Search results for "ROT"
showing 10 items of 41723 documents
A self-inducible heterologous protein expression system in Escherichia coli
2016
AbstractEscherichia coli is an important experimental, medical and industrial cell factory for recombinant protein production. The inducible lac promoter is one of the most commonly used promoters for heterologous protein expression in E. coli. Isopropyl-β-D-thiogalactoside (IPTG) is currently the most efficient molecular inducer for regulating this promoter’s transcriptional activity. However, limitations have been observed in large-scale and microplate production, including toxicity, cost and culture monitoring. Here, we report the novel SILEX (Self-InducibLe Expression) system, which is a convenient, cost-effective alternative that does not require cell density monitoring or IPTG inducti…
Standardised spider (Arachnida, Araneae) inventory of Lammi, Finland
2020
In June 2019, an ecology field course of the University of Helsinki was held at Lammi Biological Station, Southern Finland. Within this course, the students familiarised themselves with field work and identification of spiders and explored the diversity of species in the area. Three sampling plots were chosen, one in grassland and two in boreal forest, to demonstrate the sampling techniques and, by applying a standardised protocol (COBRA), contribute to a global spider biodiversity project. The collected samples contained a total of 3445 spiders, of which 1956 (57%) were adult. Only adult spiders were accounted for in the inventory due to the impossibility of identification of juveniles. A …
Changes in freshwater sediment microbial populations during fermentation of crude glycerol
2020
This work was supported by the Latvian Council of Science , project NN-CARMA, project No. lzp-2018/1-0194.
Successive Losses of Central Immune Genes Characterize the Gadiformes' Alternate Immunity.
2016
Great genetic variability among teleost immunomes, with gene losses and expansions of central adaptive and innate components, has been discovered through genome sequencing over the last few years. Here, we demonstrate that the innate Myxovirus resistance gene (Mx) is lost from the ancestor of Gadiformes and the closely related Stylephorus chordatus, thus predating the loss of Major Histocompatibility Complex class II (MHCII) in Gadiformes. Although the functional implication of Mx loss is still unknown, we demonstrate that this loss is one of several ancient events appearing in successive order throughout the evolution of teleost immunity. In particular, we find that the loss of Toll-like r…
Conjugation is necessary for a bacterial plasmid to survive under protozoan predation
2016
Horizontal gene transfer by conjugative plasmids plays a critical role in the evolution of antibiotic resistance. Interactions between bacteria and other organisms can affect the persistence and spread of conjugative plasmids. Here we show that protozoan predation increased the persistence and spread of the antibiotic resistance plasmid RP4 in populations of the opportunist bacterial pathogenSerratia marcescens. A conjugation-defective mutant plasmid was unable to survive under predation, suggesting that conjugative transfer is required for plasmid persistence under the realistic condition of predation. These results indicate that multi-trophic interactions can affect the maintenance of con…
The aminophosphonate glyphosine enhances phycobiliprotein yields from selected cyanobacterial cultures
2017
Among added-value products obtained from cyanobacterial cultures are phycobiliproteins, photosynthetic pigments that have found an increasing number of applications as natural dyes for food, cosmetics, pharmaceuticals, and antioxidants. To obtain sustainable production, we aimed at maximizing phycobilin yield through the increase of either the final biomass or the specific content of these pigments by varying culture parameters, such as chemical composition and pH of the medium or quality and intensity of the light. Here, we report that the addition to the culture medium of millimolar or submillimolar concentrations of the aminophosphonate glyphosine [(N,N-bis(phosphonomethyl)glycine], form…
Selenium biofortification and grafting modulate plant performance and functional features of cherry tomato grown in a soilless system
2021
Abstract Selenium (Se) is an essential trace element for humans due to its importance in a number of enzymes. Vegetable grafting is a valuable tool to overcome biotic and/or abiotic issues and to increase vigour, yield traits and fruit quality. The present work aimed at testing both different Se concentrations (0.0, 1.0, 2.0 and 4.0 μmol Se L−1) supplied via fertigation and grafting on cherry tomato in soilless culture. Se at 2.0 μmol L−1 improved total fruit yield by 60.0 % and 31.4 % in ungrafted and grafted plants, respectively as compared to the control. Marketable yield was positively affected by Se-biofortification and grafting. Se at 2.0 μmol L−1 improved N use efficiency by 60.3 % a…
Neurosporaxanthin Overproduction by Fusarium fujikuroi and Evaluation of Its Antioxidant Properties
2020
17 Páginas.-- 5 Figuras
Colorado potato beetle chymotrypsin genes are differentially regulated in larval midgut in response to the plant defense inducer hexanoic acid or the…
2019
When Colorado potato beetle larvae ingested potato plants treated with the plant defense inducer compound hexanoic acid, midgut chymotrypsin enzyme activity increased, and the corresponding chymotrypsin genes were differentially expressed, evidence of the larval digestive proteolytic system's plasticity. We previously reported increased susceptibility to Cry3Aa toxin in larvae fed hexanoic acid treated plants. Here we show that the most expressed chymotrypsin gene in larvae fed hexanoic acid treated plants, CTR6, was dramatically downregulated in Cry3Aa intoxicated larvae. lde-miR-965-5p and lde-miR-9a-5p microRNAs, predicted to target CTR6, might be involved in regulating the response to h…
cDNA sequences of two arylphorin subunits of an insect biliprotein: phylogenetic differences and gene duplications during evolution of hexamerins-imp…
2016
Arylphorins represent a conserved class of hexameric ∼500 kDa insect hemolymph glycoproteins, rich in aromatic amino acids, which are produced in large quantities at the larval stage as reserves for metamorphosis and egg development. The recently isolated arylphorin from the moth Cerura vinula is unique in being complexed to a novel farnesylated bilin. Protein sequencing suggested the presence of two different ∼85 kDa subunits. Here, we report the complete coding sequences of two cDNAs encoding two arylphorins subunits with 67% identity and calculated physicochemical characteristics in agreement with the isolated holoprotein. Our phylogenetic analyses of the hexamerins revealed monophyletic…