Search results for "acids"

showing 10 items of 3520 documents

Inhibition of γ-glutamyl transpeptidase decreases amino acid uptake in human keratinocytes in culture

1990

Abstract Acivicin inhibits γ-glutamyl transpeptidase activity in human keratinocytes in culture. Treatment of these cells with acivicin produces a decrease in the uptake of L-[U-14C]alanine, 2-amino-[1-14C]-isobutyrate, L[U-14C]leucine and l-aminocyclopentane-l-[14C]carboxylate. D-[U-14C]glucose uptake is not affected by the presence of acivicin. These results support, for the first time in vitro, the hypothesis that the γ-glutarml cycle may be involved in amino acid uptake by human cells.

KeratinocytesGlucose uptakeAmino acid transportHuman keratinocyteBiophysicsIn Vitro TechniquesBiologyBiochemistrychemistry.chemical_compoundStructural BiologyGeneticsHumansAmino AcidsMolecular BiologyAcivicinCells Culturedchemistry.chemical_classificationAlanineBiological TransportIsoxazolesgamma-GlutamyltransferaseCell BiologyGlutathioneGlutathioneIn vitroγ-Glutamyl transpeptidaseGlucoseEnzymechemistryBiochemistryCell cultureCell cultureLeucineFEBS Letters
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Unsaturated Fatty Acids Drive Disintegrin and Metalloproteinase (ADAM)-dependent Cell Adhesion, Proliferation, and Migration by Modulating Membrane F…

2011

The disintegrin-metalloproteinases ADAM10 and ADAM17 mediate the release of several cell signaling molecules and cell adhesion molecules such as vascular endothelial cadherin or L-selectin affecting endothelial permeability and leukocyte transmigration. Dysregulation of ADAM activity may contribute to the pathogenesis of vascular diseases, but the mechanisms underlying the control of ADAM functions are still incompletely understood. Atherosclerosis is characterized by lipid plaque formation and local accumulation of unsaturated free fatty acids (FFA). Here, we show that unsaturated FFA increase ADAM-mediated substrate cleavage. We demonstrate that these alterations are not due to genuine ch…

KeratinocytesMembrane FluidityADAM10Lipid BilayersVascular permeabilityBiologyADAM17 ProteinBiochemistryCapillary PermeabilityADAM10 ProteinCell MovementMembrane fluidityCell AdhesionAnimalsHumansCell adhesionMolecular BiologyCell ProliferationCell adhesion moleculeCell growthFluorescence recovery after photobleachingEndothelial CellsMembrane ProteinsCell BiologyAtherosclerosisADAM ProteinsCell biologyLipoproteins LDLADAM ProteinsHEK293 CellsFatty Acids UnsaturatedCholesterol EstersRabbitsAmyloid Precursor Protein SecretasesGranulocytes
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Synthesis and biological evaluation of dehydroabietic acid derivatives.

2010

A series of C18-oxygenated derivatives of dehydroabietic acid were synthesized from commercial abietic acid and evaluated for their cytotoxic, antimycotic, and antiviral activities.

KetoneAntifungal AgentsCarboxylic acidAntineoplastic AgentsHerpesvirus 1 HumanMicrobial Sensitivity TestsAldehydeChemical synthesisAntiviral Agentschemistry.chemical_compoundInhibitory Concentration 50Cell Line TumorDrug DiscoveryOrganic chemistryAnimalsHumansAbietic acidCytotoxicityPharmacologychemistry.chemical_classificationOrganic ChemistryFungifood and beveragesGeneral Medicineequipment and suppliesTerpenoidchemistryAbietaneslipids (amino acids peptides and proteins)DiterpeneHydrophobic and Hydrophilic InteractionsEuropean journal of medicinal chemistry
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Enantioselective addition of nitromethane to α-keto esters catalyzed by copper(ii)–iminopyridine complexes

2008

The copper complex of a chiral iminopyridine easily prepared from (R)-(-)-fenchone and picolylamine catalyzes the enantioselective Henry (nitroaldol) reaction between nitromethane and alpha-keto esters. Good yields and modest to good enantioselectivities are obtained for a wide range of alpha-keto esters, bearing aromatic, alkyl or alkenyl groups attached to the ketone carbonyl group.

