Search results for "Rhazya"
showing 8 items of 8 documents
Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications
2001
An efficient system for the analysis of indole alkaloids by HPLC on a reversed-phase column using an ion pair technique is described. The optimised chromatographic conditions allowed the successful separation of 22 standard monoterpenoid indole alkaloids (including some isomers) and tryptamine. The described HPLC system was applied to the analysis of alkaloids in intergeneric somatic hybrid cell cultures of Rauvolfia serpentina x Rhazya stricta.
Induction of alkaloid diversity in hybrid plant cell cultures
1999
The treatment of Rauwolfia serpentina x Rhazya stricta somatic hybrid cell suspension culture with 100 μM of methyl jasmonate led to a general increase in indole alkaloid content and to qualitative changes in the alkaloid pattern. The content of Six alkaloids were investigated with respect to their content in both the cell biomass and nutrition medium. Intracellular 17-O-acetyl-norajmaline content on the 5th day after treatment had increased about 40-fold compared with the control culture. The respective concentrations of the other alkaloids increased by a factor of two to five. In total 26 indole alkaloids were identified in extracts of the methyl jasmonate-treated culture by TLC, UV, MS a…
3-Oxo-rhazinilam: A New Indole Alkaloid from Rauvolfia serpentina × Rhazya stricta Hybrid Plant Cell Cultures
2000
A new monoterpenoid indole alkaloid, 3-oxo-rhazinilam (1), was isolated from intergeneric somatic hybrid cell cultures of Rauvolfia serpentina and Rhazya stricta, and the structure was determined by detailed 1D and 2D NMR analysis. It was also proved that 3-oxo-rhazinilam (1) is a natural constituent of the hybrid cells.
In vivo monitoring of alkaloid metabolism in hybrid plant cell cultures by 2D cryo-NMR without labelling
2003
Non-invasive measurements of alkaloid metabolism in plant cell suspension cultures of a somatic hybrid from Rauvolfia serpentina Benth. ex Kurz and Rhazya stricta Decaisne were carried out. When cell samples were taken sequentially from a stock feeding experiment, measuring times for in vivo NMR of 40 min were sufficient for following conversions of alkaloids at the natural abundance of 13C. Degradation of ajmaline added to the cells at 1.6 mM concentration to raumacline could be monitored after 96 h on a standard 800 MHz NMR instrument (Avance 800). Feeding vinorine an intermediate of ajmaline biosynthesis at 1.8 mM showed with a 500 MHz CryoProbe that the alkaloid enters two metabolic rou…
Alkaloids Isolated from Somatic Hybrid Cell Cultures of the Species CombinationRauwolfia serpentinaxrhazya stricta
1995
Abstract The phytochemical analyses of a somatic hybrid cell suspension culture of the genus and species combination Rauwolfia serpentina x Rhazya stricta revealed a number of monoterpenoid indole alkaloids. The alkaloids were identified as tubotaiwine, an isomeric mixture of vallesiachotamine, stemmadenine and vomilenine and were all produced in small amounts < 2mg/litre cell suspension. The hybrid cells showed a greater diversity of alkaloid biosynthesis when compared with the parental cultures.
Isolation and characterization of intergeneric somatic hybrids in the Apocynaceae family
1991
Protoplasts were isolated from callus cultures of Rauwolfia serpentina Benth., Rhazya stricta Decaisne, and Catharanthus roseus (L.) G. Don, or from leaves of Vinca minor L. Protoplast isolation, culture, and fusion techniques as well as hybrid screening systems were developed for these species, and hybrids were obtained. Hybrid combinations were Rauwolfia + Vinca, Rauwolfia + Catharanthus, Rauwolfia + Rhazya, and Catharanthus + Vinca. For hybrid isolation, the physiological complementation method was utilized. Analyses of the material obtained included a cytogenetic study of the chromosomes, a study of multiple molecular forms of transferases and esterases, and the blot hybridization of re…
Isolation of Alkaloids from Cultured Hybrid Cells of Rauwolfia serpentina*Rhazya stricta.
1996
Two monoterpenoid indole alkaloids and four β-carbolines were isolated from a hydrid cell suspension culture generated from two Apocynaceous plants, Rauwolfia serpentina Benth. and Rhazya stricta Decaisne. This indicates that the function of alkoloid biosynthesis is retained after hybrid formation and that alkaloids not previously detected in the parental plants or cell cultures are formed.
Somatic Hybridization in the Family Apocynaceae (Catharanthus, Rauwolfia, Rhazya, and Vinca Species)
1994
The Apocynaceae family includes about 2000 species belonging to approximately 200 genera (Takhtadjan 1981). The majority of species originate in the tropics, only a few of them are common to Europe (Vinca minor, V. herhaceae, V. major, genus Trachomitum).