessed. …
Biochemical Quality of Crop Residues and Carbon and Nitrogen Mineralization Kinetics under Nonlimiting Nitrogen Conditions
2000
International audience; Statistical relationships were established between the fate of C and N from 47 types of crop residues and their biochemical characteristics during a soil incubation at 15°C. The incubations were carried out under nonlimiting N in order to differentiate the effects of biochemical characteristics of residues from those of soil N availability. Depending on the residue, the apparent mineralization of residue C after 168 d varied from 330 to 670 g kg−1 of added C. Mineralization kinetics were described using a two-compartment decomposition model that decomposes according to first-order kinetics. Amounts of C mineralized after 7 d and the decomposition rate coefficient of …
Polyamine metabolism during seedling development in rice
1994
The main free amines identified during growth and development of rice seedlings were agmatine, putrescine, spermidine, diaminopropane and tyramine. Amine composition differed according to tissue and stages of development. Conjugated amines were only found in roots. We present evidence that arginine decarboxylase (ADC) regulates putrescine during the development of rice seedlings. When ADC action was blocked by DFMA (α-DL-difluoromethylarginine, a specific irreversible inhibitor of ADC), polyamine titers and seedling development were diminished; when agmatine or putrescine was added, normal polyamine titers and growth were restored. The effects of DFMA were concentration dependent. DFMO (α-D…
Polyamines and related enzymes in rice seeds differing in germination potential
1994
In ungerminated rice seeds, (Japonica rice variety, CV Tapei 309), the content of free amines (putrescine, spermidine, spermine, tyramine) was higher in seed lots having a low germination frequency compared to those with high germination potential. Conversely, amine conjugates (di-feruloylputrescine, di-feruloylspermidine, diferuloyldiaminopropane and feruloyltyramine) decreased with loss of viability. Thus, these compounds appeared to constitute biochemical markers of seed viability. In seeds with high germination potential, conjugates decreased drastically during germination, with an early and rapid increase in free amines (putrescine, spermidine, tyramine). Arginine decarboxylase (ADC) a…
Lipid composition of the vacuolar membrane of Acer pseudoplatanus cultured cells
1993
Tonoplast was prepared by osmotic lysis of a pure vacuolar fraction isolated from protoplasts derived from Acer pseudoplatanus cultured cells. After their extraction, neutral and polar lipids were separated by a thin layer chromatography. Phospholipids, glycolipids and neutral lipids represented 44.5%, 39.1% and 16.4% of total lipids, respectively. Sterols (glycosylated plus non-glycosylated forms) constituted 30.8% of total lipids; 75% of sterols were glycosylated. The most prominent lipids were phosphatidylethanolamine (20.8%), phosphatidylcholine (13.5%), ceramide monohexoside (12.8%), steryl glycoside (12.2%) and acylated steryl glycoside (10.9%). Glucose was the only sugar released by …
Use of chlorophyll fluorescence induction kinetics to study translocation and detoxication of DCMU-type herbicides in plant leaves
1984
Transient levels of the fluorescence induction rise were used to quantify partial photosynthesis inhibition by DCMU -type herbicides in whole leaves. Assays in different crop or weed species showed a good accuracy in measurements (generally, variation was lower than 5%). This technique was applied to the problem of varietal selectivity of wheat towards chlortoluron.
Wood-decaying fungi in old-growth boreal forest fragments: extinctions and colonizations over 20 years
2021
According to ecology theory, isolated habitat fragments cannot maintain populations of specialized species. Yet, empirical evidence based on monitoring of the same fragments over time is still limited. We studied the colonizationâextinction dynamics of eight wood-decaying fungal species in 16 old-growth forest fragments (<14 ha) over a 20-year period (1997â2017). We observed 19 extinctions and 5 colonizations; yet, the distribution of extinctions and colonizations did not differ from the one expected by chance for any of the species. Twenty-six percent of the extinctions took place in two natural fragments amid large forestâpeatland complexes. (Romell) Bourdot and Galzin decreased …
Effects of magnesium deprivation on development and biomineralization in the sea urchin Arbacia lixula
2019
Echinoderms have an extensive endoskeleton composed of magnesian calcite and occluded matrix proteins. As biomineralization in sea urchin larvae is sensitive to the Magnesium:Calcium ratio of seawater, we investigated the effects of magnesium deprivation on development and skeletogenesis in the sea urchin Arbacia lixula. We focused on the localization of the skeletogenic cells (primary mesenchyme cells) and the spatial expression of associated genes. Embryos reared in Mg-free seawater exhibited developmental delay from 6-h post-fertilization and at 24 h embryos showed complete lack of biomineral formation. Larvae (48–72 h) exhibited severe skeleton malformations. Fluorescent labelling revea…
Negative associations between parasite avoidance, resistance and tolerance predict host health in salmonid fish populations
2020
Genetic variation in defence against parasite infections is fundamental for host–parasite evolution. The overall level of defence of a host individual or population includes mechanisms that reduce parasite exposure (avoidance), establishment (resistance) or pathogenicity (tolerance). However, how these traits operate and evolve in concert is not well understood. Here, we investigated genetic variation in and associations between avoidance, resistance and tolerance in a natural host–parasite system. Replicated populations of Atlantic salmon ( Salmo salar ) and sea trout (an anadromous form of brown trout, Salmo trutta ) were raised under common garden conditions and infected with the eye fl…