KetonePyridineschemistry.chemical_elementLigandsBiochemistryMedicinal chemistryCatalysisNitroparaffinsSubstrate SpecificityCatalysischemistry.chemical_compoundOrganic chemistryPhysical and Theoretical ChemistryAlkylchemistry.chemical_classificationCopper complexNitromethaneChemistryOrganic ChemistryEnantioselective synthesisEstersStereoisomerismCopperCarbonyl grouplipids (amino acids peptides and proteins)MethaneCopperOrg. Biomol. Chem.
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HDL Subtractions Distribution in Renal Transplant Recipients: Lack of Evidence of a Reduction of HDL<sub>2</sub> Particles

1996

Since the high rate of cardiovascular disease in renal transplant recipients, alterations of lipoprotein profile in such patients were extensively evaluated, but the HDL subclass profile was not completely clarified. Renal transplant recipients usually show normal to high plasma levels of HDL cholesterol, even if some investigations suggested a persistence of low HDL2 levels: this was not useful in terms of cardiovascular protection. We designed this study in order to evaluate HDL subfractions distribution in renal transplant recipients. We studied 55 renal transplant recipients, treated with prednisone, azathioprine and/or cyclosporine, and 34 healthy normolipidemics as controls. In all su…

Kidneymedicine.medical_specialtyVascular diseasebusiness.industryCholesterolAzathioprinemedicine.diseaseTransplantationchemistry.chemical_compoundmedicine.anatomical_structureEndocrinologychemistryPrednisoneInternal medicinemedicinelipids (amino acids peptides and proteins)businessComplicationmedicine.drugLipoproteinNephron
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Initiatoren für die polymerisation des trioxane. 20. Mitt. über polyoxymethylene1

1963

Die Polymerisation des Trioxans bei 60°C in Cyclohexan mit verschiedenen kationischen Initiatoren wurde untersucht. Die aktivsten Initiatoren sind Acetylperchlorat, Perchlorsaure, FeCl3 und SnCl4. Die Molekulargewichte der erhaltenen Polyoxymethylene sind bei den aktivsten Initiatoren am hochsten. Der Einflus der ubertragung durch Wasser und der kinetischen Kettenlange auf die Molekulargewichte wird diskutiert. Wahrend der Polymerisation kann durch den Initiator eine Spaltung bereits gebildeter Makromolekule erfolgen, welche die Molekulargewichte stark reduziert. Die Frage der Cokatalyse bei der kationischen Polymerisation des Trioxans mit Ansolvosauren wurde untersucht. Bei der Polymerisat…

Kinetic chain lengthPerchloratechemistry.chemical_compoundchemistryPolymerizationCyclohexaneTrioxanePolymer chemistryCationic polymerizationLewis acids and basesPerchloric acidDie Makromolekulare Chemie
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Automated untargeted stable isotope assisted lipidomics of liver cells on high glucose shows alteration of sphingolipid kinetics

2020

Abstract Untargeted lipidomics is a powerful tool to discover new biomarkers and to understand the physiology and pathology of lipids. The use of stable isotopes as tracers to investigate the kinetics of lipids is another tool able to supply dynamic information on lipid synthesis and catabolism. Coupling the two methodology is then very appealing in the study of lipid metabolism. The main issue to face is to perform thousands of calculations in order to obtain kinetic parameters starting from the MS raw data. An automated computerized routine able to do accomplish such task is presented in this paper. We analyzed the lipid kinetics of palmitic acid (PA) in hepatoma liver cells cultured in v…

KineticsPalmitic AcidHep G2 CellFatty Acids NonesterifiedOrbitrapHigh resolution mass spectrometry01 natural sciencesGas Chromatography-Mass SpectrometryWorkflowlaw.inventionPalmitic acidAutomation03 medical and health scienceschemistry.chemical_compoundInsulin resistancelawLipidomicsmedicineHumansMolecular Biology030304 developmental biologyKineticSphingolipids0303 health sciencesChromatographyChemistryLipidomic010401 analytical chemistryInsulin resistanceLipid metabolismHep G2 CellsCell BiologyDeuteriumLipid Metabolismmedicine.diseaseCulture Media0104 chemical sciencesKineticsGlucoseIsotope LabelingLipidomicsCell modelHepatocytesMonoisotopic massSphingomyelinAlgorithmsSoftwareBiochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
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Structural differences of prebiotic oligosaccharides influence their capability to enhance iron absorption in deficient rats

