Search results for "QP"
showing 10 items of 610 documents
Influence of Aerobic Exercise After Static Stretching on Flexibility and Strength in Plantar Flexor Muscles
2020
Aerobic exercise could improve stretch-induced strength deficits. However, mechanisms of the improvement were unclear. The purpose of the study was to examine the effects of aerobic exercise after static stretching (SS) on flexibility and isometric strength in ankle plantar-flexor muscles. Fifteen healthy males received two interventions after SS of their ankle plantar-flexor muscles for 5 min. One was aerobic exercise for 10-min on a cycling ergometer, and the other was a 10-min rest as a control. Range of motion (ROM) of ankle dorsiflexion, passive torque at terminal ROM, muscle-tendon unit (MTU) stiffness, muscle tendon junction displacement, peak torque of ankle plantarflexion, and the …
Effect of Training-Induced Changes in Achilles Tendon Stiffness on Muscle–Tendon Behavior During Landing
2018
During rapid deceleration of the body, tendons buffer part of the elongation of the muscle–tendon unit (MTU), enabling safe energy dissipation via eccentric muscle contraction. Yet, the influence of changes in tendon stiffness within the physiological range upon these lengthening contractions is unknown. This study aimed to examine the effect of training-induced stiffening of the Achilles tendon on triceps surae muscle–tendon behavior during a landing task. Twenty-one male subjects were assigned to either a 10-week resistance-training program consisting of single-leg isometric plantarflexion (n = 11) or to a non-training control group (n = 10). Before and after the training period, plantarf…
Longer Nap Duration During Ramadan Observance Positively Impacts 5-m Shuttle Run Test Performance Performed in the Afternoon.
2022
It is well-documented that changes in the rhythm of life during Ramadan affect sleep schedules (i.e., interruption of night sleep patterns) and are likely to have negative effects on physical and cognitive performances. The aim of the present study was to examine the effect of different naps opportunities’ durations during Ramadan on performance of short-duration repetitive maximal exercise and perception of effort. Fifteen physically active men (age: 21 ± 3 years, height: 177 ± 6 cm, body-mass: 73 ± 10 kg) performed a 6 × 30-s shuttle run test after a 25-min nap (N25), a 45-min nap (N45), and in a no-nap condition (NN) during three experimental periods: ∼2 weeks before Ramadan (BR), the la…
The corticotrophin-releasing factor/urocortin system regulates white fat browning in mice through paracrine mechanisms.
2015
Objectives:\ud The corticotrophin-releasing factor (CRF)/urocortin system is expressed in the adipose tissue of mammals, but its functional role in this tissue remains unknown.\ud \ud Methods:\ud Pharmacological manipulation of the activity of CRF receptors, CRF1 and CRF2, was performed in 3T3L1 white pre-adipocytes and T37i brown pre-adipocytes during in vitro differentiation. The expression of genes of the CRF/urocortin system and of markers of white and brown adipocytes was evaluated along with mitochondrial biogenesis and cellular oxygen consumption. Metabolic evaluation of corticosterone-deficient or supplemented Crhr1-null (Crhr1−/−) mice and their wild-type controls was performed alo…
Cold Exposure Affects Lipid Metabolism, Fatty Acids Composition and Transcription in Pig Skeletal Muscle
2021
Cold exposure promotes glucose oxidation and modulates the lipid metabolism in adipose tissue, but it is still not fully clear whether cold exposure could affect meat quality and fatty acid metabolism in skeletal muscle of pig in vivo. Here, we kept finishing pigs under cold or room temperature overnight and determined the effects of cold exposure on meat quality, fatty acids composition and transcriptional changes in skeletal muscle of pigs. We found that cold exposure significantly reduced the meat colour24 h and pH24 h, without affecting carcass characteristics and other meat quality traits. Considerable changes were found in the proportions of individual fatty acids and the total conten…
