Search results for "Uber"

Global Distribution and Evolution of Mycobacterium bovis Lineages

2020

Mycobacterium bovis is the main causative agent of zoonotic tuberculosis in humans and frequently devastates livestock and wildlife worldwide. Previous studies suggested the existence of genetic groups of M. bovis strains based on limited DNA markers (a.k.a. clonal complexes), and the evolution and ecology of this pathogen has been only marginally explored at the global level. We have screened over 2,600 publicly available M. bovis genomes and newly sequenced four wildlife M. bovis strains, gathering 1,969 genomes from 23 countries and at least 24 host species, including humans, to complete a phylogenomic analyses. We propose the existence of four distinct global lineages of M. bovis (Lb1, …

IP-10 is an accurate biomarker for the diagnosis of tuberculosis in children.

2014

Summary Objective Performance of IFN-γ assays in children is compromised. Therefore, we investigated the utility of IP-10 for the detection of active tuberculosis (TB) and latent tuberculosis infection (LTBI) diagnosis in children; comparing its positivity with QuantiFERON-TB Gold In-Tube (QFN-G-IT) and T-SPOT.TB. Methods We studied 230 children from three groups: active TB, screening (healthy children without known exposure to active TB patient screened at school or by their paediatrician) and contact-tracing studies. IFN-γ release was determined by QFN-G-IT and T-SPOT.TB. IP-10 was detected in QFN-G-IT supernatants by ELISA. Results When combining QFN-G-IT and IP-10 assays, positive resul…

ESAT-6 Peptide Recognition by Bovine CD8 + Lymphocytes of Naturally Infected Cows in Herds from Southern Italy

2006

ABSTRACT The aim of this study was to define epitopes of Mycobacterium bovis from ESAT-6 (early secretory antigen of 6 kDa) recognized by CD8 + T lymphocytes from cows naturally infected with Mycobacterium bovis . We found that bovine CD8 + T cells recognized 10 out of 11 ESAT-6 peptides tested.

Inducing properties of rifampicin and rifabutin for selected enzyme activities of the cytochrome P-450 and UDP-glucuronosyltransferase superfamilies …

1996

Important species differences have been reported concerning the induction properties of rifampicin towards enzymes of the P-450 superfamily. Mice, rabbits and humans are far more responsive than rats and guinea pigs. In the present study a strong induction of cytochrome P-450 3A-dependent enzyme activities was observed in female rat liver microsomes after high dose treatment (> or = 250 mg/kg/day for 9 days) with rifampicin, resulting in an up to 30-fold enhanced hydroxylation rate of testosterone in the 2 beta-, 6 beta- and 15 beta-position in vitro. Other cytochrome P-450 isozyme-selective reactions were not, or only marginally, affected. A steep increase in cytochrome P-450 3A activity o…

Commemorating World TB Day 2020: “IT’S TIME” — It’s time to End the Global TB Epidemic

2020

Time course of mycobacterial infection of dendritic cells in the lungs of intranasally infected mice

2004

Summary Setting : Dendritic cells (DC) could regulate between the protective and pathogenic immune responses following tuberculous infection. In this paper we investigated if their early infection in the lungs represents a plausible alternative to cross-priming with mycobacterial antigens acquired from infected macrophages. Objective : To determine the extent and time course of infection of lung DCs following intranasal inoculation of BALB/c mice with green fluorescent protein (GFP) tagged Bacillus Calmette-Guerin (BCG). Results : A fraction of GFP-BCG infected lung cells were classified as monocytic DCs with the CD11c + IA + 33D1 + CD8a − phenotype. These cells represented 5–18% of the tot…

Anti-16-kilodalton mycobacterial protein immunoglobulin M levels in healthy but purified protein derivative-reactive children decrease after chemopro…

2007

ABSTRACT Serum responses against Mycobacterium tuberculosis HSP16 were determined for children with tuberculosis (TB) and for healthy purified protein derivative (PPD)-positive and PPD-negative children. Immunoglobulin G (IgG) and IgM responses were higher for TB patients than for other groups. After chemotherapy, IgM and IgG responses decreased for TB patients and PPD-positive subjects. Monitoring of anti- M. tuberculosis HSP16 responses could assist in the management of pediatric TB.

Die synergistische Wirkung von INH und 6-(Sulfanilamido)-2,4-dimethylpyrimidin gegen resistente Tuberkelbakterienstämme (in vitro-Versuche)

1954

INH (Rimifon) und 6-(Sulfanilamido)-2,4-Dimethylpyrimidin (Aristamid) haben eine synergistische Wirkung gegen sensible und gegen streptomycin- und INH-resistente Tuberkelbakterien-Stamme.

Whole-genome sequencing of Mycobacterium tuberculosis directly from clinical samples for high-resolution genomic epidemiology and drug resistance sur…

2020

9 páginas, 3 figuras

Der Mechanismus der Tuberkulincytolyse

1964

Weise Blutzellen oder Peritonealzellen Tb-sensibilisierter Meerschweinchen losen sich in Abwesenheit von Serum nach Alttuberkulinzugabe zu einem hohen Prozentsatz auf. Das gereinigte Tuberkulin „GT“ zeigt diesen Effekt nur in Gegenwart von Serum-Komplement. Die Cytolyse-Reaktion mit GT in Verbindung mit Komplement tritt nach einer Sensibilisierung mit BCG fruher auf als die Tuberkulin-Hautreaktion mit GT. Peritonealzellen von nur mit Alttuberkulin sensibilisierten Tieren zeigen ebenfalls das Phanomen der Alttuberkulin-Cytolyse. Verdunnte Seren von Tuberkulin-sensibilisierten Tieren, die nach den Reaktionen vonMiddlebrook u.Dubos keinen Antikorpertiter besasen, bewirkten mit Alttuberkulin ei…