Search results for "ON-CHIP"
showing 10 items of 20 documents
Contrasting topologies for regular interconnection networks under the constraints of nanoscale silicon technology
2010
Nowadays, system designers have adopted Networks-on-Chip as communication infrastructure of general-purpose tile-based Multi-Processor System-on-Chip (MPSoC). Such decision implies that a certain topology has to be selected to efficiently interconnect many cores on the chip. To ease such a choice, the networking literature offers a plethora of works about topology analysis and characterization for the off-chip domain. However, theoretical parameters and many intuitive assumptions of such off-chip networks do not necessarily hold when a topology is laid out on a 2D silicon surface. This is due to the distinctive features of silicon technology design pitfalls. This work is a first milestone t…
Nut1/Hos1 and Sas2/Rpd3 control the H3 acetylation of two different sets of osmotic stress-induced genes
2019
Epigenetic information is able to interact with the cellular environment and could be especially useful for reprograming gene expression in response to a physiological perturbation. In fact the genes induced or repressed by osmotic stress undergo significant changes in terms of the levels of various histone modifications, especially in the acetylation levels of histone H3. Exposing yeast to high osmolarity results in the activation of stress-activated protein kinase Hog1, which plays a central role in gene expression control. We evaluated the connection between the presence of Hog1 and changes in histone H3 acetylation in stress-regulated genes. We found a parallel increase in the acetylati…
Chromatin Immunoprecipitation Assay to Identify Genomic Binding Sites of Regulatory Factors
2016
DNA-protein interactions are vital to fundamental cellular events including transcription, replication, DNA repair, and recombination. Thus, their study holds the key to our understanding of mechanisms underlying normal development and homeostasis as well as disease. Transcriptional regulation is a highly complex process that involves recruitment of numerous factors resulting in formation of multi-protein complexes at gene promoters to regulate gene expression. The studied proteins can be, for example, transcription factors, epigenetic regulators, co-activators, co-repressors, or ligand-activated nuclear receptors as estrogen receptor-α (ERα) bound either directly to the DNA or indirectly b…
Systematic gene overexpression in Candida albicans identifies a regulator of early adaptation to the mammalian gut.
2018
International audience; Candida albicans is part of the human gastrointestinal (GI) microbiota. To better understand how C. albicans efficiently establishes GI colonisation, we competitively challenged growth of 572 signature-tagged strains (~10% genome coverage), each conditionally overexpressing a single gene, in the murine gut. We identified CRZ2, a transcription factor whose overexpression and deletion respectively increased and decreased early GI colonisation. Using clues from genome-wide expression and gene-set enrichment analyses, we found that the optimal activity of Crz2p occurs under hypoxia at 37°C, as evidenced by both phenotypic and transcriptomic analyses following CRZ2 geneti…
Role of p27Kip1 as a transcriptional regulator
2018
The protein p27Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase (Cdk) inhibitors. It interacts with both the catalytic and the regulatory subunit (cyclin) and introduces a region into the catalytic cleave of the Cdk inducing its inactivation. Its inhibitory capacity can be modulated by specific tyrosine phosphorylations. p27Kip1 also behaves as a transcriptional regulator. It associates with specific chromatin domains through different transcription factors. ChIP on chip, ChIP-seq and expression microarray analysis allowed the identification of the transcriptional programs regulated by p27Kip1. Thus, important cellular functions as cell division cycle, respiration, RNA proc…
sevenC
2018
Chromatin looping is an essential feature of eukaryotic genomes and can bring regulatory sequences, such as enhancers or transcription factor binding sites, in the close physical proximity of regulated target genes. This package uses protein binding signals from ChIP-seq and sequence motif information to predict chromatin looping events.
Moving Learning Machine Towards Fast Real-Time Applications: A High-Speed FPGA-based Implementation of the OS-ELM Training Algorithm
2018
Currently, there are some emerging online learning applications handling data streams in real-time. The On-line Sequential Extreme Learning Machine (OS-ELM) has been successfully used in real-time condition prediction applications because of its good generalization performance at an extreme learning speed, but the number of trainings by a second (training frequency) achieved in these continuous learning applications has to be further reduced. This paper proposes a performance-optimized implementation of the OS-ELM training algorithm when it is applied to real-time applications. In this case, the natural way of feeding the training of the neural network is one-by-one, i.e., training the neur…
On the impact of within-die process variation in GALS-Based NoC Performance
2012
[EN] Current integration scales allow designing chip multiprocessors (CMP), where cores are interconnected by means of a network-on-chip (NoC). Unfortunately, the small feature size of current integration scales causes some unpredictability in manufactured devices because of process variation. In NoCs, variability may affect links and routers causing them not to match the parameters established at design time. In this paper, we first analyze the way that manufacturing deviations affect the components of a NoC by applying a new comprehensive and detailed within-die variability model to 200 instances of an 8¿8 mesh NoC synthesized using 45 nm technology. Later, we show that GALS-based NoCs pr…
A chromatin-associated protein from pea seeds preferentially binds histones H3 and H4
2002
Pisum sativum p16 is a protein present in the chromatin of ungerminated embryonic axes. The purification of p16 and the isolation of a cDNA clone of psp54, the gene encoding its precursor have been recently reported [Castillo, J., Rodrigo, M. I., Marquez, J. A., Zuniga, A and Franco, L. (2000) Eur. J. Biochem.267, 2156-2165]. In the present paper, we present data showing that p16 is a nuclear protein. First, after subcellular fractionation, p16 was clearly found in nuclei, although the protein is also present in other organelles. Immunocytochemical methods also confirm the above results. p16 seems to be firmly anchored to chromatin, as only extensive DNase I digestion of nuclei allows its r…
Oil-in-Water fL Droplets by Interfacial Spontaneous Fragmentation and Their Electrical Characterization
2019
Inkjet printing is here employed for the first time as a method to produce femtoliter-scale oil droplets dispersed in water. In particular, picoliter-scale fluorinated oil (FC40) droplets are printed in the presence of perfluoro-1-octanol surfactant at a velocity higher than 5 m/s. Femtoliter-scale oil droplets in water are spontaneously formed through a fragmentation process at the water/air interface using minute amounts of nonionic surfactant (down to 0.003% v/v of Tween 80). This fragmentation occurs by a Plateau-Rayleigh mechanism at a moderately high Weber number (10(1)). A microfluidic chip with integrated microelectrodes allows droplets characterization in terms of number and diamet…