Search results for "Scaffold"

An approach to a biomimetic bone scaffold: increased expression of BMP-2 and of osteoprotegerin in SaOS-2 cells grown onto silica-biologized 3D print…

2012

Three-dimensional printed (3D printed) bone material is needed to close the shortage and to avoid the potential health risks associated with autografts and allografts, in the treatment of bone fractures/nonunions or bone trauma. Here we describe the fabrication of 3D printed scaffold, initially prepared form Ca-sulfate that has been impregnated/biologized with Ca-phosphate or with silica. The 3D printed grids had a size mesh of 200 μm; the chemical composition was determined by energy dispersive X-ray spectroscopy or conventional chemical analysis. Using human SaOS-2 cells (human osteogenic cells) it is shown that both the Ca-sulfate, and the Ca-phosphate or the silica impregnated Ca-sulfat…

Coronary Stent Strut Fractures: Classification, Prevalence and Clinical Associations

2021

Introduction. The frequency, characteristics and clinical implications of Strut fractures (SFs) remain incompletely understood. Methods and results. A total of 185 (160 patients) newer-generation drug-eluting stents (DES) were imaged. SFs were found in 21 DES (11.4%) and were classified in four patterns: one single stacked strut (41%)

Opettajan ohjauksen ja vuorovaikutuksen antaman tuen merkitys oppilaiden rakentaessa mallia magnetismista

2013

A Continuous Pore Size Gradient PLLA Scaffold For Osteochondral Regeneration

2016

Osteochondral (OC) scaffold-based regenerative approaches in the joint are challenging since the scaffold must provide mechanical strength while also mimicking the local cartilage and bone microenvironments. Thermally Induced Phase Separation (TIPS) can produce scaffolds with a wide range of pore size morphologies/distributions. Here, we produced by TIPS a poly-L-lactide (PLL A) scaffold with a continuous pore size gradient along the sample thickness, from ~70μm diameter on one side to ~200 μm diameter on the opposite surface.

Melt Processed PCL/PEG Scaffold with Discrete Pore Size Gradient for Selective Cellular Infiltration

2016

In order to develop scaffold able to mimic the natural gradient properties of tissues, biphasic and triphasic approaches were adopted. In this work, polycaprolactone/polyethylene glycol (PCL/PEG) scaffolds were prepared by using a combination of melt mixing and selective leaching without harmful solvents. The method permitted to develop three-layer scaffolds with high control of porosity and pore size. The mechanical properties were evaluated under physiological condition in order to simulate the real conditions of work. Co-culture of osteoblastic and fibroblastic mice cells were carried out in order to study the differential cellular permeation through the different pore size layers.

Three-layered porous device in PCL/PEG blend for interface tissue engineering

2016

Tissue interfaces, such as cartilage-to-bone, exhibit anisotropic structural properties, which gradually vary from one tissue to another. Consequently a regenerative scaffold designed for interface tissues should exhibit a gradient in composition, structure and mechanical features, mimicking those of the native zones. In particular, the architecture of pores plays a central role. Indeed, a biomedical implant should be designed with porosityand pore size gradients simulating the structure of the two interface tissues. One of the most common techniques to prepare porous scaffolds is the particulate leaching method, which involves the selective leaching of a mineral or organic compound as poro…

Teaching Is a Story Whose First Page Matters : Teacher Counselling as Part of Teacher Growth

2022

The starting point of the study is the work counselling process taking place in the induction phase of the students’ teaching career. The work counselling process consists of activities that support and strengthen the students’ teacher hood and their primary function as teachers by helping them to analyse their work and to attach to working life as newly graduated teachers. In this study, we use contextualisation and storytelling in the frameworks of positive pedagogy and systems theory to reflect on the students’ growth process as teachers. We also acknowledge the importance of Vygotsky’s zone of proximal development and, related to that, timely learning support (scaffolding) and a solutio…

Micro – Meso scale based biomechanical models to assist the design and fabrication of scaffolds for Cardiac Tissue Regeneration

2011

Questo lavoro presenta una strategia di modellazione strutturale basata su dati di micro-architettura finalizzata a: (A) guidare fabbricazione e progetto di scaffold per ingegneria di tessuto, (B) studiare la risposta cellulare meccanica alle deformazioni strutturali. Applicazioni cliniche ideali per la metodologia proposta sono le valvole cardiache e il patch di miocardio. Custom software di analisi d’immagine e’ stato utilizzato per caratterizzare la morfologia dei campioni. Le topologie dei materiali individuate sono state impiegate per generare equivalenti modelli a rete. Il comportamento meccanico alla macro scala e’ stato studiato mediante prove di carico biassiale. Questa messe speri…

Multilayer porous PLA scaffolds for tissue engineering applications

2013

Rat Cardiac progenitor cells and their application in cell therapy

2015

cells and the subsequent heart failure. When the pharmacological approach no longer complies with the disease evolution, organ transplantation appears to be the only treatment able to rescue the patient life. Cell therapy promises to be clinically efficient and would allow circumventing many limitations of organ transplantation, such as organ low availability, major surgical procedures, high costs and longterm immunosuppression [1]. We designed porous Poly-Lactic Acid (PLLA) and Fibroin scaffolds to deliver CPCs in the heart, we isolated and characterized CPCs for the expression of c-Kit, MDR-1 and Sca-1 by flow cytometry, we tested their degree of differentiation in vitro studying the expr…