Search results for "Time-of-flight"

showing 10 items of 61 documents

Conceptual study of a heavy-ion-ERDA spectrometer for energies below 6 MeV

2017

Abstract Elastic recoil detection analysis (ERDA) is a well established technique and it offers unique capabilities in thin film analysis. Simultaneous detection and depth profiling of all elements, including hydrogen, is possible only with time-of-flight ERDA. Bragg ionization chambers or Δ E - E detectors can also be used to identify the recoiling element if sufficiently high energies are used. The chief limitations of time-of-flight ERDA are the beam induced sample damage and the requirement of a relatively large accelerator. In this paper we propose a detector setup, which could be used with 3 MeV to 6 MeV medium heavy beams from either a single ended accelerator (40Ar) or from a tandem…

010302 applied physicsNuclear and High Energy PhysicsERDASpectrometerta114Physics::Instrumentation and DetectorsChemistryDetectortime-of-flight01 natural sciencesNuclear physicsPelletronElastic recoil detectionTime of flightvetyIonizationhydrogen0103 physical sciencesIonization chamber010306 general physicsInstrumentationBeam (structure)Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms
researchProduct

Simulations on time-of-flight ERDA spectrometer performance

2016

The performance of a time-of-flight spectrometer consisting of two timing detectors and an ionization chamber energy detector has been studied using Monte Carlo simulations for the recoil creation and ion transport in the sample and detectors. The ionization chamber pulses have been calculated using Shockley-Ramo theorem and the pulse processing of a digitizing data acquisition setup has been modeled. Complete time-of-flight–energy histograms were simulated under realistic experimental conditions. The simulations were used to study instrumentation related effects in coincidence timing and position sensitivity, such as background in time-of-flight–energy histograms. Corresponding measurement…

010302 applied physicsPhysicsta114SpectrometerPhysics::Instrumentation and Detectorsbusiness.industryInstrumentationMonte Carlo methodDetector7. Clean energy01 natural sciencesMonte Carlo simulationsNuclear physicsTime of flightRecoilOpticsData acquisitiontime-of-flight spectrometers0103 physical sciencesIonization chambersimulations010306 general physicsbusinessInstrumentationReview of Scientific Instruments
researchProduct

Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS.

2017

We developed an easy MALDI-TOF MS-based assay to identify microorganisms directly from thioglycolate broth. A total of 101 positive thioglycolate broths inoculated with 15 different kinds of samples were evaluated. In 91 samples (90.1%), direct MALDI-TOF MS identifications were the same as those obtained after conventional laboratory procedures including subcultures. In 10 samples misidentified by direct processing, yeasts or mixed cultures grew in the thioglycolate subcultures, or high cellular debris hampered a correct analysis. This rapid method can provide a fast, clinically- relevant species-level identification without disturbing the daily workflow in clinical microbiology laboratorie…

0301 basic medicinePhysiologyMicroorganismlcsh:MedicinePathology and Laboratory MedicineNervous SystemMass SpectrometryAnalytical Chemistrychemistry.chemical_compoundSpectrum Analysis TechniquesMicrobial PhysiologyCandida albicansMedicine and Health SciencesMatrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometrylcsh:ScienceCerebrospinal FluidMultidisciplinaryChemistryMicrobial Growth and DevelopmentClinical Laboratory SciencesBody FluidsClinical microbiologyChemistryClinical LaboratoriesThioglycolatesPhysical SciencesAnaerobic bacteriaAnatomyCellular DebrisResearch ArticleClinical PathologyThioglycolate broth030106 microbiologyAnaerobic BacteriaResearch and Analysis MethodsMicrobiology03 medical and health sciencesSigns and SymptomsDiagnostic MedicineGram Negative BacteriaLaboratory methodsChromatographyBacteriaBacterial Growthlcsh:ROrganismsBiology and Life SciencesBacteriologyCulture MediaMatrix-assisted laser desorption/ionizationAbscessesClinical MicrobiologySpectrometry Mass Matrix-Assisted Laser Desorption-Ionizationlcsh:QLaboratoriesDevelopmental BiologyPLoS ONE
researchProduct

Metabolic signatures across the full spectrum of non-alcoholic fatty liver disease.