.Single-Ion Magnetic Behaviour in an Iron(III) Porphyrin Complex: A Dichotomy Between High Spin and 5/2-3/2 Spin Admixture
2020
International audience; A mononuclear iron(III) porphyrin compound exhibiting unexpectedly slow magnetic relaxation, which is a characteristic of single-ion magnet behaviour, is reported. This behaviour originates from the close proximity (approximate to 550 cm(-1)) of the intermediate-spinS=3/2 excited states to the high-spinS=5/2 ground state. More quantitatively, although the ground state is mostlyS=5/2, a spin-admixture model evidences a sizable contribution (approximate to 15 %) ofS=3/2 to the ground state, which as a consequence experiences large and positive axial anisotropy (D=+19.2 cm(-1)). Frequency-domain EPR spectroscopy allowed them(S)= |+/- 1/2⟩->|+/- 3/2&Rig…
Data from: Quantitative PCR primer design affects quantification of dsRNA-mediated gene knockdown
2020
RNA interference (RNAi) is a powerful tool for studying functions of candidate genes in both model and non-model organisms and a promising technique for therapeutic applications. Successful application of this technique relies on the accuracy and reliability of methods used to quantify gene knockdown. With the limitation in the availability of antibodies for detecting proteins, quantitative PCR (qPCR) remains the preferred method for quantifying target gene knockdown after dsRNA treatment . We evaluated how qPCR primer binding site and target gene expression levels affect quantification of intact mRNA transcripts following dsRNA-mediated RNAi. The use of primer pairs targeting the mRNA sequ…
SAFER : le temps de la rétrocession respectueux de la Constitution
2018
International audience; L’absence de sanction de l’obligation pour la SAFER de rétrocéder un bien préempté dans le délai de 5 ans ne porte pas une atteinte disproportionnée au droit de propriété, à la liberté contractuelle et à la liberté d'entreprendre protégés par les articles 2 et 4 de la Déclaration des droits de l’homme et du citoyen. Cons. Const. DC n° 2018-707, QPC, 25 mai 2018
Subtelomēru rajona hromatīna struktūras izmaiņu noteikšana ar qPCR
2015
Cilvēka subtelomēri ir izteikti polimorfiski un ir ar vislielāko gēnu blīvumu visā genomā. Subtelomēru sekvences pārmaiņas ir saistītas ar mentālo atpalicību, ļaundabīgām hematoloģiskajām slimībām, atkārtotiem spontānajiem abortiem un citām slimībām. Tādēļ subtelomēru reģiona pārmaiņu konstatēšana ir svarīga klīniskās izpētes sastāvdaļa. Subtelomēru pētniecībā galvenokārt tiek pielietotas divas metodes: FISH un SAT. Šajā darbā tika aprakstīta qPCR metode subtelomēru analīzei, kas, teorētiski ir lētāka, ātrāka un precīzāka alternatīva FISH un SAT metodēm. Darbā apraksta qPCR metodes pielietošanu, Y hromosomas p pleca subtelomēru reģiona pārmaiņu pētīšanai, 34 DNS paraugiem, kas iegūti no nea…
Trichoderma asperellum, Bacillus subtilis un to konsorcija ietekme uz augsnes mikroorganismiem
2019
Pētījuma mērķis bija noskaidrot, vai Trichoderma asperellum, Bacillus subtilis un to konsorcijs ietekmē augsnes mikrobiotu. Pētījumā eksperimentālie augsnes paraugi tika apstrādāti ar B. subtilis, T.asperellum un B. cinerea mikroorganismiem un to konsorcijiem. Izmantojot mikrobioloģiskās metodes, tika noteikts baktēriju un sēņu daudzums dažādi apstrādātās augsnēs. Ar Biolog EcoPlates metodi, noteikta mikroorganismu metaboliskā aktivitāte augsnē. Trichoderma ģints, Firmicutes, Gammaproteobacteria un Acidobacteria DNS koncentrācija augsnes paraugos tika noteikta ar molekulārajām metodēm. Tika veikta korelācijas analīze starp mikrobioloģisko un molekulāro un tikai starp dažādiem molekulāro rez…