Search results for "Nitrogen"
showing 10 items of 1200 documents
PEMBEKUAN SEMEN LELE DUMBO (Clarias Gariepinus BURCHELL 1822) SEBAGAI MODEL KRIOPRESERVASI SEMEN IKAN
2010
<p><em>Effect of four types of diluents</em><em>s</em><em> and four concentration of DMSO (5%, 10%, 15% and 20%) against the motility of African catfish sperm were evaluated after storage at freezing temperatures. </em><em>The steps in preparation the 16 treatments combination of the diluents</em><em> </em><em>are </em><em>preparation of diluents, mixing </em><em>diluents </em><em>with DMSO, packing </em><em>of semen </em><em>in</em><em>to</em><em> 0.3-ml straw, equilibration of </em><em>semen </em><em>at 4 °C for…
A rapid and cost-efficient DMSO-based method for isolating DNA from cultured lichen photobionts
2010
We have developed a simple and fast procedure for the purification of PCR-quality DNA from cultured lichen photobionts. This new one-step method uses the solvent dimethyl sulfoxide (DMSO) combined with heat treatment to chemically breakdown algal and plant tissues. The DMSO-DNA extracts may be directly precipitated and purified by standard techniques in a total time of approximately 30 min. Compared to other DNA extraction protocols, the DMSO-based method suppresses the need for liquid nitrogen, any extraction buffer, grinding, phenol, and long incubation or centrifugation steps, thereby considerably reducing the possibility of contamination. In addition, minimal amounts of starting materia…
A microscale Kjeldahl nitrogen determination for environmental waters.
2008
A microscale Kjeldahl system has been designed which has been homemade reduced. The digestor unit of a macroKjeldahl system and a modified glassware microdistillation unit have been used. The optimal conditions for digestion and distillation have been established for ammonium and methylamine as model compound of organic amino nitrogen. The optimised procedure has been applied to the determination of Kjeldahl nitrogen in water samples. Recoveries near 100% and good precision have been achieved. This procedure combines nitrogen miniaturized system and the use of a selective ion electrode for ammonia detection. The analysis time was reduced 4.8 times and the analysis cost 6.6 times compared wi…
Isolation and concentration of organophosphorus pesticides from water using a c18 reversed phase
1989
Abstract A simple, rapid and effective method for the extraction and enrichment of organophosphorus pesticides based on the use of Sep-Pak C 18 cartridges was studied as alternative method to those based on extraction with organic solvents. The influence of the elution solvent, pH, salinity and volume of water filtered was studied for ten organophosphorus pesticides. The pesticides were determined by gas chromatography with a BP-1 capillary column and a thermionic detector. Recoveries at the 100 and 200 ng/1 spiking levels were greater than 85%, except for disulfoton.
Insights into the annotated genome sequence of Methanoculleus bourgensis MS2(T), related to dominant methanogens in biogas-producing plants
2014
The final step of the biogas production process, the methanogenesis, is frequently dominated by members of the genus Methanoculleus. In particular, the species Methanoculleus bourgensis was identified to play a role in different biogas reactor systems. The genome of the type strain M. bourgensis MS2(T), originally isolated from a sewage sludge digestor, was completely sequenced to analyze putative adaptive genome features conferring competitiveness within biogas reactor environments to the strain. Sequencing and assembly of the M. bourgensis MS2(T) genome yielded a chromosome with a size of 2,789,773 bp. Comparative analysis of M. bourgensis MS2(T) and Methanoculleus marisnigri JR1 revealed…
mdm2 mRNA expression is associated with survival in ovarian cancer
1997
Expression of mdm-2 mRNA was measured in 90 ovarian-cancer tissue specimens using the S1 nuclease assay, to investigate a possible association between MDM2 expression and prognosis. mdm-2 mRNA expression was an independent prognostic factor for patients with primary ovarian cancer, FIGO (International Federation of Gynecology and Obstetrics) stages III and IV (n = 57), who all received chemotherapy with carboplatin or cisplatin and cyclophosphamide. Median survival time for patients (FIGO stages III and IV) with no detectable expression of mdm-2 mRNA (n = 14) was 171 days, as compared with 839 days for patients (n = 43) with detectable mdm-2 mRNA (p = 0.0194; log-rank test). However, no ass…
Synthesis, spectroscopic characterization and antiproliferative activity of two platinum(II) complexes containing N-donor heterocycles
2014
Abstract Novel mononuclear complexes of Pt(II), cis -[PtCl 2 (DMSO)HL]·2DMSO ( 1 ), where HL = 7-amino-2-(methylthio)[1,2,4]triazolo[1,5- a ]pyrimidine-6-carboxylic acid and Pt(bdt)Cl 2 ( 2 ), where bdt = [2,4-bis(5,6-diphenyl-1,2,4-triazin-3yl)-pyridine] have been synthesized and characterized by elemental analysis, IR and 1 H NMR spectroscopy and X-ray crystallography diffraction analyses. The molecular structure of ( 1 ) shows that Pt(II) ion has a square planar geometry with N(3) bonded heterocycle ligand, two cis chloride anions and S-bonded dimethylsulfoxide. The antiproliferative activity of complexes ( 1 ) and ( 2 ) has been tested in vitro against HepG2 human hepatoma cells and non…
Combined Carboplatin plus Ifosfamide and Cisplatin in Patients with Advanced Ovarian Carcinoma. A Phase I–II Study
1998
Abstract Because of the relative lack of overlapping toxicity, carboplatin (PPL) and cisplatin (CDDP) can be easily combined for treatment of ovarian cancer to increase total platinum dose intensity. Ifosfamide (IFO), one of the most effective single agents in ovarian cancer, has a low hematological toxicity when administered in continuous infusion. From January 1991 to December 1993, 34 patients with advanced ovarian cancer, previously untreated with chemo- or radiotherapy, were enrolled in a phase I–II study with the aim of determining the maximum tolerated dose (MTD) of CDDP (on day 8 of a 28-day cycle) in combination with PPL (300 mg/m 2 on day 1) and IFO (4,000 mg/m 2 /24 h by continuo…
Wastewaters from citrus processing industry as natural biostimulants for soil microbial community
2020
Abstract Citrus fruit processing wastewaters (CWWs), being rich in organic matter, may be a valuable resource for agricultural irrigation and, possibly, for the improvement of soil organic carbon (TOC). This issue is becoming crucial for soils of arid and semiarid environments increasingly experiencing water scarcity and continuous decline of TOC towards levels insufficient to sustain crop production. However, before using CWWs in agriculture their effects on the soil living component have to be clarified. Therefore, in this study we assessed the impact of CWWs on soil chemical and biochemical properties. Under laboratory conditions, lemon, orange and tangerine wastewaters were separately a…
Saccharomyces cerevisiae signature genes for predicting nitrogen deficiency during alcoholic fermentation
2007
Genome-wide analysis of the wine yeast strain Saccharomyces cerevisiae PYCC4072 identified 36 genes highly expressed under conditions of low or absent nitrogen in comparison with a nitrogen-replete condition. Reverse transcription-PCR analysis for four of these transcripts with this strain and its validation with another wine yeast strain underlines the usefulness of these signature genes for predicting nitrogen deficiency and therefore the diagnosis of wine stuck/sluggish fermentations.