Search results for "auxin"
showing 10 items of 81 documents
Influence of culture conditions on embryo formation and maturation in auxin-induced embryogenic cultures of Digitalis obscura.
1994
Somatic embryogenesis was induced in hypocotyls of Digitalis obscura using indoleacetic acid or 2,4-dichlorophenoxyacetic acid with different culture and subculture conditions. Indoleacetic acid-induced embryogenic cultures were used to investigate the effects of amino acids, polyamines and growth regulators on embryo differentiation and maturation. Supplementation of the media with amino acids, polyamines or abscisic acid did not influence or had an adverse effect on embryogenic response. Gibberellic acid at 1.4 μM in either culture (30 days) or subculture medium was effective in promoting both differentiation and normal embryo development. The efficiency of somatic embryogenesis was great…
Adventitious shoot regeneration from hypocotyl cultures of service tree(Sorbus domesticaL.)
1992
Effects of growth regulators and N03:NH4 ratio were investigated to determine the mor- phogenic capacity of hypocotyl explants of service tree (Sorbus domestica L.) Organogenic responses were preceded by an initial callus phase and depended mainly on the auxin type used. NAA induced root differentiation whereas IAA added to media containing BA promoted adventitious shoot regeneration. Best results were obtained with 2.5 μM each of BA and IAA (19% of caulogenic explants). This reponse was increased (up to 44%) by varying nitrate to ammonium ratio from 2:1 to 4:1.
Micropropagation of juvenile and adult Sorbus domestica L.
1991
Successful propagation of seedlings and mature trees of Sorbus domestica L. has been achieved by in vitro methods. Multiple shoot formation was obtained by placing shoot apices or nodal segments on a modified Schenck and Hildebrandt medium containing benzyladenine. Regenerated shoots were excised and induced to root on media with auxin. In the best treatments 75–85% of shoots from juvenile material rooted. Rooting capacity of shoots from mature explants was lower (30%) and was not improved by dipping the base of shoots in concentration solutions of indolebutyric or naphthaleneacetic acids. Plantlets were ultimately established in soil.
Differential Expression of theS-Adenosyl-l-Methionine Synthase Genes during Pea Development1
1998
Abstract Two genes coding for S-adenosyl-l-methionine synthase (SAMS, EC 2.5.1.6) were previously isolated from pea (Pisum sativum) ovaries. Both SAMS genes were highly homologous throughout their coding regions but showed a certain degree of sequence divergence within the 5′ and the 3′ untranslated regions. These regions have been used as gene-specific probes to analyze the differential expression of SAMS1and SAMS2 genes in pea plants. The ribonuclease protection assay revealed different expression patterns for each individual gene. SAMS1 was strongly expressed in nearly all tissues, especially in roots. SAMS2 expression was weaker, reaching its highest level at the apex. Following pollina…
Cutting Type and IBA Treatment Duration Affect <i>Teucrium fruticans</i> Adventitious Root Quality
2014
Root development of stem cuttings of Silver Germander (Teucrium fruticans) was investigated in relation to cutting type and indole-3-butyric acid (IBA) treatment. Terminal cuttings of a clone grown in Sicily were trimmed to three types: terminal cuttings with apex (TWA), terminal cuttings without apex (TWOA) or sub-terminal cuttings (ST). To verify the cutting response to exogenous auxin, cuttings were dipped to a 2.0 cm depth in a 0.5% indole-3-butyric acid solution for 0, 5 or 7 minutes. Overall percent survival was 97 to 98%. Rooting percent, root number and root length were affected by cutting type and indole-3-butyric acid treatment. In general, TWA cuttings demonstrated a higher capac…
Demonstration and study of characters of foliar « compensatory growth» phenomenon in grapevine (<em>Vitis vinifera</em> L.)
1996
<p style="text-align: justify;">Defoliation and decapitation experiments showed that the growth of a grapevine leaf was inhibited by both the terminal bud and the younger higher leaves. There is a phenomenon of synergy between these two influences. A young leaf freed of these two influences showed a higher growth rate than the norm ; this is « compensatory growth ». It is when a leaf is in its phase of maximal growth that it is most able to realize this &amp;laquo compensatory growth ». This is not due to a longer growth time, but more to a faster growth rate. This « compensatory growth » is not the consequence of competition between leaf growth and internodal elongation. Exogenou…
Somatic embryogenesis and plant regeneration in a woody species: the European Spindle Tree (Euonymus europaeus L.).
1993
Somatic embryogenesis and subsequent plant regeneration of Euonymus europaeus L (European Spindle Tree) were obtained from square pieces of mature zygotic embryos with an intervening callus phase. Callus and somatic embryos were induced using a Murashige and Skoog's semi-solid basal medium supplemented with several combinations of auxins and cytokinins. The greatest number of somatic embryos was obtained with a continuous exposure to 22.8 μM indoleacetic acid and 0.046 μM kinetin. The frequency of somatic embryogenesis from zygotic embryos depends on the cold conservation time of seeds. The embryos frequently germinated on the same medium. Further development of somatic embryos into plantle…
The apparent loss of tissue culture competence during leaf differentiation in yams (Dioscorea bulbifera L.)
1993
Explants taken from the leaves of yams (Dioscorea bulbifera L.) at different stages of development were cultured in vitro on a checkerboard using various combinations and/or concentrations of auxin (2,4-d) and cytokinin (6-BAP). An addition of cytokinin to the culture media was not essential for callus induction from explants derived from young leaves in the very early stages of expansion. When the leaves expanded further they required cytokinin and the requirement increased considerably during expansion. Explants taken from fully expanded leaves were no longer able to proliferate, even when extremely high concentrations of cytokinins were applied. Callus grown from highly immature leaves w…
Effects of Gibberellic Acid on Morphogenesis and Cardenolide Accumulation in Juvenile and Adult Digitalis obscura Cultures
1993
Summary Effects of gibberellic acid on morphogenesis and cardenolide accumulation in in vitro cultures of Digitalis obscura are reported. Cultures were established from hypocotyls or leaves of axenic seedlings and shoot tips of mature plants. Irrespective of the kind of explant, gibberellic acid by itself did not induce morphogenesis but modified those morphogenic responses promoted by auxins and/or cytokinins, always inhibited organogenesis and favoured both embryo formation and conversion into plants. Gibberellic acid did not significantly affect cardenolide content of plants regenerated from juvenile or mature D. obscura explants.
Axillary shoot proliferation in cultures of explants from mature Juniperus oxycedrus trees.
1995
We developed procedures for the micropropagation of Juniperus oxycedrus L. using shoot apices or nodal segments from mature plants. Of the media and explants examined, best culture establishment was obtained with shoot apices cultured on modified Schenk and Hildebrandt medium (SH medium) without growth regulators; however, shoot multiplication was only achieved when shoot apices isolated from shoots grown on SH medium without growth regulators were subcultured on SH medium containing 0.5 micro M benzyladenine. None of the auxins and methods tested for root induction provided satisfactory results.