Search results for "Pill"

showing 10 items of 1870 documents

BRAFV600E mutation, TIMP-1 upregulation, and NF-κB activation: closing the loop on the papillary thyroid cancer trilogy.

2011

BRAFV600E is the most common mutation found in papillary thyroid carcinoma (PTC). Tissue inhibitor of metalloproteinases (TIMP-1) and nuclear factor (NF)-κB have been shown to play an important role in thyroid cancer. In particular, TIMP-1 binds its receptor CD63 on cell surface membrane and activates Akt signaling pathway, which is eventually responsible for its anti-apoptotic activity. The aim of our study was to evaluate whether interplay among these three factors exists and exerts a functional role in PTCs. To this purpose, 56 PTC specimens were analyzed for BRAFV600E mutation, TIMP-1 expression, and NF-κB activation. We found that BRAFV600E mutation occurs selectively in PTC nodules an…

MAPK/ERK pathwayAdultMaleProto-Oncogene Proteins B-rafCancer Researchmedicine.medical_specialtyendocrine system diseasesEndocrinology Diabetes and MetabolismThyroid cancer TIMP-1 papillary thyroid cancerMutation MissenseGlutamic AcidGene Expression Regulation EnzymologicSettore MED/13 - EndocrinologiaPapillary thyroid cancerEndocrinologyDownregulation and upregulationInternal medicinemedicineTumor Cells CulturedGene silencingHumansGene Regulatory NetworksNeoplasm InvasivenessThyroid NeoplasmsProtein kinase BThyroid cancerTissue Inhibitor of Metalloproteinase-1ChemistryAkt/PKB signaling pathwayCarcinomaNF-kappa BValineMiddle Agedmedicine.diseaseCarcinoma PapillaryUp-RegulationGene Expression Regulation NeoplasticEndocrinologyCell Transformation NeoplasticOncologyAmino Acid SubstitutionThyroid Cancer PapillaryCancer researchDisease ProgressionFemaleV600ESignal TransductionEndocrine-related cancer
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Polymeric monolithic microcartridges with gold nanoparticles for the analysis of protein biomarkers by on-line solid-phase extraction capillary elect…

2020

In this study, polymeric monoliths with gold nanoparticles (AuNP@monolith) were investigated as microcartridges for the analysis of protein biomarkers by on-line solid-phase extraction capillary electrophoresis-mass spectrometry (SPE-CE-MS). “Plug-and-play” microcartridges (7 mm) were prepared from a glycidyl methacrylate (GMA)-based monolithic capillary column (5 cm x 250 µm i.d.), which was modified with ammonia and subsequently functionalized with gold nanoparticles (AuNPs). The performance of these novel microcartridges was evaluated with human transthyretin (TTR), which is a protein related to different types of familial amyloidotic polyneuropathies (FAP). Protein retention depended on…

MASS SPECTROMETRYPolymersMetal NanoparticlesOr010402 general chemistryMass spectrometry01 natural sciencesBiochemistryCapillary electrophoresis–mass spectrometryMass SpectrometryAnalytical Chemistry//purl.org/becyt/ford/1 [https]Capillary electrophoresisCAPILLARY ELECTROPHORESISLimit of DetectionElectroforesi capil·lar//purl.org/becyt/ford/1.4 [https]HumansPrealbuminSolid phase extractionChromatographyNanopartículesChemistryElutionSolid Phase Extraction010401 analytical chemistryOrganic ChemistryON-LINE SOLID-PHASE EXTRACTIONElectrophoresis CapillaryReproducibility of ResultsGeneral MedicineMONOLITHIC MICROCARTRIDGETRANSTHYRETIN0104 chemical sciencesEspectrometria de massesIsoelectric pointColloidal goldGOLD NANOPARTICLESEpoxy CompoundsMethacrylatesNanoparticlesGoldIon trapBiomarkers
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Sodium butyrate with UCN-01 has marked antitumour activity against cervical cancer cells.