2022

Funder: European Commission

ALTtype 2 diabetes mellitusROC receiving operator characteristicaspartate aminotransferaseHSDLDL low-density lipoproteinUHPLC ultrahigh-performance liquid chromatographyROCHCCNon-alcoholic steatohepatitisGCPCANASHGastroenterology2-HB 2-hydroxybutanoic acid; 3-HB 3-hydroxybutanoic acid; ALT alanine aminotransferase; AST aspartate aminotransferase; CE cholesterol ester; Cer ceramide; FFA free fatty acid; FLIP Fatty Liver Inhibition of Progression; Fibrosis; GC gas chromatography; HCC hepatocellular carcinoma; HSD honest significant difference; LC lipid cluster; LDL low-density lipoprotein; LM lipid and metabolite; LMC lipid metabolite and clinical variable; LPC lysophosphatidylcholine; Lipidomics; Mass spectrometry; Metabolomics; NAFL non-alcoholic fatty liver; NAFLD non-alcoholic fatty liver disease; NAS NASH activity score; NASH non-alcoholic steatohepatitis; NIDDK NASH-CRN National Institute of Digestive Diseases and Kidney NASH Clinical Research Network; NRR non-rejection rate; Non-alcoholic steatohepatitis; PC(O) ether PC; PC phosphatidylcholine; PCA principal component analysis; PE phosphatidylethanolamine; QTOFMS quadrupole-time-of-flight mass spectrometry; ROC receiving operator characteristic; SAF steatosis activity and fibrosis; SM sphingomyelin; T2DM type 2 diabetes mellitus; TG triacylglycerol; UHPLC ultrahigh-performance liquid chromatographySAFSAF steatosis activity and fibrosisLM lipid and metabolitehonest significant differenceHSD honest significant differenceTG triacylglycerolnon-rejection ratecholesterol esterPCPEGC gas chromatographyfree fatty acidFLIPNASH non-alcoholic steatohepatitisNIDDK NASH-CRN National Institute of Digestive Diseases and Kidney NASH Clinical Research NetworkBIOMARKERST2DMPE phosphatidylethanolamineLDLlipidNAFLDFFA free fatty acid2-HBMetabolomicsNAFL non-alcoholic fatty liverLMCphosphatidylcholineScience & TechnologySM sphingomyelinGastroenterology & HepatologyMass spectrometryactivitynutritional and metabolic diseasesT2DM type 2 diabetes mellitusACIDSreceiving operator characteristicdigestive system diseasesquadrupole-time-of-flight mass spectrometryLC lipid clusterlow-density lipoproteinNAS2-HB 2-hydroxybutanoic acidNAS NASH activity scoreQTOFMSether PCNRRSCORING SYSTEMprincipal component analysisgas chromatographyLC2-hydroxybutanoic acidPROGRESSIONAST aspartate aminotransferaseLMPC phosphatidylcholinePC(O)MARKERSUHPLCsteatosisTOOLImmunology and AllergyINSULIN-RESISTANCECerSMFatty Liver Inhibition of Progressionhepatocellular carcinoma2-HB 2-hydroxybutanoic acid NIDDK NASH-CRN National Institute of Digestive Diseases and Kidney NASH Clinical Research Network NRR non-rejection rate Non-alcoholic steatohepatitis PC(O) ether PC PC phosphatidylcholine PCA principal component analysis PE phosphatidylethanolamine QTOFMS quadrupole-time-of-flight mass spectrometry ROC receiving operator characteristic SAF steatosis activity and fibrosis SM T2DM type 2 diabetes mellitus TG triacylglycerol UHPLC ultrahigh-performance liquid chromatographyultrahigh-performance liquid chromatographyCELPC3-HBNAFLnon-alcoholic fatty liverTGtriacylglycerolNRR non-rejection rateLife Sciences & BiomedicineNAFLD non-alcoholic fatty liver diseaseFLIP Fatty Liver Inhibition of Progressionalanine aminotransferasemetaboliteCer ceramideCE cholesterol estersphingomyelinlysophosphatidylcholineand fibrosisALT alanine aminotransferaseInternal MedicineceramideNational Institute of Digestive Diseases and Kidney NASH Clinical Research NetworkAST3-HB 3-hydroxybutanoic acidQTOFMS quadrupole-time-of-flight mass spectrometryPCA principal component analysisLPC lysophosphatidylcholineHepatologynon-alcoholic fatty liver diseaseand clinical variablePC(O) ether PC3-hydroxybutanoic acidFibrosisNASH activity scoreNIDDK NASH-CRNlipid clusterlipid and metabolitephosphatidylethanolamineLipidomicsLMC lipid metabolite and clinical variableFFAHCC hepatocellular carcinomaJHEP reports : innovation in hepatology
researchProduct

