Search results for "Musculoskeletal"

Long-Term Potentiation in the Recurrent Inhibitory Circuit of the Dentate Gyrus

1988

The question of whether long-term potentiation occurs in the inhibitory circuits of the hippocampus remains controversial. Buszaki and Eidelberg (1982), recording extracellularly from putative interneurones (basket cells) in the dentate gyrus and area CAl of the anaesthetized rat, found a prolonged increase in probability of cell firing to afferent stimulation after high-frequency stimulation of Schaffer-commissural fibres, and concluded that LTP occurs at excitatory feedforward synapses onto interneurones. Similarly, Kairis et al (1987) have presented field potential evidence for LTP in feedforward synapses onto inhibitory neurones in the dentate gyrus of the anaesthetized rat. In the hipp…

Bone damage after chemotherapy for lymphoma: a real-world experience

2021

AbstractBackgroundDespite recent improvements in survival due to advances in treatment, the quality of life of patients with lymphoma may be compromised by the long-term complications of chemotherapy and steroid therapy. Among these, a potentially relevant problem is bone loss and the development of fragility fractures.AimTo provide further evidence of clinical or subclinical skeletal complications in correlation with biological variables and markers of bone disease in patients with complete response to therapy.MethodA cross-sectional observational study was conducted on subjects diagnosed with lymphoma with subsequent antineoplastic treatment, disease status after therapy defined as comple…

The Relationship Between Attachment and Psychopathy: A Study with a Sample of Violent Offenders

2014

This study used a mixed quantitative-qualitative methodology to investigate the relationship between attachment and psychopathy. The Psychopathy Checklist- Revised (PCL-R; Hare 2003) was administered to 139 Italian offenders who were convicted of violent crimes (murder, rape, child sexual abuse, armed robbery, assault causing bodily harm). First, we explored whether the two PCL-R items theoretically denoting devaluation of attachment bonds (promiscuous sexual behavior and many marital relationships) were able to predict PCL-R total, factor and facet scores. Subse- quently, we analyzed the transcripts of the 10 participants who obtained the highest PCL-R scores in the sample, assessing their…

Down-regulation of transcription factors AP-1, Sp-1, and NF-kappa B precedes myocyte differentiation.

1996

Terminal differentiation of myocytes involves withdrawal from the cell cycle, induction of myogenin expression, and finally formation of myotubes. To study the factors that regulate the initial phase of muscle differentiation, we analyzed the binding activities of transcription factors AP-1, Sp-1, and NF-kappa B in L6, C2C12, and rhabdomyosarcoma BA-Han-1C cells. Temporal changes in transcription factor binding activities were compared to the activation of myogenin promoter-driven CAT reporter gene and the expression level of myogenin, a master gene of myogenic differentiation. We observed a prominent decrease in the nuclear binding activities of AP-1, Sp-1, and NF-kappa B already 12 to 24 …

Biologically Active Triterpene Saponins from Callus Tissue of Polygala amarella

1999

A new bioactive saponin (1), together with a known saponin (polygalasaponin XXVIII) has been isolated from the callus tissue culture of Polygala amarella. Based on spectroscopic data, especially direct and long-range heteronuclear 2D NMR analysis and on chemical transformations, the structure of 1 was elucidated as 3-O-beta-D-glucopyranosyl presenegenin-28-O-beta-D-galactopyranosyl-(1 --> 3)-beta-D-xylopyranosyl-(1 --> 4)-alpha-L-rhamnopyranosyl-(1 --> 2)-[beta-D-glucopyranosyl-(1 --> 3)]-beta-D-fucopyranoside. Both saponins showed significant immunological properties based on the enhancement of granulocyte phagocytosis in vitro.

Vascular resistance arm of the baroreflex: methodology and comparison with the cardiac chronotropic arm.

2020

Baroreflex response consists of cardiac chronotropic (effect on heart rate), cardiac inotropic (on contractility), venous (on venous return) and vascular (on vascular resistance) arms. Because of its measurement simplicity, cardiac chronotropic arm is most often analysed. The aim was to introduce a method to assess vascular baroreflex arm, and to characterize its changes during stress. We evaluated the effect of orthostasis and mental arithmetics (MA) in 39 (22 female, median age: 18.7 yrs.) and 36 (21 female, 19.2 yrs.) healthy volunteers, respectively. We recorded systolic and mean blood pressure (SBP and MBP) by volume-clamp method and R-R interval (RR) by ECG. Cardiac output (CO) was re…

Characterization of the interaction between Actinin-Associated LIM Protein (ALP) and the rod domain of α-actinin

2009

Abstract Background The PDZ-LIM proteins are a family of signalling adaptors that interact with the actin cross-linking protein, α-actinin, via their PDZ domains or via internal regions between the PDZ and LIM domains. Three of the PDZ-LIM proteins have a conserved 26-residue ZM motif in the internal region, but the structure of the internal region is unknown. Results In this study, using circular dichroism and nuclear magnetic resonance (NMR), we showed that the ALP internal region (residues 107–273) was largely unfolded in solution, but was able to interact with the α-actinin rod domain in vitro, and to co-localize with α-actinin on stress fibres in vivo. NMR analysis revealed that the ti…

2013

The amyloid-β42 (Aβ42) peptide plays a crucial role in the pathogenesis of Alzheimer’s disease (AD), the most common neurodegenerative disorder affecting the elderly. Over the past years, several approaches and compounds developed for the treatment of AD have failed in clinical studies, likely in part due to their low penetration of the blood–brain barrier (BBB). Since nanotechnology-based strategies offer new possibilities for the delivery of drugs to the brain, this technique is studied intensively for the treatment of AD and other neurological disorders. The Aβ42 lowering drug flurbiprofen was embedded in polylactide (PLA) nanoparticles by emulsification-diffusion technique and their pot…

Blocking NMDA-receptors in the pigeon's "prefrontal" caudal nidopallium impairs appetitive extinction learning in a sign-tracking paradigm

2015

Extinction learning provides the ability to flexibly adapt to new contingencies by learning to inhibit previously acquired associations in a context-dependent manner. The neural networks underlying extinction learning were mostly studied in rodents using fear extinction paradigms. To uncover invariant properties of the neural basis of extinction learning, we employ pigeons as a model system. Since the prefrontal cortex of mammals is a key structure for extinction learning, we assessed the role of N-methyl-D-aspartate receptors (NMDARs) in the nidopallium caudolaterale, the avian functional equivalent of mammalian prefrontal cortex. Since NMDARs in prefrontal cortex have been shown to be rel…

Novel Biodegradable Composite of Calcium Phosphate Cement and the Collagen I Mimetic P-15 for Pedicle Screw Augmentation in Osteoporotic Bone

2021

Osteoporotic vertebral fractures often necessitate fusion surgery, with high rates of implant failure. We present a novel bioactive composite of calcium phosphate cement (CPC) and the collagen I mimetic P-15 for pedicle screw augmentation in osteoporotic bone. Methods involved expression analysis of osteogenesis-related genes during osteoblastic differentiation by RT-PCR and immunostaining of osteopontin and Ca2+ deposits. Untreated and decalcified sheep vertebrae were utilized for linear pullout testing of pedicle screws. Bone mineral density (BMD) was measured using dual-energy X-ray absorptiometry (DEXA). Expression of ALPI II (p &lt