Search results for "Spheroid"

Sur la fonction des « sphéroïdes » en tôle de l’Age du bronze

2013

La fonction des quatre sphéroïdes en tôle de bronze de France et de Suisse connus, souvent décrits, n’a jamais été établie. En raison de leur mode de façonnage, de leur technique de montage et du style de leur décoration, ils sont rapprochés ici des sphéroïdes italiens qui sont généralement complets. Ces derniers, découverts en général dans des sépultures, étant interprétés comme des encensoirs, il est proposé de considérer comme tels, avec des réserves du fait de leur état fragmentaire, les exemplaires de France et de Suisse. Ces récipients, datés en France du Bronze final IIIb, connaissent en Italie une évolution qui se prolonge jusqu’au début du VIIe s. av. J.-C. Although the four known …

Adipose-derived stem cells

2013

SPECIAL EDITORIAL

Synthesis and biological evaluation of new imidazo[2,1-b][1,3,4]thiadiazole derivatives: as anticancer and antibiofilm agents, and preclinical invest…

2020

MiR-221 promotes stemness of breast cancer cells by targeting DNMT3b

2016

// Giuseppina Roscigno 1, 2 , Cristina Quintavalle 1, 2 , Elvira Donnarumma 3 , Ilaria Puoti 1 , Angel Diaz-Lagares 4 , Margherita Iaboni 1 , Danilo Fiore 1 , Valentina Russo 1 , Matilde Todaro 5 , Giulia Romano 6 , Renato Thomas 7 , Giuseppina Cortino 7 , Miriam Gaggianesi 5 , Manel Esteller 4 , Carlo M. Croce 6 , Gerolama Condorelli 1, 2 1 Department of Molecular Medicine and Medical Biotechnology, “Federico II” University of Naples, Naples, Italy 2 IEOS-CNR, Naples, Italy 3 IRCCS-SDN, Naples, Italy 4 Epigenetic and Cancer Biology Program (PEBC) IDIBELL, Hospital Duran I Reynals, Barcelona, Spain 5 Department of Surgical and Oncological Sciences, Cellular and Molecular Pathophysiology Lab…

Glucose Diffusion Coefficients Determined from Concentration Profiles in Emt6 Tumor Spheroids Incubated in Radioactively Labeled L-Glucose

1994

In order to theoretically assess tissue energetic status, conditions for substance exchange need to be known. One group of parameters important in this context are diffusion coefficients of nutrients and metabolic waste products which may be assessed by incubating spheroids in a medium containing tracer amounts of the radioactively labeled substance in question, for a defined period of time. In previous studies, the overall amount of 14C-labeled substance taken up by the spheroids was measured by scintillation counters (e.g.[1]), or the concentration of 3H-labeled substance in the spheroid center was determined by autoradiography and grain counting (e.g. [4]). From a number of such measurem…

Development of an in vitro neuroblastoma 3D model and its application for sterigmatocystin-induced cytotoxicity testing

2021

Abstract Given the increasing importance of establishing better risk assessments for mycotoxins, novel in vitro tools for the evaluation of their toxicity are mandatory. In this study, an in vitro 3D spheroid model from SH-SY5Y cells, a human neuroblastoma cell line, was developed, optimized and characterized to test the cytotoxic effects caused by the mycotoxin sterigmatocystin (STE). STE induced a concentration- and time-dependent cell viability decrease in spheroids. Spheroids displayed cell disaggregation after STE exposure, increasing in a dose-dependent manner and over time. STE also induced apoptosis as confirmed by immunofluorescence staining and Western blot. Following the decrease…

Fast Computation by Subdivision of Multidimensional Splines and Their Applications

2016

We present theory and algorithms for fast explicit computations of uni- and multi-dimensional periodic splines of arbitrary order at triadic rational points and of splines of even order at diadic rational points. The algorithms use the forward and the inverse Fast Fourier transform (FFT). The implementation is as fast as FFT computation. The algorithms are based on binary and ternary subdivision of splines. Interpolating and smoothing splines are used for a sample rate convertor such as resolution upsampling of discrete-time signals and digital images and restoration of decimated images that were contaminated by noise. The performance of the rate conversion based spline is compared with the…

Interrelationship Among Morphology, Metabolism, and Proliferation of Tumor Cells in Monolayer and Spheroid Culture

1989

Previous investigations have indicated a positive correlation between the proliferative and metabolic activities of tumor cells in monolayer culture (Freyer et al., 1984; Freyer and Sutherland, 1985; Walenta and Mueller-Klieser, 1987). On the other hand, no difference in the local oxygen consumption has been found between highly proliferating outer cell areas and non-proliferating inner cellular regions in multicellular tumor spheroids (Mueller-Klieser, 1984, 1987). Therefore, the interrelationship among metabolism, proliferation, and cellular morphology was investigated systematically in tumor cells both in monolayer and spheroid culture.

Alginate encapsulation improves viability and integrity of cryopreserved pancreatic islets and multicellular spheroids: combined fluorescence, scanni…

2007

Extended abstract of a paper presented at MC 2007, 33rd DGE Conference in Saarbrücken, Germany, September 2 – September 7, 2007

Cytotoxicity of natural products and derivatives toward MCF-7 cell monolayers and cancer stem-like mammospheres

2015

Abstract Although cancer stem-like cells (CSCs) are rare, they can enter a non-proliferative or dormant state and resist therapy. Furthermore, quiescent CSCs are responsible for metastases that can appear after curative surgical treatment of a primary tumor. Because of drug resistance of CSCs, the development of novel therapies is urgently required that specifically target CSCs. Purpose The aim of the present study was to investigate the potential of a panel of natural products and derivatives to inhibit CSC-enriched mammospheres of MCF-7 breast cancer cells. Methods CD44high/CD24low cells were identified by flow cytometry and maintained as mammospheres. As a control, we used two clinically…