Search results for "SEPARATION"

Identification and purification of human erythroid progenitor cells by monoclonal antibody to the transferrin receptor (T� 67)

1988

Anti-TU 67 is a murine monoclonal antibody that recognizes the transferrin receptor. With respect to hematopoietic cells TU 67 is expressed by human multipotent colony-forming cells (CFU-Mix), erythroid progenitor cells (BFU-E and CFU-E) and a fraction of granulocyte/monocyte colony forming cells, but is not expressed by mature hematopoietic cells including erythrocytes, platelets, lymphocytes, and peripheral blood myeloid cells. The TU 67-positive fraction of normal bone marrow, separated by fluorescence-activated cell sorting (FACS) or immune rosettes, contained 87% of the erythroid progenitor cells. Erythroid progenitor cells were enriched up to 50-fold by using a combination of monoclon…

Separaxion anxiety in pediatric migraine without aura: A pilot study

2017

Background: Separation anxiety (SA) can be defined as the fear reaction and protest manifested by children when the main caregivers move away from him/her or in front of unfamiliar person. SA near eight months may be considered as an important and normal phase of the correct and typical social neurodevelopment. Aims of the present pilot study is assessing the prevalence of separation anxiety in a population of patients with migraine without aura (MwA). Materials and methods: 119 children (69 males) suffering from MwA (mean age 1.78 ± 7:59) were consecutively recruited. The control population consisted of 231 (114 males) healthy subjects similar for age (7.64 ± 1:34; p = 0.768) and gender (p…

Design Considerations of Transverse Flux Generator to Sea Wave Energy

2014

In this paper we study the possibility to use a transverse flux linear generator (TFG) because transverse flux technology presents the highest force density per volume index among the iron based electrical machines. This paper is organized as follows an introduction to describe "state of the art" of WEC's and TFG, in the Section II we find a summary description of the marine condition of the chosen site; in the Section III there is an the analytical procedure for geometric and magnetic design of device (chosen material, length, etc) and in the Section IV the simulation of generator. Further defined the design of the generator, the machine was designed and analyzed through the use of a 3D FE…

Semi-blind Independent Component Analysis of functional MRI elicited by continuous listening to music

2013

This study presents a method to analyze blood-oxygen-level-dependent (BOLD) functional magnetic resonance imaging (tMRI) signals associated with listening to continuous music. Semi-blind independent component analysis (ICA) was applied to decompose the tMRI data to source level activation maps and their respective temporal courses. The unmixing matrix in the source separation process of ICA was constrained by a variety of acoustic features derived from the piece of music used as the stimulus in the experiment. This allowed more stable estimation and extraction of more activation maps of interest compared to conventional ICA methods.

Analysis of primary aliphatic short-chain monoamines by LC in water samples

2003

Abstract Several derivatization procedures with o-phthaldialdehyde-N-acetylcysteine (OPA-NAC) were compared for a rapid analysis of primary aliphatic short-chain monoamines in water samples by HPLC using a LiChorospher analytical separation column (100RP18 125 mm ×4  mm i.d., 5 μm). Both the solution and the solid-support assisted off-line derivatization on C18 SPE cartridges were inadequate options because of beginning degradation processes of the instable isoindol derivatives during their transfer to the analytical column. This problem was precluded with the on-column or solid-support assisted on-line derivatization. In the last mentioned procedure, the derivatization took place in a Hype…

POROUS SCAFFOLDS BASED ON PLLA/FUNCTIONALISED POLYMERS BLENDS PRODUCED BY THERMALLY INDUCED PHASE SEPARATION

2014

Solution-Based Processing for Scaffold Fabrication in Tissue Engineering Applications: A Brief Review

2021

The fabrication of 3D scaffolds is under wide investigation in tissue engineering (TE) because of its incessant development of new advanced technologies and the improvement of traditional processes. Currently, scientific and clinical research focuses on scaffold characterization to restore the function of missing or damaged tissues. A key for suitable scaffold production is the guarantee of an interconnected porous structure that allows the cells to grow as in native tissue. The fabrication techniques should meet the appropriate requirements, including feasible reproducibility and time- and cost-effective assets. This is necessary for easy processability, which is associated with the large …

Synthesis, characterization and foaming of PHEA-PLLA, a new graft copolymer for biomedical engineering

2014

Abstract In this study a chemical grafting procedure was set up in order to link high molecular weight poly L-lactic acid (PLLA) chains to the hydrophilic α,β-poly(N-2-hydroxyethyl)-DL-aspartamide (PHEA) backbone. A graft copolymer named PHEA-g-PLLA (or simply PHEA–PLLA) was obtained bearing a degree of derivatization of 1.0 mol.% of PLLA as grafted chain. This new hybrid derivative offers both the opportune crystallinity necessary for the production of scaffolds trough a thermally induced phase separation (TIPS) technique and the proper chemical reactivity to perform further functionalizations with bio-effectors and drugs. PHEA–PLLA porous scaffolds for tissue engineering applications were…

Preparation and characterization of PLLA-HA scaffolds for bone tissue engineering

2013

In this work, the possibility to produce composite - Poly-L-lactic acid (PLLA) and Hydroxyapatite (HA) - porous scaffolds via Thermally Induced Phase Separation for bone tissue engineering applications was investigated. Several PLLA/HA ratios were tested (70/30, 50/50, 30/70 and 20/80 wt/wt) and the as-obtained scaffolds were characterized via Scanning Electron Microscopy (SEM), Wide Angle X-Ray Diffraction (WAXD) and compression test. The results showed that the presence of HA does not influence the phase separation process. Morphological analysis revealed an open structure with interconnected pores and HA embedded in the polymer matrix. This evidence was confirmed by WAXD analysis; where …

A COMPOSITE PLLA SCAFFOLD FOR REGENERATION OF COMPLEX TISSUES

2010

A composite biodegradable scaffold incorporating an integrated network of synthetic blood vessels was designed and prepared, in line with the requirements of a scaffold effectively supporting the regeneration of highly vascularized tissues. In other words, this composite scaffold should allow the regeneration of complex injured tissue (e.g. dermis) and, at the same time, favour the development of a vascular network on its inner, i.e. a 3D polymeric scaffolds embedding synthetic blood vessel-like structures for nutrient supply and metabolite removal. PLLA assures a high degree of biocompatibility and a low level of inflammation response upon implantation, while the embedded tubular vessel-li…