2010

The effect of combining sodium butyrate (NaB), a histone deacetylase inhibitor, and 7-hydroxy-staurosporine (UCN-01) on cytotoxicity in human cervical carcinoma cells was evaluated.HeLa and CaSki cells were treated using NaB alone or in combination with staurosporine (STS) or its analog UCN-01. Cytotoxicity was determined by flow cytometry and morphological assays. Apoptotic pathways were characterized by Western blotting and immunostaining. CaSki cells were also xenografted into nude mice to assess the in vivo effects of NaB/UCN-01 combination.Treatment with NaB and STS or UCN-01 resulted in enhanced apoptosis of cancer cells. Apoptosis involved mitochondrial pathways and overexpression of…

MESH : StaurosporineMESH : Hela CellsMESH : Antineoplastic Combined Chemotherapy Protocolshealth care facilities manpower and servicesUterine Cervical NeoplasmsMESH: ButyratesMESH: Cell CycleApoptosisMESH: Papillomavirus Infections[ SDV.CAN ] Life Sciences [q-bio]/CancerMiceAntineoplastic Combined Chemotherapy ProtocolsMESH: AnimalsMESH: Human papillomavirus 18MESH : Human papillomavirus 18MESH : Femalehealth care economics and organizationsMESH: Human papillomavirus 16MESH : Papillomavirus InfectionsHuman papillomavirus 16Human papillomavirus 18Cell CycleMESH : Mice NudeMESH: Uterine Cervical NeoplasmsMESH: Antineoplastic Combined Chemotherapy ProtocolsButyratesMESH: Cell Growth ProcessesFemaleMESH: Xenograft Model Antitumor Assaysendocrine systemMESH: Cell Line TumoreducationMESH : Uterine Cervical NeoplasmsMice Nude[SDV.CAN]Life Sciences [q-bio]/CancerCell Growth ProcessesMESH : Xenograft Model Antitumor Assays[SDV.CAN] Life Sciences [q-bio]/CancerCell Line TumorMESH : ButyratesMESH : MiceMESH : Cell CycleMESH: Mice Nudeotorhinolaryngologic diseasesAnimalsHumansMESH: MiceMESH: HumansMESH : Cell Line TumorMESH: ApoptosisPapillomavirus InfectionsMESH : HumansMESH : Human papillomavirus 16StaurosporineXenograft Model Antitumor AssaysMESH: Hela CellsMESH : Cell Growth ProcessesMESH: StaurosporineMESH : AnimalsMESH: FemaleMESH : ApoptosisHeLa Cells
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[Vaccination against papillomavirus : arguments and evidence of effectiveness].

2020

Vaccination against papillomavirus: arguments and evidence of effectiveness. Vaccination against human papillomavirus is a major advance in the prevention of cervical cancer. Evidence of its effectiveness has accumulated over the past thirty years since basic research has demonstrated the ability of viral pseudoparticles to induce immune responses in animals. Large human clinical trials followed to demonstrate the safety and efficacy of vaccination against targeted HPV infections and their associated lesions. After its approval and marketing the vaccine efficacy was measured at the level of entire populations, confirming its effectiveness and medical interest. Today, models predict a possib…

MESH: HumansPapillomavirus InfectionsVaccinationUterine Cervical Neoplasms[SDV.CAN]Life Sciences [q-bio]/CancerMESH: Papillomavirus VaccinesMESH: VaccinationMESH: Papillomavirus InfectionsMESH: Uterine Cervical Neoplasms[SDV.CAN] Life Sciences [q-bio]/CancerHumansFemalePapillomavirus VaccinesDisease EradicationMESH: FemaleLa Revue du praticien
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Technology spillover effects within Spanish communities

2017

ABSTRACTThe article uses panel data for the period 1990–2010 to estimate technology spillover effects on 17 Spanish communities. Accounting for nonstationarity and cointegration, we use the dynamic OLS estimator to estimate the impact of domestic and non-domestic R&D capital stock on labour productivity of Spanish communities, taking into account trade-, migration- and foreign direct investment (FDI)-related technology diffusion channels. We find significant trade-related spillover effects within Spanish communities and from EU countries. On average, an increase in the non-domestic R&D stock of 1% increases their labour productivity between 0.02% and 0.12% if related to bilateral trade patt…