2014

This study evaluates the influence of novel galacto-oligosaccharides derived from lactulose (GOS-Lu), kojibiose or 4′-galactosyl-kojibiose in hematological parameters of Fe homeostasis using Fe-deficient animals. Liver TfR-2, IL-6, NFκB and PPAR-γ expression (mRNA) were also determined by RT-qPCR analyses, and active hepcidin peptide production and short chain fatty acids by LC coupled to MS/MS or UV detection. Feeding animals with GOS-Lu or kojibiose together with FeCl3 increased hemoglobin (Hb) production (by 17%) and mean Hb concentration into erythrocytes relative to animals administered with FeCl3 alone (14.1% and 19.7%, respectively). Animals administered with prebiotics showed decrea…

Kojibiosemedicine.medical_treatmentPeptideAbsorption (skin)Ferric CompoundsIntestinal absorptionHemoglobinschemistry.chemical_compoundLactuloseChloridesHepcidinsTandem Mass SpectrometryHepcidinReceptors TransferrinmedicineAnimalsHomeostasisMicronutrientsRNA MessengerRats Wistarchemistry.chemical_classificationAnemia Iron-DeficiencybiologyInterleukin-6ChemistryPrebioticNF-kappa BGeneral MedicineFatty Acids VolatileRatsPPAR gammaDisease Models AnimalPrebioticsIntestinal AbsorptionLiverBiochemistryDietary Supplementsbiology.proteinFemaleTrisaccharidesIron DietaryHomeostasisFood Sciencemedicine.drugFood Funct.
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Suitability of Phytosterols Alongside Fatty Acids as Chemotaxonomic Biomarkers for Phytoplankton

2016

The composition and abundance of phytoplankton is an important factor defining ecological status of marine and freshwater ecosystems. Chemotaxonomic markers (e.g., pigments and fatty acids) are needed for monitoring changes in a phytoplankton community and to know the nutritional quality of seston for herbivorous zooplankton. Here we investigated the suitability of sterols along with fatty acids as chemotaxonomic markers using multivariate statistics, by analyzing the sterol and fatty acid composition of 10 different phytoplankton classes including altogether 37 strains isolated from freshwater lakes. We were able to detect a total of 47 fatty acids and 29 sterols in our phytoplankton sampl…

LAKES0106 biological sciencesDINOFLAGELLATEta1172ChlorophyceaePlant Sciencelcsh:Plant culturelevätlipiditbiomolekyylit010603 evolutionary biology01 natural sciencesbiomolecules4-METHYL STEROLSlipidsAlgaeMIXING MODELPhytoplanktonBotanylcsh:SB1-111014. Life underwaterfreshwater1183 Plant biology microbiology virologyOriginal Researchchemistry.chemical_classificationalgaebiologySTEROL COMPOSITIONSTRAINS010604 marine biology & hydrobiologyfungita1183DinoflagellateCYANOBACTERIAbiology.organism_classification6. Clean waterSterolDAPHNIA-MAGNAPERMANOVAchemotaxonomychemistryChemotaxonomyGROWTHGREEN-ALGAElipids (amino acids peptides and proteins)PERMDISPDinophyceaePolyunsaturated fatty acidFrontiers in Plant Science
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Allochthony, fatty acid and mercury trends in muscle of Eurasian perch (Perca fluviatilis) along boreal environmental gradients

2022

Environmental change, including joint effects of increasing dissolved organic carbon (DOC) and total phosphorus (TP) in boreal northern lakes could potentially affects food web energy sources and the biochemical composition of organisms. These environmental stressors are enhanced by anthropogenic land-use and can decrease the quality of polyunsaturated fatty acids (PUFAs) in seston and zooplankton, and therefore, possibly cascading up to fish. In contrast, the content of mercury in fish increases with lake browning potentially amplified by intensive forestry practises. However, there is little evidence on how these environmental stressors simultaneously impact beneficial omega-3 fatty acid …

LAKESTERRESTRIALliuennut orgaaninen hiiliEnvironmental EngineeringPHHYDROGEN ISOTOPESelohopeaAQUATIC FOOD WEBSEnvironmental changeHazard quotientFISHSTABLE-ISOTOPESAnimalsEnvironmental ChemistryDISSOLVED ORGANIC-CARBONahvenWaste Management and Disposal1172 Environmental sciencesFRESH-WATERNUTRITIONAL QUALITYMusclesrehevöityminenFatty AcidsvesiekosysteemitPhosphorusMercuryEutrophicationPollutionomegarasvahapotPerches1181 Ecology evolutionary biologyFatty Acids UnsaturatedAutochthonyBrowningympäristönmuutoksetravintoverkot
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