Search results for "PLIF"

Innovation in the regeneratio of the historic centers in Sicily: Barcellona Pozzo di Gotto (ME)

2012

In-Field LAMP Detection of Flavescence Dorée Phytoplasma in Crude Extracts of the Scaphoideus titanus Vector

2022

One of the most destructive diseases affecting grapevine in Europe is caused by Flavescence Dorée phytoplasma (FDp), which belongs to the 16Sr-V group and is a European Union quarantine pathogen. Although many molecular techniques such as loop-mediated isothermal amplification (LAMP) are widely used for the rapid detection of FDp in infected grapevine plants, there is no developed isothermal amplification assay for FDp detection in the insect vectors that are fundamental for the spread of the disease. For this reason, a simple in-field real-time LAMP protocol was optimized and developed for the specific detection of FDp in the insect vector Scaphoideus titanus. The LAMP assay was optimized …

Aikuisten S2-oppijoiden kielenoppimisen kokemuksia selkomukautetun kaunokirjallisuuden parissa

2020

Artikkeli käsittelee oppimisen kokemuksia selkomukautetun kaunokirjallisuuden parissa kotoutumiskoulutuksen lukupiirissä. Aineistona ovat lukupiirien 24 osallistujan haastattelut. Haastattelut on tehty osallistujien toisella kielellä tai lingua franca -englannilla, ja niitä lähestytään fenomenologista ajattelutapaa hyödyntäen kertomuksina oppimisen kokemuksista. Kokemukset vaihtelevat yksilöllisesti, ja tärkeimmiksi merkityskokonaisuuksiksi nousevat sanat, kielen rakenteet ja kielenkäyttö. Kokemusten yksilöllisyyttä voidaan selittää sosiokulttuurisesta teoriasta käsin: vaikka selkomukautettu kaunokirjallisuus tarjoaa lukijalleen runsaasti kielellisiä affordansseja, tarjoumia, kukin lukija t…

Benefits and Drawbacks of A High Frequency Gan Zvzcps Converter

2017

This paper presents the benefits and drawbacks of replacing the traditional Si Mosfets transistors with enhancement mode GaN transistors in a Half-Bridge Zero Voltage and Zero Current Switching Power Switching (ZVZCPS) converter. This type of converters is usually used as Electronic Power Converters (EPC) for telecommunication satellites travelling-wave tube amplifiers (TWTAs). In this study, firstly the converter is theoretically analysed, obtaining its operation, losses and efficiency equations. From these equations, optimizations maps based on the main system parameters are obtained. These optimization maps are the key to quantify the potential benefits of GaN transistors in this type of…

Anàlisi contrastiva dels marcadors de reformulació i exemplificació

2001

The aim of this paper is to present a contrastive analysis of reformulation and exemplification markers in Catalan and Spanish, on the one hand, and in English, on the other. The study is based on a corpus of expository prose (mainly academic writing). The analysis shows coincidences regarding the sources of the connectives, but also differences relating to the variety of forms and the preference of fixed or variable connectives. The differences identified in the grammar of Catalan and Spanish vs. that of English will be associated with two distinct styles for building expository texts.

Multiplex Amplicon Quantification (MAQ), a fast and efficient method for the simultaneous detection of copy number alterations in neuroblastoma

2010

Abstract Background Cancer genomes display characteristic patterns of chromosomal imbalances, often with diagnostic and prognostic relevance. Therefore assays for genome-wide copy number screening and simultaneous detection of copy number alterations in specific chromosomal regions are of increasing importance in the diagnostic work-up of tumors. Results We tested the performance of Multiplex Amplicon Quantification, a newly developed low-cost, closed-tube and high-throughput PCR-based technique for detection of copy number alterations in regions with prognostic relevance for neuroblastoma. Comparison with array CGH and the established Multiplex Ligation-dependent Probe Amplification method…

Data from: Successive invasion-mediated interspecific hybridizations and population structure in the endangered cichlid Oreochromis mossambicus

2013

Hybridization between invasive and native species accounts among the major and pernicious threats to biodiversity. The Mozambique tilapia Oreochromis mossambicus, a widely used freshwater aquaculture species, is especially imperiled by this phenomenon since it is recognized by the IUCN as an endangered taxon due to genetic admixture with O. niloticus an invasive congeneric species. The Lower Limpopo and the intermittent Changane River (Mozambique) drain large wetlands of potentially great importance for conservation of O. mossambicus, but their populations have remained unstudied until today. Therefore we aimed (1) to estimate the autochthonous diversity and population structure among genet…

Fast Isolation by AFLP of Sequences Containing Repeats

2013

Fast isolation by AFLP of sequences containing repeats (FIASCO) is a rapid and simple method for separating microsatellite-containing DNA fragments from genomic DNA de novo. The method takes the advantage of the amplified fragment length polymorphism (AFLP) technique that relies on effective digestion-ligation reaction. The repeat-containing fragments are selectively hybridized to biotinylated probes and harvested by streptavidin-coated magnetic beads. The enriched microsatellite-containing fragments can be cloned and sequenced to yield a variety of microsatellite loci for applications in many different fields in molecular genetics.

Nephrotoxic drug burden among 1001 critically ill patients: impact on acute kidney injury.

2019

Abstract Background Nephrotoxic drug prescription may contribute to acute kidney injury (AKI) occurrence and worsening among critically ill patients and thus to associated morbidity and mortality. The objectives of this study were to describe nephrotoxic drug prescription in a large intensive-care unit cohort and, through a case–control study nested in the prospective cohort, to evaluate the link of nephrotoxic prescription burden with AKI. Results Six hundred and seventeen patients (62%) received at least one nephrotoxic drug, among which 303 (30%) received two or more. AKI was observed in 609 patients (61%). A total of 351 patients were considered as cases developing or worsening AKI a gi…

Somatosensory amplification in hypochondriasis and panic disorder

1999

The aim of this study is to explore the differences and similarities of Somatic Amplification (SA) in both panic and hypochondriasis disorders. An additional objective is to validate the Somatosensory Amplification Scale (SSAS) for use in our cultural context. For these purposes, 34 patients (17 with hypochondriasis and 17 with panic disorder; DSM-III-R criteria) completed the following questionnaires: SSAS, State–Trait Anxiety Inventory, Beck Depression Inventory, MMPI Hypochondriasis Scale, Illness Attitude Scales and Illness Behaviour Questionnaire. The SSAS showed a satisfactory internal consistency (Cronbach's α=0·83), and revealed two components: ‘Internal Stimulus Amplification’ and …