MacroeconomicsEconomics and EconometricsCointegration05 social sciencesForeign direct investmentOecd countriesCapital stockBilateral tradeSpillover effect0502 economics and businessEconomicsDemographic economics050207 economicsStock (geology)050205 econometrics Panel dataApplied Economics
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Technology spillover and TFP growth: A spatial Durbin model

2016

Beginning with a model in which technological progress is reflected by product variety, we provide a structural approach to estimate technology spillovers allowing for spatial interdependencies. To this end, we first present a theoretical model of TFP growth by decomposing TFP into quality and variety components. We address the quality component by introducing a country׳s distance to the technological frontier. Quality is assumed to be a negative function of the technological gap of country i with respect to its own technological frontier. This technological threshold is defined as the geometric means of knowledge levels in all countries. We deal with the variety component by using R&D expe…

MacroeconomicsTFP GrowthTechnological changemedia_common.quotation_subject05 social sciences[SHS.ECO]Humanities and Social Sciences/Economics and FinanceSpatial Durbin ModelGeneral Business Management and AccountingHuman capitalInterdependenceSpillover effectTechnology Spillover0502 economics and businessEconomicsEconometrics[ SHS.ECO ] Humanities and Social Sciences/Economies and finances050207 economics[SHS.ECO] Humanities and Social Sciences/Economics and FinanceGeneral Economics Econometrics and FinanceTotal factor productivityProductivitySpatial analysisStock (geology)050205 econometrics media_commonInternational Economics
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Suppression of capillary wave broadening of interfaces in binary alloys due to elastic interactions.

2005

By Monte Carlo simulations in the constant-temperature--constant-pressure ensemble a planar interface between unmixed A-rich and B-rich phases of a binary (A, B) alloy on a compressible diamond lattice is studied. No significant capillary wave broadening of the concentration profile across the interface is observed, unlike lattice models of incompressible mixtures and fluids. The distortion of the lattice structure across the interface is studied.

MagnetizationCapillary wavePlanarMaterials scienceCondensed matter physicsLattice (order)Monte Carlo methodCompressibilityGeneral Physics and AstronomyCrystal structureDiamond cubicPhysical review letters
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Immunomorphological Pattern of Molecular Chaperones in Normal and Pathological Thyroid Tissues and Circulating Exosomes: Potential Use in Clinics

2019

The thyroid is a major component of the endocrine system and its pathology can cause serious diseases, e.g., papillary carcinoma (PC). However, the carcinogenic mechanisms are poorly understood and clinical useful biomarkers are scarce. Therefore, we determined if there are quantitative patterns of molecular chaperones in the tumor tissue and circulating exosomes that may be useful in diagnosis and provide clues on their participation in carcinogenesis. Hsp27, Hsp60, Hsp70, and Hsp90 were quantified by immunohistochemistry in PC, benign goiter (BG), and normal peritumoral tissue (PT). The same chaperones were assessed in plasma exosomes from PC and BG patients before and after ablative surg…

Male0301 basic medicineGoiterdiagnosismedicine.disease_causelcsh:Chemistry0302 clinical medicinelcsh:QH301-705.5Heat-Shock ProteinsSpectroscopygoiterbiologyThyroidmolecular chaperonesGeneral MedicineMiddle Agedmolecular chaperone3. Good healthComputer Science ApplicationsBlotdiagnosimedicine.anatomical_structure030220 oncology & carcinogenesisheat shock proteins (Hsp)ImmunohistochemistryFemalethyroid gland; papillary carcinoma; molecular chaperones; heat shock proteins (Hsp); goiter; exosomes; diagnosiendocrine systemanimal structuresexosomesArticleCatalysisInorganic Chemistry03 medical and health sciencesHsp27medicineHumansEndocrine systemexosomePhysical and Theoretical ChemistryMolecular Biologythyroid glandbusiness.industryOrganic Chemistrymedicine.diseaseCarcinoma PapillaryMicrovesicles030104 developmental biologylcsh:Biology (General)lcsh:QD1-999Cancer researchbiology.proteinCarcinogenesisbusinesspapillary carcinomaInternational Journal of Molecular Sciences
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The LepR-mediated leptin transport across brain barriers controls food reward

