Search results for "Techniques"
showing 10 items of 4426 documents
Enhancement of hepatitis C virus RNA replication by cell culture-adaptive mutations.
2001
ABSTRACT Studies of the Hepatitis C virus (HCV) replication cycle have been made possible with the development of subgenomic selectable RNAs that replicate autonomously in cultured cells. In these replicons the region encoding the HCV structural proteins was replaced by the neomycin phosphotransferase gene, allowing the selection of transfected cells that support high-level replication of these RNAs. Subsequent analyses revealed that, within selected cells, HCV RNAs had acquired adaptive mutations that increased the efficiency of colony formation by an unknown mechanism. Using a panel of replicons that differed in their degrees of cell culture adaptation, in this study we show that adaptive…
Viral and cellular determinants of hepatitis C virus RNA replication in cell culture.
2003
Studies on the replication of hepatitis C virus (HCV) have been facilitated by the development of selectable subgenomic replicons replicating in the human hepatoma cell line Huh-7 at a surprisingly high level. Analysis of the replicon population in selected cells revealed the occurrence of cell culture-adaptive mutations that enhance RNA replication substantially. To gain a better understanding of HCV cell culture adaptation, we characterized conserved mutations identified by sequence analysis of 26 independent replicon cell clones for their effect on RNA replication. Mutations enhancing replication were found in nearly every nonstructural (NS) protein, and they could be subdivided into at …
Tumor targeting of baculovirus displaying a lymphatic homing peptide.
2008
Background Tumor-associated cells and vasculature express attractive molecular markers for site-specific vector targeting. To attain tumor-selective tropism, we recently developed a baculovirus vector displaying the lymphatic homing peptide LyP-1, originally identified by ex vivo/in vivo screening of phage display libraries, on the viral envelope by fusion to the transmembrane anchor of vesicular stomatitis virus G-protein. Methods In the present study, we explored the specificity and kinetics of viral binding and internalization as well as in vivo tumor homing of the LyP-1 displaying virus to elucidate the applicability of baculovirus for targeted therapies. Results We demonstrated that th…
The ALICE experiment at the CERN LHC
2008
Journal of Instrumentation 3(08), S08002 (2008). doi:10.1088/1748-0221/3/08/S08002
Procedures for study of teaching practices. Literature Review
2012
Interest in studying teaching practices has increased, because of the need to evaluate teachers and dissatisfaction with the usual ways of doing it. Recent approaches with Value-Added Models, based on students’ results on achievement tests do not seem satisfactory. The article is based on a review of literature and classifies the approaches to practices in three groups: instruments based on information given by teachers; observation protocols; and approaches based on the analysis of products of the practices. Specific tools are described and advantages and disadvantages of the three approaches are discussed. El interés por las prácticas docentes ha aumentado, en parte por la necesidad de ev…
Vārdu krājumu novērtējums ar dažādu testēšanu paņēmienu izmantošanu
2016
Darbā tika pētītas vārdu krājuma testēšanas paņēmienu priekšrocības un trūkumi un visefektīvākais un drošākais paņēmiens vārdu krājuma pārbaudei mācību apstākļos. Pētījuma rezultāti spēj veicināt izvēli par labu konkrētam testēšanas paņēmienam, saskaņā ar novērtēšanas veidu un mērķi, tēmu, mērķauditoriju un vidi. Darba teorētiskā daļa ir balstīta uz Arthur Hughes (2003), Scott Thornbury (2002), John Read (2000) un Penny Ur (1999) teorijas. Gadījuma metodes ietvaros tika vadīta mācību nodarbība, pēc kuras studentu zināšanas tika novērtētas ar autores izstrādāto testa palīdzību. Kvantitatīva analīze tika pielietota testa rezultātu noteikšanā un uzticamības mērīšanā, turklāt kvalitatīva analīz…
Study of vocalists' use of emotions in performance preparation
2015
Musicians and educators understand the importance of expressivity in vocal performance. Despite the acceptance of expressivity as a crucial element in musical performance, educators have reported addressing it only cursorily or not addressing it at all when preparing for a performance. For these reasons, this research has focused on techniques used by trained musicians when preparing a performance, to be adapted and applied for use by teachers with their large vocal ensembles. Participants in this study were enrolled in vocal / operatic performance programs at universities in the United States, Ireland, Finland, and Hong Kong. Their progress was tracked throughout the semester by three ques…
Highly sensitive olfactory biosensors for the detection of volatile organic compounds by surface plasmon resonance imaging
2018
International audience; Nowadays, monitoring of volatile organic compounds (VOCs) is very important in various domains. In this work, we aimed to develop sensitive olfactory biosensors using odorant binding proteins (OBPs) as sensing materials. Three rat OBP3 derivatives with customized binding properties were designed and immobilized on the same chip for the detection of VOCs in solution by surface plasmon resonance imaging (SPRi). We demonstrated that the proteins kept their binding properties after the immobilization under optimized conditions. The obtained olfactory biosensors exhibited very low limits of detection in both concentration (200pM of beta-ionone) and in molecular weight of …
Spatially resolved SO2 flux emissions from Mt Etna
2016
Abstract We report on a systematic record of SO2 flux emissions from individual vents of Etna volcano (Sicily), which we obtained using a permanent UV camera network. Observations were carried out in summer 2014, a period encompassing two eruptive episodes of the New South East Crater (NSEC) and a fissure‐fed eruption in the upper Valle del Bove. We demonstrate that our vent‐resolved SO2 flux time series allow capturing shifts in activity from one vent to another and contribute to our understanding of Etna's shallow plumbing system structure. We find that the fissure eruption contributed ~50,000 t of SO2 or ~30% of the SO2 emitted by the volcano during the 5 July to 10 August eruptive inter…
Experimental Validation of Maximum Constant Boost Control and Switching Frequency Optimal for three-phase Quasi-Z-Source Converters
2020
This paper presents a modified modulation scheme for quasi-Z-Source converters, based on the Maximum Constant Boost Control (MCBC) concept and Switching Frequency Optimal (SFO) as reference signal. The effectiveness of the proposed technique is validated by comparing the obtained results (in terms of AC harmonic content and voltage stress) with those carried out from traditional modulation schemes. For this reason, a test bench has been assembled and the benefits of the SFO MCBC modulation scheme have been valuated.