Search results for "XX"
showing 10 items of 460 documents
The Greenland shark Somniosus microcephalus—Hemoglobins and ligand-binding properties
2017
A large amount of data is currently available on the adaptive mechanisms of polar bony fish hemoglobins, but structural information on those of cartilaginous species is scarce. This study presents the first characterisation of the hemoglobin system of one of the longest-living vertebrate species (392 +/- 120 years), the Arctic shark Somniosus microcephalus. Three major hemoglobins are found in its red blood cells and are made of two copies of the same a globin combined with two copies of three very similar beta subunits. The three hemoglobins show very similar oxygenation and carbonylation properties, which are unaffected by urea, a very important compound in marine elasmobranch physiology.…
Making sense of big data in health research: {T}owards an {EU} action plan
2016
Genome medicine 8(1), 71 (2016). doi:10.1186/s13073-016-0323-y
Transmembrane signaling and cytoplasmic signal conversion by dimeric transmembrane helix 2 and a linker domain of the DcuS sensor kinase
2020
Transmembrane (TM) signaling is a key process of membrane-bound sensor kinases. The C4-dicarboxylate (fumarate) responsive sensor kinase DcuS of Escherichia coli is anchored by TM helices TM1 and TM2 in the membrane. Signal transmission across the membrane relies on the piston-type movement of the periplasmic part of TM2. To define the role of TM2 in TM signaling, we use oxidative Cys cross-linking to demonstrate that TM2 extends over the full distance of the membrane and forms a stable TM homodimer in both the inactive and fumarate-activated state of DcuS. An S186xxxGxxxG194 motif is required for the stability and function of the TM2 homodimer. The TM2 helix further extends on the periplas…
Evidence-Based Clinical Use of Nanoscale Extracellular Vesicles in Nanomedicine
2016
collaboration au projet H2020 European Cooperation in Science and Technology (COST) program European Network on Microvesicles and Exosomes in Health and Disease (ME-HAD); International audience; Recent research has demonstrated that all body fluids assessed contain substantial amounts of vesicles that range in size from 30 to 1000 nm and that are surrounded by phospholipid membranes containing different membrane microdomains such as lipid rafts and caveolae. The most prominent representatives of these so-called extracellular vesicles (EVs) are nanosized exosomes (70-150 nm), which are derivatives of the endosomal system, and microvesicles (100-1000 nm), which are produced by outward budding…
Characterization of sulfhydryl oxidase from Aspergillus tubingensis
2017
Background Despite of the presence of sulfhydryl oxidases (SOXs) in the secretomes of industrially relevant organisms and their many potential applications, only few of these enzymes have been biochemically characterized. In addition, basic functions of most of the SOX enzymes reported so far are not fully understood. In particular, the physiological role of secreted fungal SOXs is unclear. Results The recently identified SOX from Aspergillus tubingensis (AtSOX) was produced, purified and characterized in the present work. AtSOX had a pH optimum of 6.5, and showed a good pH stability retaining more than 80% of the initial activity in a pH range 4-8.5 within 20 h. More than 70% of the initia…
An Illustration to Ælfric’s De temporibus anni in Ælfwine’s Prayerbook
2020
L'articolo esamina un diagramma contenuto nel manoscritto London, British Library, Cotton Titus D.xxvii+xxvi, generalmente noto come 'Ælfwine’s Prayerbook'. Il diagramma, che si trova al fol. 21 v , è stato in genere interpretato come una rota incompleta, o delle maree o dei venti (dal momento che contiene solo 4 dei canonici 12 venti della tradizione scientifica medievale). Una analisi comparativa e dettagliata delle caratteristiche del diagramma, tuttavia, mostra come l'ipotesi della rota delle maree sia da rigettare in favore della teoria di una rota ventorum. Inoltre, l'analisi dei testi contenuti nel manoscritto rivela una stretta connessione tra il diagramma e il De temporibus anni di…
Le Dictionnaire de l’Académie française source des dictionnaires bilingues franco-italiens de 1871 à nos jours
2017
L'articolo si propone di sondare l'influenza del Dictionnaire de l'Académie française sulla lessicografia bilingue franco-italiana in un arco cronologico compreso tra il 1871 e il 2000. In particolare, si passano in rassegna le prefazioni dei principali dizionari bilingui pubblicati nel periodo considerato, al fine di valutare se il Dictionnaire de l'Académie venga citato tra le fonti e qual uso il lessicografo intenda farne. The article aims to explore the influence of the Dictionnaire de l'Académie française on French-Italian bilingual lexicography in a chronological arc between 1871 and 2000. In particular, the prefaces of the main bilingual dictionaries published in the period considere…
Architectures en vertige et « topographie » du personnage chez Julien Green
2018
L’architettura è senza dubbio uno degli assi fondamentali attorno al quale si dispiega l’immaginario di Julien Green, la cui opera è ricca di dimore, castelli, camere, biblioteche, spazi urbani. Attraverso l’analisi di tre romanzi (Mont-Cinère, Adrienne Mesurat, Minuit), l’articolo si propone di mostrare, per mezzo del riferimento metodologico ai Séminaires e agli Écrits di Lacan, che gli spazi architettonici rappresentati assumono nello scrittore una dimensione trascendente che realizza lo slittamento dagli spazi reali alla topologia soggettiva dei personaggi. Architecture is undeniably one of the fundamental axes around which the imagery of Julien Green unfolds, whose work is full of hous…
Les tours dits de la chair dans les autofictions de Philippe Vilain
2016
Le autofinzioni di Philippe Vilain si distinguono per una meticolosa ricerca stilistica e una pratica di riscrittura supposta mettere a nudo la pelle dello scrittore. La scrittura della carne è particolarmente influenzata da tale ricerca: ciò che la sostiene non è tanto ciò cui lo specchio scritturale ci rimanda, bensì ciò che è nascosto nell'implicito dei ricordi in cui i pezzi del godimento si embricano in pezzi di linguaggio. Questo articolo si propone di analizzare la posta in gioco della scrittura autofinzionale di Philippe Vilain nei suoi principali romanzi attraverso la topologia psicoanalitica di Lacan. Philippe Vilain's autofictions are distinguished by a meticulous stylistic resea…
Small Residues Inhibit Homo-Dimerization of the Human Carbonic Anhydrase XII Transmembrane Domain
2021
Amino acids with small side chains and motifs of small residues in a distance of four are rather abundant in human single-span transmembrane helices. While interaction of such helices appears to be common, the role of the small residues in mediating and/or stabilizing transmembrane helix oligomers remains mostly elusive. Yet, the mere existence of (small)xxx(small) motifs in transmembrane helices is frequently used to model dimeric TM helix structures. The single transmembrane helix of the human carbonic anhydrases XII contains a large number of amino acids with small side chains, and critical involvement of these small amino acids in dimerization of the transmembrane domain has been sugges…