Search results for "SCOPE"

Common-path phase-shifting digital holographic microscopy: A way to quantitative phase imaging and superresolution

2008

We present an experimental setup useful for complex amplitude evaluation and phase image quantification of three-dimensional (3-D) samples in digital holographic microscopy (DHM). It is based on a common-path interferometric configuration performed by dividing the input plane in two contiguous regions and by placing a translation grating near to the Fourier plane. Then, complex amplitude distribution of the sample under test is recovered with phase-shifting standard method obtained by moving the grating using a linear motion stage. Some experimental results of an USAF resolution test are presented for different numerical aperture (NA) microscope lenses. In a second part, the proposed setup …

Four channels multi-illumination single-holographic-exposure lensless Fresnel (MISHELF) microscopy

2018

Abstract MISHELF microscopy [Opt. Express 23, 21352 (2015)] has been recently reported as the background technology of a new concept of compact, cost-effective and field-portable lensless microscope [Sci. Rep. 7, 43291 (2017)] based on wavelength multiplexing and a fast and robust algorithm for twin image minimization and noise reduction. In this manuscript, MISHELF microscopy is expanded beyond its actual configuration by considering 4 illumination/detection channels while retaining its working principle concerning single-shot, twin image mitigation and noise averaging. Proof of principle validation of the proposed improvement is conducted through experiments with a resolution test target …

2017

Micro-fluidic pumps as well as artificial micro-swimmers are conveniently realized exploiting phoretic solvent flows based on local gradients of temperature, electrolyte concentration or pH. We here present a facile micro-photometric method for monitoring pH gradients and demonstrate its performance and scope on different experimental situations including an electro-osmotic pump and modular micro-swimmers assembled from ion exchange resin beads and polystyrene colloids. In combination with the present microscope and DSLR camera our method offers a 2 \mu m spatial resolution at video frame rate over a field of view of 3920x2602 \mu m^2. Under optimal conditions we achieve a pH-resolution of …

SMIM in reflection imaging mode

2019

We present reflective SMIM (initials incoming from Spatially-Multiplexed Interferometric Microscopy) as an extremely simple and low cost way to convert a standard white-light microscope into a holographic one working under reflection imaging mode.

Hilbert-Huang single-shot spatially multiplexed interferometric microscopy.

2018

Hilbert-Huang single-shot spatially multiplexed interferometric microscopy (H2S2MIM) is presented as the implementation of a robust, fast, and accurate single-shot phase estimation algorithm with an extremely simple, low-cost, and highly stable way to convert a bright field microscope into a holographic one using partially coherent illumination. Altogether, H2S2MIM adds high-speed (video frame rate) quantitative phase imaging capability to a commercially available nonholographic microscope with improved phase reconstruction (coherence noise reduction). The technique has been validated using a 20×/0.46  NA objective in a regular Olympus BX-60 upright microscope for static, as well as dynamic…

Characterization of a compact low-cost Stokes lens for astigmatism compensation in optical instruments

2020

Variable power cross-cylinder lenses (or Stokes lenses) have been widely known in the literature for decades. In this paper, we describe how to build a low-cost Stokes lens and discuss its calibration and its application to two significant cases. The construction is in virtue of a phoropter’s Risley prism mount for assembling a couple of equal but opposite sign cylindrical lenses (we have selected ± 1.50 D). Thus, variable astigmatic power is achieved by relative rotation of the lenses in opposite directions, and the resulting astigmatic axis is defined by the global rotation of the device. Calibration measurements are performed using an automatic lensmeter (Topcon CL-300) and an aberromete…

Aberration compensation for objective phase curvature in phase holographic microscopy: comment

2014

In a recent Letter by Seo et al. [Opt. Lett. 37, 4976 (2012)], the numerical correction of the quadratic phase distortion introduced by the microscope objective in digital holographic microscopy (DHM) has been presented. In this comment, we would like to draw to the attention of the authors and the readers in general that this approach could not be the optimal solution for maintaining the accuracy of the quantitative phase via DHM. We recall that the use of telecentric imaging systems in DHM simplifies the numerical processing of the phase images and produces more accurate measurements.

Resolution improvements in integral microscopy with Fourier plane recording

2016

Abstract: Integral microscopes (IMic) have been recently developed in order to capture the spatial and the angular information of 3D microscopic samples with a single exposure. Computational post-processing of this information permits to carry out a 3D reconstruction of the sample. By applying conventional algorithms, both depth and also view reconstructions are possible. However, the main drawback of IMic is that the resolution of the reconstructed images is low and axially heterogeneous. In this paper, we propose a new configuration of the IMic by placing the lens array not at the image plane, but at the pupil (or Fourier) plane of the microscope objective. With this novel system, the spa…

Shift-variant digital holographic microscopy: inaccuracies in quantitative phase imaging

2013

Inaccuracies introduced in quantitative phase digital holographic microscopy by the use of nontelecentric imaging systems are analyzed. Computer modeling of the experimental result shows that even negligible errors in the radius and center of curvature of the numerical compensation needed to get rid of the remaining quadratic phase factor introduce errors in the phase measurements; these errors depend on the position of the object in the field-of-view. However, when a telecentric imaging system is utilized for the recording of the holograms, the numerical modeling and experimental results show the shift-invariant behavior of the quantitative-phase digital holographic microscope.

Superresolution digital holographic microscopy for three-dimensional samples.

2009

An approach that allows superresolution imaging of three-dimensional (3-D) samples by numerical refocusing is presented in the field of digital holographic microscopy. Based on the object's spectrum shift produced by tilted illumination, we present a time multiplexing superresolved approach to overcome the Abbe's diffraction limit. The proposed approach uses a microscope in a Mach-Zehnder interferometric architecture with the particularity that the output plane does not coincide with the image plane. Thus, a set of off-axis non-image plane holograms are sequentially recorded for every tilted beam used in the illumination stage. After that and by using simple digital post-processing and nume…