Search results for "Valuation"

Luminometric sub-nanoliter droplet-to-droplet array (LUMDA) and its application to drug screening by phase I metabolism enzymes.

2012

Here we show the fabrication of the Luminometric Sub-nanoliter Droplet-to-droplet Array (LUMDA chip) by inkjet printing. The chip is easy to be implemented and allows for a multiplexed multi-step biochemical assay in sub-nanoliter liquid spots. This concept is here applied to the integral membrane enzyme CYP3A4, i.e. the most relevant enzymatic target for phase I drug metabolism, and to some structurally-related inhibitors.

Determination of Plasma Lipid Hydroperoxides by an NADPH/NADP + Coupled Enzyme Reaction System. Evaluation of a Method

1998

Summary: Several techniques based on different principles have been proposed to measure lipid hydroperoxides. Enzymatic methods are sensitive and can be quite specific but they are subject to interference by inhibitors and not all are stoichiometric. The present work proposes some modifications of the Heath & Tappel (Anal Biochem 1976; 7:184—91) enzymatic method of determination of lipid hydroperoxides in order to standardize and automate it and to meet the analytical criteria required for a biological assay. The proposed new protocol and the automated assay give acceptable within-run and between-run precisions, with coefficients of variation of 3.34% and 5.80%, respectively, at the usual p…

Design, synthesis, biological evaluation and molecular modelling of substituted pyrrolo[2,1-a]isoquinolinone derivatives: discovery of potent inhibit…

2021

We report here the design, synthesis and biological evaluation of a new series of substituted pyrrolo[2,1-a]isoquinolin-3-one derivatives, some of which have strong inhibitory activity against both AChE and BChE enzymes. The design of these new inhibitors was carried out taking rivastigmine as the starting structure. Thus, on the basis of an exhausting molecular modeling study using combined techniques (docking, dynamic molecular simulations and QTAIM calculations), we obtained new ligands possessing stronger inhibitory effects than rivastigmine, the reference compound. QTAIM analysis gave us detailed information about the molecular interactions stabilizing the different ligand–enzyme compl…

Determination of fatty acids and lipid classes in salmon oil by near infrared spectroscopy

2017

International audience; Near-infrared (NIR) spectroscopy was evaluated as a rapid method for the determination of oleic, palmitic, linoleic and linolenic acids as well as omega-3, omega-6, and to predict polyunsaturated, monounsaturated and saturated fatty acids, together with triacylglycerides, diglycerides, free fatty acids and ergosterol in salmon oil. To do it, Partial Least Squares (PLS) regression models were applied to correlate NIR spectra with aforementioned fatty acids and lipid classes. Results obtained were validated in front of reference procedures based on high performance thin layer and gas chromatography. PLS-NIR has a good predictive capability with relative root mean squar…

État actuel des réseaux de mesures éco-climatiques en Afrique centrale : les ambitions du projet de recherche international FORGREENE.

2016

This work presents an overview of spatio-temporal quality andspatial density of rainfall networks of Cameroon, Gabon, Congo, CAP and DRC, particularly in the recentperiod (1973-2014) through the GSOD international database. Faced with enormous gaps observed, theseresults will help to select metrological and analytical orientations in FORGREENE project (Tropical forestsgreeness and cloudiness in Central Africa: present conditions and future evolution). A comparison with rainfallsatellite estimates (RFE type) also indicates their great potential, provided they are based on in situ validations.

Conducting sensory analysis tests among the elderly

2015

A cognitive/decision-making model of evaluation for ethical urban and building renewal

2009

Creativity is a strategic factor not only in the production sector, but also for planning, development and management of the city. The physical shape of the city is determined by the plan of buildings and settlements as a whole. Nevertheless, the functional and aesthetical criteria alone are inadequate, if you adopt the view of sustainability. In particular, from biological, ecological and economic points of view, the shape of the sustainable city is the result of a process of creative choice of traditional and innovative forms, materials and technologies that contribute to the quality of buildings and urban ambient. This principle applies a fortiori in cities interested by not-negligible p…

Miejsce i znaczenie oceny jakości informacji w wybranych modelach ewaluacji bibliotek cyfrowych

2016

Teza/cel artykułu - Zapewnienie odpowiedniej jakości zasobów informacji w bibliotekach cyfrowych stanowi nowe wyzwanie dla dalszego intensywnego rozwoju tego rodzaju wirtualnych organizacji. Celem artykułu jest ukazanie miejsca i znaczenia oceny jakości informacji w najważniejszych modelach ewaluacji bibliotek cyfrowych. Metody - Jako metodę badawczą zastosowano analizę porównawczą wybranych modeli bibliotek cyfrowych pod względem sposobu ujęcia oceny jakości informacji w strukturze modelu, zakresu tej oceny oraz proponowanych metod i technik oceny. Wyniki/ wnioski - W badanych modelach przedmiotem oceny były zarówno dane, jak i metadane. Przeprowadzona analiza wykazała także, że rozważane …

Les théoriciens spontanés : comment les évaluateurs construisent et révisent leurs connaissances des programmes par l'expérience

2021

International audience; What exactly do evaluators learn about programs through experience and how? We chose a constructivist framework to investigate the structure of evaluators’ program-related knowledge, namely the form, content, and origins of their expressed theories. In this context, we complemented a Piagetian theory of learning with new developments offered by “probabilistic” (or “Bayesian”) models of learning. We conducted “explicitation” interviews with nine experienced practitioners, some specializing in the education or agri-environment sectors and some generalists. After examining the form and content of their program-related knowledge, we examine its development through experi…

Dagli obiettivi educativi alle competenze fondamentali

2014

Insegnare e valutare per competenze costituisce la "nuova frontiera" della Didattica contemporanea. Il libro si colloca nella prospettiva della scuola delle competenze senza però disperdere quanto di positivo è stato acquisito nella pratica della didattica per obiettivi di "terza generazione", quella che fa riferimento al sistema degli obiettivi educativi "fondamentali". Si ritiene che le critiche alla progettazione e alla valutazione per obiettivi abbiano contribuito a diffondere una concezione più ampia degli obiettivi educativi, che ben si integra con la nuova prospettiva didattica delle competenze. Il sistema degli obiettivi fondamentali dell'educazione, descritto nel volume, costituisc…