Search results for "PORES"
showing 10 items of 155 documents
Response ofBacillus subtilis spores to dehydration and UV irradiation at extremely low temperatures
1996
Spores of Bacillus subtilis have been exposed to the conditions of extreme dehydration (argon/silica gel; simulated space vacuum) for up to 12 weeks at 298 K and 80 K in the dark. The inactivation has been correlated with the production of DNA-double strand-breaks. The temperature-dependence of the rate constants for inactivation or production of DNA-double strand-breaks is surprisingly low. Controls kept in the frozen state at 250 K for the same period of time showed no sign of deterioration. In another series of experiments the spores have been UV irradiated (253.7 nm) at 298 K, 200 K and 80 K after exposure to dehydrating conditions for 3 days. Fluence-effect relationships for inactivati…
Identification of the mstE Gene Encoding a Glucose-inducible, Low Affinity Glucose Transporter in Aspergillus nidulans
2006
The mstE gene encoding a low affinity glucose transporter active during the germination of Aspergillus nidulans conidia on glucose medium has been identified. mstE expression also occurs in hyphae, is induced in the presence of other repressing carbon sources besides glucose, and is dependent on the function of the transcriptional repressor CreA. The expression of MstE and its subcellular distribution have been studied using a MstE-sGFP fusion protein. Concordant with data on mstE expression, MstE-sGFP is synthesized in the presence of repressing carbon sources, and fluorescence at the periphery of conidia and hyphae is consistent with MstE location in the plasma membrane. Deletion of mstE …
Development of multidimensional spectroscopic and microscopic techniques to facilitate the monitoring of native fluorescence of biomolecules.
2022
There is a need for the development of rapid and reliable characterization tools for biological media. The objective of this thesis is to develop a method based on the acquisition of excitation-emission matrices of fluorescence (EEMF) coupled with the use of fluorescence lifetime measurement in spectroscopy and microscopy (FLIM). These techniques have great potential due to their speed, low sample volume required for analysis, non-destructive sample analysis, and low cost. This project focused on two biological media of great interest to the food industry: wine and bacterial spores. On one hand, we have a beverage representing a large world market, and on the other hand, a food contaminant …
Combined Effects of 50 Hz Electromagnetic Field and SiO2 Nanoparticles on Oxidative Stress in Plant’s Gametic Cells / Zemfrekvences (50 Hz) Elekromag…
2015
Abstract The paper presents the results of combined effects of an extremely low frequency electromagnetic field (50 Hz) (ELF EMF) and SiO2 nanoparticles on fluorescence of plant gametic cells (immature microspores). The data were recorded by a BD FACSJazz® cell sorter after cell irradiation by blue laser (488 nm). A significant difference of fluorescence was observed between gametic cells after 1 hour incubation in suspension of SiO2 nanoparticles and the control gametic cells. It was observed that fluorescence intensity of gametic cell was higher with ELF EMF treatment in comparison to control cells, but it was statistically significant only for cells treated with electromagnetic radiation…
3D-Ultrastructure, Functions and Stress Responses of Gastropod (Biomphalaria glabrata) Rhogocytes
2014
Rhogocytes are pore cells scattered among the connective tissue of different body parts of gastropods and other molluscs, with great variation in their number, shape and size. They are enveloped by a lamina of extracellular matrix. Their most characteristic feature is the "slit apparatus", local invaginations of the plasma membrane bridged by cytoplasmic bars, forming slits of ca. 20 nm width. A slit diaphragm creates a molecular sieve with permeation holes of 20×20 nm. In blue-blooded gastropods, rhogocytes synthesize and secrete the respiratory protein hemocyanin, and it has been proposed-though not proven-that in the rare red-blooded snail species they might synthesize and secrete the he…
Effects of high pressure processing and biopreservation on the inactivation and the germination of spores of Bacillus and Clostridium
2018
Bacterial endospores are one of the most resistant life form on earth. Their capacity to survive to decontamination processes and their potential pathogenicity represent a real problem for the food industry. Currently, the only way to prevent their development in foods is the application of thermal treatments or the use of preservatives. However, these two methods have negative impacts on the nutritional properties of foods and on the consumers’ health. High hydrostatic pressure (HP) is a non-thermal process widely used for commercial pasteurization of foods. However, this process has a very low effect on spores and has therefore to be combined with other decontamination processes to enhanc…
Conical nanopores highlight the pro-aggregating effects of pyrimethanil fungicide on Aβ(1-42) peptides and dimeric splitting phenomena.
2022
International audience; The Aβ(1-42) aggregation is a key event in the physiopathology of Alzheimer's disease (AD). Exogenous factors such as environmental pollutants, and more particularly pesticides, can corrupt Aβ(1-42) assembly and could influence the occurrence and pathophysiology of AD. However, pesticide involvement in the early stages of Aβ(1-42) aggregation is still unknown. Here, we employed conical track-etched nanopore in order to analyse the Aβ(1-42) fibril formation in the presence of pyrimethanil, a widely used fungicide belonging to the anilinopyrimidine class. Our results evidenced a pro-aggregating effect of pyrimethanil on Aβ(1-42). Aβ(1-42) assemblies were successfully d…
Growth of two-dimensional Au patches in graphene pores: A density-functional study
2017
Inspired by recent studies of various two-dimensional (2D) metals such as Au, Fe and Ag, we study the growth of two-dimensional gold patches in graphene pores by density-functional theory. We find that at room temperature gold atoms diffuse readily on top of both graphene and two-dimensional gold with energy barriers less than $0.5$ eV. Furthermore, gold atoms move without barriers from the top of graphene to its edge and from the top of 2D gold to its edge. The energy barriers are absent even at the interface of 2D gold and graphene, so that the gold atoms move effortlessly across the interface. We hope our demonstration for the propensity of diffusing gold atoms to grow 2D gold patches in…
Highly selective chemical sensing in a luminescent nanoporous magnet.
2012
Among the wide variety of properties of interest that a given material can exhibit, luminescence is attracting an increasing attention due to its potential application in optical devices for lighting equipment and optical storage, [ 1a − c] optical switching, [ 1d ,e] and sensing. [ 1f − i ] At this respect, many scientists, working in the multidisciplinary fi eld of the materials science, have directed their efforts to the obtention of luminescent materials with potential sensing applications. For instance, sensitive and selective detection of gas and vapor phase analytes can result specially interesting because of the variety of applications that can be found in many different fi elds. A …
Effects of high-pressure processing on fungi spores: Factors affecting spore germination and inactivation and impact on ultrastructure.
2020
Food contamination with heat-resistant fungi (HRF), and their spores, is a major issue among fruit processors, being frequently found in fruit juices and concentrates, among other products, leading to considerable economic losses and food safety issues. Several strategies were developed to minimize the contamination with HRF, with improvements from harvesting to the final product, including sanitizers and new processing techniques. Considering consumers' demands for minimally processed, fresh-like food products, nonthermal food-processing technologies, such as high-pressure processing (HPP), among others, are emerging as alternatives to the conventional thermal processing techniques. As no …