Rapid antibiotic susceptibility testing on blood cultures using MALDI-TOF MS

2018

International audience; Antibiotic resistance is a major public health problem requiring the early optimization of antibiotic prescriptions. Matrix-Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometry (MALDI-TOF MS) has been shown to accurately identify bacteria from positive blood culture. Here, we developed a rapid detection of Escherichia coli resistance to amoxicillin (AMX) and cefotaxime (CTX) from positive blood culture based on MALDI-TOF MS. Potential sparing of broad-spectrum antibiotics was also evaluated. We tested 103 E. coli-positive blood cultures. Blood cultures were subculture 1-hour in antibiotic-free rich liquid media before further incubation with and with…

Agar Dilution Method0301 basic medicineTime FactorsCefotaximePhysiologyAntibioticslcsh:MedicineCefotaximeDrug resistancemedicine.disease_causeMass SpectrometryAnalytical ChemistrySpectrum Analysis TechniquesAntibioticsMicrobial PhysiologyMedicine and Health SciencesBlood cultureMatrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometrylcsh:ScienceMultidisciplinarymedicine.diagnostic_testbiologyAntimicrobialsChemistryMicrobial Growth and DevelopmentDrugsMatrix-Assisted Laser Desorption Ionization Mass SpectrometryBody FluidsAnti-Bacterial Agents3. Good healthChemistryBlood[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyPhysical SciencesAnatomyResearch Articlemedicine.drugmedicine.drug_class030106 microbiologyMicrobial Sensitivity TestsResearch and Analysis MethodsMicrobiologyAntibiotic Susceptibility TestingMicrobiology03 medical and health sciencesMicrobial ControlDrug Resistance BacterialEscherichia colimedicineHumansEscherichia coliPharmacologyBacteriological TechniquesBacterial Growthlcsh:RBiology and Life SciencesAmoxicillinAmoxicillinbiology.organism_classificationPharmacologic AnalysisBlood CultureAntibiotic ResistanceSpectrometry Mass Matrix-Assisted Laser Desorption-Ionizationlcsh:QAntimicrobial ResistanceSubculture (biology)BacteriaDevelopmental BiologyPLOS ONE
researchProduct

Validation of a novel automatic deposition of bacteria and yeasts on MALDI target for MALDI-TOF MS-based identification using MALDI Colonyst robot

2017

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) -based identification of bacteria and fungi significantly changed the diagnostic process in clinical microbiology. We describe here a novel technique for bacterial and yeast deposition on MALDI target using an automated workflow resulting in an increase of the microbes' score of MALDI identification. We also provide a comparison of four different sample preparation methods. In the first step of the study, 100 Gram-negative bacteria, 100 Gram-positive bacteria, 20 anaerobic bacteria and 20 yeasts were spotted on the MALDI target using manual deposition, semi-extraction, wet deposition onto 70% formic …

Atmospheric ScienceCarboxylic Acidslcsh:MedicineAnaerobic BacteriaResearch and Analysis MethodsMicrobiologyMass SpectrometryAnalytical ChemistryAutomationSpectrum Analysis TechniquesYeastsEnvironmental Chemistrylcsh:ScienceMatrix-Assisted Laser Desorption Ionization Time-of-Flight Mass SpectrometryGram Negative BacteriaBacteriaOrganic Compoundslcsh:REcology and Environmental SciencesGram Positive BacteriaFormic AcidOrganic ChemistryOrganismsFungiChemical CompoundsBiology and Life SciencesEukaryotaBacteriologyRoboticsYeastChemistryAtmospheric ChemistrySpectrometry Mass Matrix-Assisted Laser Desorption-IonizationPhysical SciencesEarth Scienceslcsh:QAcid DepositionAcidsResearch ArticlePLoS ONE
researchProduct

Rapid and Sensitive Identification of Major Histocompatibility Complex Class I-associated Tumor Peptides by Nano-LC MALDI MS/MS

2005

Identification of major histocompatibility complex (MHC)-associated peptides recognized by T-lymphocytes is a crucial prerequisite for the detection and manipulation of specific immune responses in cancer, viral infections, and autoimmune diseases. Unfortunately immunogenic peptides are less abundant species present in highly complex mixtures of MHC-extracted material. Most peptide identification strategies use microcapillary LC coupled to nano-ESI MS/MS in a challenging on-line approach. Alternatively MALDI PSD analysis has been applied for this purpose. We report here on the first off-line combination of nanoscale (nano) LC and MALDI TOF/TOF MS/MS for the identification of naturally proce…

