Search results for "TERMINATION"

Deciphering the Nonsense Readthrough Mechanism of Action of Ataluren: An in Silico Compared Study

2019

Ataluren was reported to suppress nonsense mutations by promoting the readthrough of premature stop codons, although its mechanism of action (MOA) is still debated. The likely interaction of Ataluren with CFTR-mRNA has been previously studied by molecular dynamics. In this work we extended the modeling of Ataluren's MOA by complementary computational approaches such as induced fit docking (IFD), quantum polarized ligand docking (QPLD), MM-GBSA free-energy calculations, and computational mutagenesis. In addition to CFTR-mRNA, this study considered other model targets implicated in the translation process, such as eukaryotic rRNA 18S, prokaryotic rRNA 16S, and eukaryotic Release Factor 1 (eRF…

2019

Abstract This paper presents an index that measures reviewer contribution to editorial processes of scholarly journals. Following a metaphor of ranking algorithms in sports tournaments, we created an index that considers reviewers on different context-specific dimensions, i.e., report delivery time, the length of the report and the alignment of recommendations to editorial decisions. To test the index, we used a dataset of peer review in a multi-disciplinary journal, including 544 reviewers on 606 submissions in six years. Although limited by sample size, the test showed that the index identifies outstanding contributors and weak performing reviewers efficiently. Our index is flexible, cont…

Unconventional monetary policy reaction functions: evidence from the US

2020

Abstract We specify unconventional monetary policy reaction functions for the Fed using linear and nonlinear econometric frameworks. We find that nonstandard policy measures are largely driven by the dynamics of inflation and the output gap, with the effect being particularly strong during QE rounds. Moreover, we uncover the presence of asymmetry and regime dependence in central bank’s actions since the global financial crisis, especially concerning the response of the term spread and the shadow short rate to the growth rate of central bank reserves. From a policy perspective and given the lack of a systematic response of monetary policy to asset price growth in nonstandard times, our findi…

Regional Susceptibility in VCD Spectra to Dynamic Molecular Motions

2018

Experimental and theoretical studies of the vibrational circular dichroism (VCD) spectrum of 3-methyl-1-(methyldiphenlsilyl)-1-phenylbutan-1-ol, whose absolute configuration is key to elucidating the Brook rearrangement of tertiary benzylic α-hydroxylsilanes, are presented. It is found that the entire OH-bending region in this spectrum—a region that provides important marker bands—cannot be reproduced at all by standard theoretical approaches even though other regions are well described. Using a novel approach to disentangle contributions to the rotational strength of these bands, internal coordinates are identified that critically influence the appearance of this part of the spectrum. We s…

Chemical oxidation and reduction of hexachlorocyclohexanes: A review

2019

Abstract Lindane (γ-hexachlorocyclohexane) and its isomers (HCH) are some of the most common and most easily detected organochlorine pesticides in the environment. The widespread distribution of lindane is due to its use as an insecticide, accompanied by its persistence and bioaccumulation, whereas HCH were disposed of as waste in unmanaged landfills. Unfortunately, certain HCH (especially the most reactive ones: γ- and α-HCH) are harmful to the central nervous system and to reproductive and endocrine systems, therefore development of suitable remediation methods is needed to remove them from contaminated soil and water. This paper provides a short history of the use of lindane and a descri…

Implant insertion torque value in immediate loading : a retrospective study

2019

Background The aim of this study is to verify if the Insertion Torque Value (ITV) of 32 Ncm for immediate loading protocol (ILP), as indicated by literature, is still, with the advance in implant research, a real significant cut-off for long-term implant survival. Material and Methods In this retrospective study, data from 224 patients that during three years of clinical practice, were submitted to the insertion of 322 implants with immediate loading protocol, have been recorded, pooled and analyzed. Data were organized based on Insertion Torque Value (ITV): > 32 Ncm (CG) and 32 Ncm are still characterized by a lower crestal bone resorption, there are no statistically significant difference…

Determination and separation of diarylheptanoids from alder growing in Latvia

2015

The composition of diarylhepatnoids fraction isolated from bark of two alder species (grey alder and black alder) was studied. The efficiency of three extraction methods used for isolation of diarylhepatnoids from alder bark was compared. Two diarylhepatnoids: 1,7-bis-(3,4-dihydroxyphenyl)-heptan- 3-one-5-O-β-D-xylopyronoside (oregonin) and 1,7-bis-(3,4-dihydroxyphenyl)-3-hydroxyheptane-5-O-β-D- xylopyranoside were isolated from the bark of grey alder. The phenolic components of the extracts were analyzed by high-performance liquid chromatography (HPLC). Quantitative determination of oregonin was performed using an internal standard method. The results obtained show that alder barks are ric…

Portrait of inflammatory response to ionizing radiation treatment

2015

Ionizing radiation (IR) activates both pro-and anti-proliferative signal pathways producing an imbalance in cell fate decision. IR is able to regulate several genes and factors involved in cell-cycle progression, survival and/or cell death, DNA repair and inflammation modulating an intracellular radiation-dependent response. Radiation therapy can modulate anti-tumour immune responses, modifying tumour and its microenvironment. In this review, we report how IR could stimulate inflammatory factors to affect cell fate via multiple pathways, describing their roles on gene expression regulation, fibrosis and invasive processes. Understanding the complex relationship between IR, inflammation and …

The Role of Agency Theory and Perceived Goal Divergence in IS Continuance: A Replication and Extension Study

2016

Past literature recognizes the power of the well-established information systems continuance theory (ISCT) to explain information systems (IS) continuance. In this study, we integrate constructs from two additional perspectives and discuss their interdependencies with ISCT. We argue that the promising framework proposed by Sorebo et al. [1], which extends ISCT with self-determination theory (SDT), can increase its managerial relevance by adding the variables of users' perceptions of goal divergence and risk aversion, selected from agency theory. The empirical results support Sorebo et al.'s main findings and strongly support the impact of perceived goal divergence on e-learning continuance …

Determinación del colesterol en leche humana: una alternativa a los métodos cromatográficos

2015

Introduction: human milk (HM) is considered the best option for feeding healthy infants. Cholesterol (CHOL) is important for proper development of the nervous system, and for hormone and vitamin synthesis in growing infants. Breastfeeding and dietary CHOL intake during infancy have been suggested to affect blood lipid levels and the risk of cardiovascular disease in adulthood. Gas chromatography is the technique most widely used to determine CHOL in HM. Chromatographic methods are specific for the determination of CHOL and other sterols present in HM, but are extremely time consuming, and the costs and equipment requirements mean that they are not accessible to all laboratories. Aim: the pr…