Search results for "adoptive"

Cutting Edge: An IL-17F-CreEYFP Reporter Mouse Allows Fate Mapping of Th17 Cells

2009

Abstract The need for reporter lines able to faithfully track Th17 cells in vivo has become an issue of exceptional importance. To address this, we generated a mouse strain in which Cre recombinase is expressed from the IL-17F promoter. Crossing the IL-17F-Cre allele to a conditional enhanced yellow fluorescent protein (EYFP) reporter mouse yielded the IL-17F-CreEYFP strain, in which IL-17F expression is twinned with EYFP in live IL-17F-expressing cells. Although we demonstrate that IL-17F expression is restricted to CD4+ T cells during experimental autoimmune encephalomyelitis, IL-17F-CreEYFP CD8 T cells robustly expressed IL-17F in response to TGF-β, IL-6, and IL-23. Fate mapping of IL-17…

L'adozione nel contesto e co-testo scuola. Literature review su adozione-scuola con Atlas.ti

Our research wants to be a meta-analysis work; its purpose is to focus on adoption and to analyse the “textual-semantic correlates” through which this topic has been understood and interpreted by international literature. In particular, the aim is to describe and codify the semantic-linguistic treatment of “adoption of children” theme with reference to school adjustment issue. In the title of the following thesis, “Adoption in the school context and co-text”, the term co-text (stemming from linguistics) points out the inquiry function in texts and the purpose of meta-reflective comparison among texts of articles coming from the journals with a high impact factor. After selecting those journ…

Holden Lori with Hass Crystal: The open-hearted way to open adoption. Helping your child growing up whole

2015

An open adoption is a way of keeping in contact with the biological roots, maybe more suitable for children adopted in their early infancy than later. In fact, children in older adoption age, coming from a pre-adoptive adverse environment (abuse, neglect, violence, poverty, etc.), may invoke oblio right ,tore-create themselves in the new family.

Methodological findings in studies on adoptive families

2014

The issue of adoptive families is the object of interest for many researchers, especially outside Polish borders. A critical analysis of previous studies on adoption shows that the scientific value of many of the existing studies raises doubts. The reasons of this are more or less significant methodological weaknesses, which significantly reduce their scientific value. Researchers often focus on the study of individuals in the adoptive family, e.g. mothers only, adopted children only or selected types of relationships within the family e.g. the mother – child relation. There is a little amount of studies on family systems. It seems worthy to note that numerous studies are conducted in the c…

Smad7 controls resistance of colitogenic T cells to regulatory T cell-mediated suppression.

2008

Background & Aims Foxp3-expressing regulatory T cells (Tregs) play a key role in the maintenance of the gut immune homeostasis, and an intact transforming growth factor (TGF)-β signaling is required for their function. In inflammatory bowel disease (IBD), the TGF-β signaling is impaired because of high expression of the inhibitory molecule Smad7. Although no intrinsic defects in Tregs function have been shown in IBD, it is still unknown whether colitogenic T cells are susceptible to Treg-mediated suppression. In this study, we have investigated whether IBD mucosal CD4+ T cells are resistant to Tregs and whether Smad7 is involved in this process. Methods IBD lamina propria mononuclear cells …

T-Cell Vaccination in Patients with Nickel Allergic Contact Dermatitis

1995

Immunotherapy targeting colon cancer stem cells

2010

In the last 10 years, cancer stem cells have interested the scientific community because this small tumorigenic population is also associated with tumor progression in human patients and specific targeting of cancer stem cells could be a strategy to eradicate cancers currently resistant to conventional therapy. Clinical studies have recently demonstrated that adding immune therapy to chemotherapy has survival benefits in comparison with chemotherapy alone that can sensitize tumors to immune cell-mediated killing (e.g., increasing sensitivity of tumor cells to subsequent cytotoxicity by T cells via upregulation of death receptors DR5 and Fas). However, loss of MHC molecules is often observe…

Molecular Design of the Cαβ Interface Favors Specific Pairing of Introduced TCRαβ in Human T Cells

2008

Abstract A promising approach to adoptive transfer therapy of tumors is to reprogram autologous T lymphocytes by TCR gene transfer of defined Ag specificity. An obstacle, however, is the undesired pairing of introduced TCRα- and TCRβ-chains with the endogenous TCR chains. These events vary depending on the individual endogenous TCR and they not only may reduce the levels of cell surface-introduced TCR but also may generate hybrid TCR with unknown Ag specificities. We show that such hybrid heterodimers can be generated even by the pairing of human and mouse TCRα- and TCRβ-chains. To overcome this hurdle, we have identified a pair of amino acid residues in the crystal structure of a TCR that …

Retrieval of functional TCRs from single antigen-specific T cells: Toward individualized TCR-engineered therapies

2015

We have developed a highly versatile platform for the systematic retrieval of T-cell receptors (TCRs) from single-antigen-reactive T cells and for characterization of their function and specificity. This approach enables rapid extraction of multiple TCRs from repertoires in individuals and not only broadens the diversity of TCRs suitable for clinical use, but also sets the stage for actively personalized immunotherapeutic strategies.

Effects of B-Cell Lymphoma on the Immune System and Immune Recovery after Treatment: The Paradigm of Targeted Therapy

2022

B-cell lymphoma and lymphoproliferative diseases represent a heterogeneous and complex group of neoplasms that are accompanied by a broad range of immune regulatory disorder phenotypes. Clinical features of autoimmunity, hyperinflammation, immunodeficiency and infection can variously dominate, depending on the immune pathway most involved. Immunological imbalance can play a role in lymphomagenesis, also supporting the progression of the disease, while on the other hand, lymphoma acts on the immune system to weaken immunosurveillance and facilitate immunoevasion. Therefore, the modulation of immunity can have a profound effect on disease progression or resolution, which makes the immune syst…