Search results for "VALUATION"
showing 10 items of 2494 documents
Antitumor effects of dehydroxymethylepoxyquinomicin, a novel nuclear factor-kappaB inhibitor, in human liver cancer cells are mediated through a reac…
2009
Activation of the nuclear transcription factor-kappa B (NF-kappa B) has been implicated in liver tumorigenesis. We evaluated the effects of a novel NF-kappa B inhibitor, dehydroxymethylepoxyquinomicin (DHMEQ), in two human liver cancer cell lines HA22T/VGH and HuH-6. DHMEQ treatment dose dependently decreased the DNA-binding capacity of the NF-kappa B p65 subunit, inhibited cell growth and proliferation, and increased apoptosis as shown by caspase activation, release of cytochrome c, poly(ADP-ribose) polymerase cleavage, and down-regulation of survivin. DHMEQ also induced a dose-dependent activation of mitogen-activated protein kinase kinase/extracellular signal-regulated kinase signaling, …
Early stage diagnosis of programmed cell death (apoptosis) using electroanalysis: Nanomaterial and methods overview
2017
Timely monitoring of apoptosis can effectively assist early diagnosis of related diseases and continuous evaluation of the efficacy of drugs. Therefore, developing advanced methods for apoptosis evaluation has attracted more and more attention. In addition, the increased demand for understanding the early stages of apoptosis is pushing the envelope for solutions in early instance real-time monitoring of death kinetics. In this review, various types of electro-analytical methods for detection of apoptosis were discussed. The emphasis is placed in diagnosis of apoptosis based on cyto-, immune-, geno-, apta-, and peptide based biosensing strategies. In the first part of review, various electro…
L'écriture des documents numériques : vers une cyber-rhétorique
2008
Laboratoire GRESEC - axe CRISTAL ICM, Université Stendhal Grenoble III; National audience; Cet ouvrage traite de la conception de documents numériques utilisés en contexte professionnel et étudie en particulier les deux thèmes suivants : le découpage de l'information pour la mise en écran, les organisateurs de structure, de navigation et de mise en relief de l'information. L'influence sur la lecture des dispositifs de mise en forme matérielle et des organisateurs de structure et de navigation est souvent méconnue. Aussi les méthodes destinées à tester le rôle des dispositifs de mise en forme revêtent-elles une importance capitale et sont présentées à la fin de cet ouvrage. Les spécificités …
La documentation à l'heure du numérique : répercussions sur les usages des publics et sur le rôle des documentalistes
2008
Journée d'études co-organiée avec la librairie Lavoisier et l'ADBS Rhône-Alpes Lyon. Evolution de l'offre éditoriale numérique française, répercussions sur les usages des publics, impact sur le rôle des documentalistes. ENS Lettres; National audience; Évolution de l'offre documentaire ; formation ; alphabétisation technologique en contexte professionnel
Backing up the development of teaching skills of college teachers with an original conceptual frame
2010
National audience; L'objectif de cet article est de présenter un cadre conceptuel issu d'une expertise collective, développée pendant trois années au sein d'un projet international. Ce cadre permet de formaliser le conseil et l'accompagnement pédagogique au niveau universitaire, en questionnant les défis et enjeux de la pratique du développement pédagogique vis-à-vis des enseignants, des équipes d'enseignants et des responsables des institutions universitaires. Il permet également de soulever un ensemble de pistes de recherches qui restent à développer autour de la thématique du soutien à l'enseignement universitaire.
Getting Docking into Shape Using Negative Image-Based Rescoring
2019
The failure of default scoring functions to ensure virtual screening enrichment is a persistent problem for the molecular docking algorithms used in the structure-based drug discovery. To remedy this problem, elaborate rescoring and post-processing schemes have been developed with a varying degree of success, specificity, and cost. The negative imagebased rescoring (R-NiB) has been shown to improve the flexible docking performance markedly with a variety of drug targets.The yield improvement is achieved by comparing the alternative docking poses against the negative image of the target protein’s ligand-binding cavity. In other words, the shape and electrostatics of the binding pocket is dir…
Targeting SARS-CoV-2 RBD Interface: a Supervised Computational Data-Driven Approach to Identify Potential Modulators
2020
Coronavirus disease 2019 (COVID-19) has spread out as a pandemic threat affecting over 2 million people. The infectious process initiates via binding of SARS-CoV-2 Spike (S) glycoprotein to host angiotensin-converting enzyme 2 (ACE2). The interaction is mediated by the receptor-binding domain (RBD) of S glycoprotein, promoting host receptor recognition and binding to ACE2 peptidase domain (PD), thus representing a promising target for therapeutic intervention. Herein, we present a computational study aimed at identifying small molecules potentially able to target RBD. Although targeting PPI remains a challenge in drug discovery, our investigation highlights that interaction between SARS-CoV…
Bioassays to monitor taspase1 function for the identification of pharmacogenetic inhibitors
2011
Background Threonine Aspartase 1 (Taspase1) mediates cleavage of the mixed lineage leukemia (MLL) protein and leukemia provoking MLL-fusions. In contrast to other proteases, the understanding of Taspase1's (patho)biological relevance and function is limited, since neither small molecule inhibitors nor cell based functional assays for Taspase1 are currently available. Methodology/Findings Efficient cell-based assays to probe Taspase1 function in vivo are presented here. These are composed of glutathione S-transferase, autofluorescent protein variants, Taspase1 cleavage sites and rational combinations of nuclear import and export signals. The biosensors localize predominantly to the cytoplasm…
Review of current and “omics” methods for assessing the toxicity (genotoxicity, teratogenicity and nephrotoxicity) of herbal medicines and mushrooms
2012
Ethnopharmacological relevance: The increasing use of traditional herbal medicines around the world requires more scientific evidence for their putative harmlessness. To this end, a plethora of methods exist, more or less satisfying. In this post-genome era, recent reviews are however scarce, not only on the use of new "omics" methods (transcriptomics, proteomics, metabonomics) for genotoxicity, teratogenicity, and nephrotoxicity assessment, but also on conventional ones. Methods: The present work aims (i) to review conventional methods used to assess genotoxicity, teratogenicity and nephrotoxicity of medicinal plants and mushrooms; (ii) to report recent progress in the use of "omics" techn…
The first milestone in psychoeducational intervention in Spain: the Medical and Educational Psychology Laboratory of the National Patronage for the H…
2017
espanolLa intervencion psicopedagogica terapeutica supone el inicio de una importante vertiente del trabajo psicotecnico en Espana, destacando especialmente la atencion al ambito infantil, que propiciara la creacion de instituciones como el Patronato Nacional de Anormales, que supondra un referente para el trabajo conjunto de profesionales del ambito medico, pedagogico y psicologico. Este trabajo analiza los primeros anos del Patronato, centrandose en sus objetivos e ideario de funcionamiento y, especialmente en la creacion en 1914 del Laboratorio Medico y de Psicologia Pedagogica y en el trabajo que alli realizaron autores proximos a la psiquiatria infantil como Rodriguez Lafora y Achucarr…