2018

Objective Leptin is a key hormone in the control of appetite and body weight. Predominantly produced by white adipose tissue, it acts on the brain to inhibit homeostatic feeding and food reward. Leptin has free access to circumventricular organs, such as the median eminence, but entry into other brain centers is restricted by the blood–brain and blood–CSF barriers. So far, it is unknown for which of its central effects leptin has to penetrate brain barriers. In addition, the mechanisms mediating the transport across barriers are unclear although high expression in brain barriers suggests an important role of the leptin receptor (LepR). Methods We selectively deleted LepR in brain endothelia…

Male0301 basic medicineLeptinHFD high-fat dietEndothelial cellsWhite adipose tissueCSF cerebrospinal fluidMice0302 clinical medicineCPP conditioned place preferenceBBB blood–brain barrierCells Culturedmedia_commonLeptindigestive oral and skin physiologyi.p. intraperitonealmedicine.anatomical_structureLepRBlood-Brain BarrierBlood–brain barrier; Endothelial cells; LepR; Leptin; Obesity; RewardMedian eminenceqPCR quantitative polymerase chain reactionReceptors LeptinOriginal ArticleChoroid plexusmedicine.medical_specialtylcsh:Internal medicinemedia_common.quotation_subjectHyperphagiaBiologyBlood–brain barrierVTA ventral tegmental areaBC bottle choice testCapillary PermeabilityBlood–brain barrierARC arcuate nucleus03 medical and health sciencesPBS phosphate buffered salineRewardInternal medicinemedicineAnimalsObesitylcsh:RC31-1245Molecular BiologyCircumventricular organsBlood-Nerve BarrierLeptin receptorNCD normal chow dietAppetiteCell Biology030104 developmental biologyEndocrinologyLepR leptin receptorChoroid PlexusBSA bovine serum albuminPFA paraformaldehyde030217 neurology & neurosurgeryDAPI 4′6-diamidino-2-phenylindoleMolecular Metabolism
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Primordial odontogenic tumor: An immunohistochemical profile.

2017

Background Primordial Odontogenic Tumor (POT) is a recently described odontogenic tumor characterized by a variably cellular loose fibrous tissue with areas similar to the dental papilla, covered by cuboidal to columnar epithelium that resembles the internal epithelium of the enamel organ, surrounded at least partly by a delicate fibrous capsule. The purpose of this study was to investigate the possible histogenesis and biological behavior of this rare tumor by means of a wide immunohistochemical analysis of its epithelial and mesenchymal components. Material and Methods The immunoexpression of twenty-three different antibodies were evaluated in four cases of POT. Results The epithelial cel…

Male0301 basic medicinePathologymedicine.medical_specialtyAdolescentAntibodies NeoplasmOdontogenic TumorsVimentinHistogenesisRADIOGRAFIA03 medical and health sciences0302 clinical medicineprimordial.medicineHumansPTENDental papillaGeneral DentistryJaw tumorsOral Medicine and PathologybiologyTUMORES ODONTOGENICOSNEOPLASIAS MAXILOMANDIBULARESResearchMesenchymal stem cellodontogenicEnamel organOdontogenic tumorEndoglin:CIENCIAS MÉDICAS [UNESCO]medicine.diseaseImmunohistochemistryJaw Neoplasms030104 developmental biologyOtorhinolaryngologyChild Preschool030220 oncology & carcinogenesisUNESCO::CIENCIAS MÉDICASbiology.proteinFemaleSurgery
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