Search results for "Point spread function"
showing 10 items of 75 documents
Quasi-spherical focal spot in two-photon scanning microscopy by three-ring apodization
2005
International audience; We present a beam-shaping technique for two-photon excitation (TPE) fluorescence microscopy. We show that by inserting a properly designed three-ring pupil filter in the illumination beam of the microscope, the effective optical sectioning capacity of such a system improves so that the point spread function gets a quasi-spherical shape. Such an improvement, which allows the acquisition of 3D images with isotropic quality, is obtained at the expense of only a small increase of the overall energy in the axial sidelobes. The performance of this technique is illustrated with a scanning TPE microscopy experiment in which the image of small beads is obtained. We demonstrat…
Total Variation Based Image Restoration
2004
For the purpose of image restoration the process of image formation can be modeled in a first approximation by the formula [207] $$ {u_d} = Q\{ II(k*u) + n\} , $$ (1.1) where u represents the photonic flux k is the point spread function of the optical-captor joint apparatus П is a sampling operator, i.e., a Dirac comb supported by the centers of the matrix of digital sensors, n represents a random perturbation due to photonic or electronic noise, and Qis a uniform quantization operator mapping ℝ to a discrete interval of values, typically [0, 255].
Resolution enhancement in integral microscopy by physical interpolation
2015
Integral-imaging technology has demonstrated its capability for computing depth images from the microimages recorded after a single shot. This capability has been shown in macroscopic imaging and also in microscopy. Despite the possibility of refocusing different planes from one snap-shot is crucial for the study of some biological processes, the main drawback in integral imaging is the substantial reduction of the spatial resolution. In this contribution we report a technique, which permits to increase the two-dimensional spatial resolution of the computed depth images in integral microscopy by a factor of √2. This is made by a double-shot approach, carried out by means of a rotating glass…
Clinical use of the ocular point spread function for retinal image quality assessment
2008
The purpose of this study is to demonstrate the clinical use of the point spread function (PSF) as metric for the assessment of retinal image quality in eyes with different ocular conditions. The PSF was computed from the wavefront aberrations obtained in seven eyes with different ocular conditions: emmetropia, myopia, hyperopia, post-myopic laser in situ keratomileusis (LASIK), post-hyperopic LASIK, keratoconus, Intacs ® in keratoconus, radial keratotomy (RK), LASIK after RK, cataract, phacoemulsification after cataract surgery, and LASIK after astigmatic keratotomy. The Strehl intensity ratio was calculated for all the PSFs computed. The PSF was correlated with the change in the optical e…
Multiframe image restoration in the presence of noisy blur kernel
2009
We wish to recover an original image u from several blurry-noisy versions f k , called frames. We assume a more severe degradation model, in which the image u has been blurred by a noisy (stochastic) point spread function. We consider the problem of restoring the degraded image in a variational framework. Since the recovery of u from one single frame f is a highly ill-posed problem, we formulate two minimization problems based on the multiframe approach proposed for image super-resolution by Marquina-Osher [13]. Several experimental results for image restoration are shown, illustrating that the proposed models give visually satisfactory results.
Through-focus response of multifocal intraocular lenses evaluated with a spatial light modulator
2012
A new testing technique based on the use of a liquid crystal spatial light modulator (SLM) is proposed to analyze the optical quality of multifocal intraocular lenses (MIOLs). Different vergences and decentrations of the incident beam can be programmed onto the SLM in order to record the point spread function (PSF) for different object positions. From these axial PSFs, the through-focus modulation transfer function is computed. Because there are no moving parts in the experimental setup, this method is fast and versatile to assess MIOLs. Experimental results confirm the potential of the proposed method.
Fast multi-directional DSLM for confocal detection without striping artifacts
2020
In recent years light-sheet fluorescence microscopy (LSFM) has become a cornerstone technology for neuroscience, improving the quality and capabilities of 3D imaging. By selectively illuminating a single plane, it provides intrinsic optical sectioning and fast image recording, while minimizing out of focus fluorescence background, sample photo-damage and photo-bleaching. However, images acquired with LSFM are often affected by light absorption or scattering effects, leading to un-even illumination and striping artifacts. In this work we present an optical solution to this problem, via fast multi-directional illumination of the sample, based on an acousto-optical deflector (AOD). We demonstr…
Tunable axial superresolution by annular binary filters. Application to confocal microscopy
1995
We present a set of annular binary pupil filters for increasing the axial resolving capacity of imaging systems. The filters consist of two transparent annuli of the same area. It is shown that by changing the area of the transparent regions it is possible to obtain a tunable reduction of the width of the central lobe of the axial point spread function of the imaging system. However, this reduction is accompanied by a severe increase of the strength of secondary lobes, what can make these filters not very useful when used in conventional imaging systems. That is why we propose to use these filters for apodizing confocal microscopy systems. It is shown that in this case an important reductio…
Axial resolution in two-color excitation fluorescence microscopy by phase-only binary apodization
2005
We study the effect of a kind of binary phase-only filters, the Toraldo filters, in two-color excitation fluorescence microscopy. We show that by simple insertion of a properly designed Toraldo filter in one of the illumination arms the axial resolution of the system is significantly improved. Specifically, the main peak of the point spread function is narrowed by 22% along the axial direction.
Long working range light field microscope with fast scanning multifocal liquid crystal microlens array
2018
The light field microscope has the potential of recording the 3D information of biological specimens in real time with a conventional light source. To further extend the depth of field to broaden its applications, in this paper, we proposed a multifocal high-resistance liquid crystal microlens array instead of the fixed microlens array. The developed multifocal liquid crystal microlens array can provide high quality point spread function in multiple focal lengths. By adjusting the focal length of the liquid crystal microlens array sequentially, the total working range of the light field microscope can be much extended. Furthermore, in our proposed system, the intermediate image was placed i…