Search results for "Multiplex"
showing 10 items of 337 documents
Power allocation optimization in OFDM-based cognitive radios based on sensing information
2011
Owing to the non-zero probability of the missed detection and false alarm of active primary transmission, a certain degree of performance degradation of the primary user (PU) from cognitive radio users (CRs) is unavoidable. In this paper, we consider OFDM-based communication systems and present efficient algorithms to maximize the total rate of the CR by optimizing jointly both the detection operation and the power allocation, taking into account the influence of the probabilities of missed detection and false alarm, namely, the sensing accuracy. The optimization problem can be formulated as a two-variable non-convex problem, which can be solved approximately by using an alternating directi…
A polarimetric sensor based on nanoporous free standing membranes
2012
A polarimetric sensor with state of the art sensitivity is developed using free standing porous silicon membranes. The use of an optimized etching receipt greatly reduces the pore roughness. Depolarization factors are thus limited and material birefringence is increased. Free standing membranes are fabricated in n-type substrates and characterized both from the optical and structural point of view. The proposed approach is fully CMOS compatible and can therefore pave the way to the development of cheap microarray that exploits multiplexing capabilities while keeping the amount of analyte required by the analysis down to the microliter level.
Rapid microarray-based typing of forensic SNPs
2006
The single base extension-tag array (SBE-Tag Array) method is carried out on glass slides and combines the specificity of minisequencing for SNP typing with the high throughput capacity of microarrays. Following multiplex PCR, a single tube SBE reaction is carried out, and the fluorescent labelled extension products are hybridized to the complementary DNA sequence tag (cTag) immobilized on a glass slide for locus-specific laser scan analysis. The aim is to prove and optimise the conventional microarray reaction on accuracy and efficiency for forensic applications. © 2005 Elsevier B.V. All rights reserved.
A multiplex PCR for the detection of Vibrio vulnificus hazardous to human and/or animal health from seafood
2022
Vibrio vulnificus is a zoonotic pathogen linked to aquaculture that is spreading due to climate change. The pathogen can be transmitted to humans and animals by ingestion of raw shellfish or seafood feed, respectively. The aim of this work was to design and test a new procedure to detect V. vulnificus hazardous to human and/or animal health in food/feed samples. For this purpose, we combined a pre-enrichment step with multiplex PCR using primers for the species and for human and animal virulence markers. In vitro assays with mixed DNA from different Vibrio species and Vibrio cultures showed that the new protocol was 100 % specific with a detection limit of 10 cfu/mL. The protocol was succes…
Comparison of the performance of 2 commercial multiplex PCR platforms for detection of respiratory viruses in upper and lower tract respiratory speci…
2015
The performance of the CLART® PneumoVir system with that of the Luminex xTAG RVP Fast v1 assay for detection of most common respiratory viruses in upper and lower tract respiratory specimens (n = 183) from unique patients with influenza-like syndrome or lower tract respiratory infection. Nested PCR coupled to automated sequencing was used for resolution of discrepancies. Fully concordant results were obtained for a total of 122 specimens, whereas 56 specimens gave partially (n = 21) or fully discordant (n = 35) results (Kappa coefficient, 0.62). The overall specificity of the Luminex xTAG RVP Fast v1 assay was slightly higher than that of the CLART® PneumoVir assay for human bocavirus, infl…
VanB-VanC1 Enterococcus gallinarum, Italy
2005
To the Editor: We report detecting a vanB determinant in Enterococcus gallinarum in poultry in Italy. High-level vanA-mediated glycopeptide resistance has been described for E. gallinarum and E. casseliflavus (1–4), and vanB-mediated vancomycin resistance has been frequently described for E. faecalis and E. faecium. However, vanB-mediated resistance in isolates of E. gallinarum has been described only in sporadic nosocomial cases of infection or colonization (5,6). In January 2005, a study of contamination by foodborne organisms in slaughtered broiler carcasses was conducted in Sicily. To detect glycopeptide-resistant enterococci (GRE), each carcass was placed in a bag with 100 mL sterile b…
The use of multiplex PCR to detect and differentiate food- and beverage-associated microorganisms: a review.
2007
Regarding food safety, rapid detection of microbial species is crucial to develop effective preventive and/or adjustment measures. Classical methods for determining the presence of certain species are time-consuming and labor-intensive, hence, molecular methods, which offer speed, sensitivity and specificity, have been developed to address this problem. Multiplex PCR (MPCR) is widely applied in the various fields of microbiology for the rapid differentiation of microbial species without compromising accuracy. This paper describes the method and reports on the state-of-the-art application of this technique to the identification of microorganisms vehiculated with foods and beverages. The iden…
Comparison of the BD Directigen Flu A+B Kit and the Abbott TestPack RSV with a multiplex RT-PCR ELISA for rapid detection of influenza viruses and re…
2005
ABSTRACTThe Directigen Flu A+B enzyme immunoassay and the Abbott TestPack RSV enzyme immunoassay were each compared with a multiplex RT-PCR ELISA by testing 635 nasopharyngeal aspirates collected from children aged < 16 years who had been hospitalised with acute respiratory tract infection during the epidemic season 2002–2003. In this study, the sensitivity of the Directigen Flu A+B assay was unacceptably low (29.3% and 10.0%, respectively) for the detection of influenza A and B viruses. The sensitivity of the Abbott TestPack RSV assay (77.4%) was acceptable and in agreement with the multiplex RT-PCR ELISA.
Micro-optical components for parallel optical networks
2002
Key components for parallel optical links are fabricated cost-effectively using LIGA technology. Multifiber ferrules and mechanical splices, 1/spl times/2 and 4/spl times/4 single-mode matrix switches and micro lens arrays with passive alignment structures are presented.
Time-multiplexing Integral Microscopy
2014
Conventional microscopes do not capture the 3D information of thick specimens. In order to avoid this limitation Integral Microscopy was proposed. An integral microscope is implemented by inserting a microlens array between the microscope objective and the camera sensor. Although this device captures multiperspective information of the specimen, the small number of microlenses limits the lateral resolution. In this paper we propose to improve the resolution by time multiplexing. Specifically, we propose an electrically addressable device that permits to obtain three sheared versions of the microscopic plenoptic map. Digital processing algorithm applied to the maps provides images with resol…