CellsCellPeptideHuman leukocyte antigenMajor histocompatibility complexSensitivity and SpecificityBiochemistryEpitopeAnalytical ChemistryMajor Histocompatibility ComplexImmune systemCell Line TumormedicineHumansAmino Acid SequenceCarcinoma Renal CellMolecular Biologychemistry.chemical_classificationbiologyHistocompatibility Antigens Class IFlow CytometryMolecular biologyKidney NeoplasmsPeptide FragmentsNeoplasm Proteinsmedicine.anatomical_structurechemistrySpectrometry Mass Matrix-Assisted Laser Desorption-Ionizationbiology.proteinTime-of-flight mass spectrometryAntibodyMolecular & Cellular Proteomics
researchProduct

Properties and performance of a quadrupole mass filter used for resonance ionization mass spectrometry

1998

Abstract The performance of commercial quadrupole mass spectrometers (QMS) with a number of imperfections, as compared to the ideal hyperbolic geometry, has been characterized using the computer simulation program simion 3d version 6.0. The analysis of simulated QMS geometries focuses primarily on modeling of the internal potential, the study of field deviations, and the influence of finite length on performance of the QMS. The computer simulation of ion trajectories in the QMS field yields predictions for optimum working conditions and provides estimates for the resolving power and the maximum isotopic abundance sensitivity. Experimental measurements that confirm these expectations are pre…

ChemistrySelected reaction monitoringAnalytical chemistryThermal ionization mass spectrometryCondensed Matter PhysicsMass spectrometryTriple quadrupole mass spectrometerComputational physicsSelected ion monitoringPhysical and Theoretical ChemistryTime-of-flight mass spectrometryInstrumentationQuadrupole mass analyzerSpectroscopyHybrid mass spectrometerInternational Journal of Mass Spectrometry
researchProduct

Automatic Calibration of an Industrial RGB-D Camera Network Using Retroreflective Fiducial Markers

2019

This paper describes a non-invasive, automatic, and robust method for calibrating a scalable RGB-D sensor network based on retroreflective ArUco markers and the iterative closest point (ICP) scheme. We demonstrate the system by calibrating a sensor network comprised of six sensor nodes positioned in a relatively large industrial robot cell with an approximate size of 10 m × 10 m × 4 m . Here, the automatic calibration achieved an average Euclidean error of 3 c m at distances up to 9.45 m . To achieve robustness, we apply several innovative techniques: Firstly, we mitigate the ambiguity problem that occurs when detecting a marker at long range or low resolution by comparing the…

Computer scienceComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION02 engineering and technologylcsh:Chemical technologytime-of-flightBiochemistryArticleVDP::Food science and technology: 600Analytical Chemistrylaw.inventionIndustrial robotlawRegion of interestRobustness (computer science)automatic calibration0202 electrical engineering electronic engineering information engineeringCalibrationVDP::Næringsmiddelteknologi: 600lcsh:TP1-1185Computer visionElectrical and Electronic EngineeringInstrumentationbusiness.industryambiguity problemIterative closest point3D sensors020207 software engineeringretroreflective markersAtomic and Molecular Physics and OpticsTime of flightTheoryofComputation_MATHEMATICALLOGICANDFORMALLANGUAGESRGB color model020201 artificial intelligence & image processingArtificial intelligencebusinessFiducial markerWireless sensor networkSensors
researchProduct

Embedded Processing and Compression of 3D Sensor Data for Large Scale Industrial Environments

2019

This paper presents a scalable embedded solution for processing and transferring 3D point cloud data. Sensors based on the time-of-flight principle generate data which are processed on a local embedded computer and compressed using an octree-based scheme. The compressed data is transferred to a central node where the individual point clouds from several nodes are decompressed and filtered based on a novel method for generating intensity values for sensors which do not natively produce such a value. The paper presents experimental results from a relatively large industrial robot cell with an approximate size of 10 m &times

Computer sciencePoint cloud02 engineering and technologylcsh:Chemical technologytime-of-flightBiochemistryArticleAnalytical ChemistryComputational sciencelaw.inventionIndustrial robotOctreelawpoint clouds0202 electrical engineering electronic engineering information engineeringdenoisinglcsh:TP1-1185Electrical and Electronic EngineeringInstrumentationlidarscalabilityLocal area network020206 networking & telecommunications020207 software engineering3D sensorscompressionAtomic and Molecular Physics and OpticsScalabilitySensors (Basel, Switzerland)
researchProduct