Search results for "biotecnología"
showing 10 items of 22 documents
Bifunctional poly(acrylamide) hydrogels through orthogonal coupling chemistries
2019
Biomaterials for cell culture allowing simple and quantitative presentation of instructive cues enable rationalization of the interplay between cells and their surrounding microenvironment. Poly(acrylamide) (PAAm) hydrogels are popular 2D-model substrates for this purpose. However, quantitative and reproducible biofunctionalization of PAAm hydrogels with multiple ligands in a trustable, controlled, and independent fashion is not trivial. Here, we describe a method for bifunctional modification of PAAm hydrogels with thiol- and amine- containing biomolecules with controlled densities in an independent, orthogonal manner. We developed copolymer networks of AAm with 9% acrylic acid and 2% N-(4…
Biological activities of polyphenols-enriched propolis from Argentina arid regions
2015
Background: Propolis is a bioactive natural product collected by honeybees (Apis mellifera) fromplant sources. Purpose: This studywas undertaken to determine the effect of propolis extracts from arid region of Argentina, on the activity/expression of pro-inflammatory enzymes, and as potential free radical scavenger, antifungal and anthelmintic agent as well as to get a first insight into the polyphenolic profile of the active fractions.Study design/methods: Two propolis samples were collected in different time from hives located in Tucumán, Argentina. They are representative of the collection time of the raw material for phytotherapeutical purposes. Ethanolic extracts from both propolis wer…
Microenvironments to study migration and somal translocation in cortical neurons
2018
Migrating post-mitotic neurons of the developing cerebral cortex undergo terminal somal translocation (ST) when they reach their final destination in the cortical plate. This process is crucial for proper cortical layering and its perturbation can lead to brain dysfunction. Here we present a reductionist biomaterials platform that faithfully supports and controls the distinct phases of terminal ST in vitro. We developed microenvironments with different adhesive molecules to support neuronal attachment, neurite extension, and migration in distinct manners. Efficient ST occurred when the leading process of migratory neurons crossed from low-to high-adhesive areas on a substrate, promoting spr…
In Situ, Light-Guided Axon Growth on Biomaterials via Photoactivatable Laminin Peptidomimetic IK(HANBP)VAV
2018
The ability to guide the growth of neurites is relevant for reconstructing neural networks and for nerve tissue regeneration. Here, a biofunctional hydrogel that allows light-based directional control of axon growth in situ is presented. The gel is covalently modified with a photoactivatable derivative of the short laminin peptidomimetic IKVAV. This adhesive peptide contains the photoremovable group 2-(4′-amino-4-nitro-[1,1′-biphenyl]-3-yl)propan-1-ol (HANBP) on the Lys rest that inhibits its activity. The modified peptide is highly soluble in water and can be simply conjugated to -COOH containing hydrogels via its terminal -NH 2 group. Light exposure allows presentation of the IKVAV adhesi…
Saccharomyces uvarum is responsible for the traditional fermentation of apple chicha in Patagonia
2016
Apple chicha is a fresh low alcoholic beverage elaborated by aboriginal communities of Andean Patagonia (Argentina and Chile). In the present work, we identified the yeast microbiota associated with this fermentation, and characterized genetically those belonging to the genus Saccharomyces. Both Saccharomyces cerevisiae and S. uvarum were found in the analyzed fermentations. Phylogenetic and population structure analyses based on genes sequence analysis were carried out for both S. cerevisiae and S. uvarum strains obtained in this study and a set of additional strains from diverse origins. The results demonstrate that S. cerevisiae strains from apple chicha belong to the big group of wine/E…
Torulaspora delbrueckii: un modelo para el estudio del estrés por NaCl en levadura de panaderia.
2005
Los hábitos alimenticios del hombre han variado en estos últimos años, aumentando la demanda de productos congelados de panadería y bollería por lo que se requieren levaduras con gran resistencia a diferentes tipos de estrés. En la masa panaria, la presencia de NaCl provoca por una parte un estrés osmótico debido a la pérdida masiva de agua de la célula y por otra parte un estrés iónico debido a las altas concentraciones de iones Na+ y Cl- en el citoplasma, lo que tiene efectos dañinos para la levadura.Torulaspora delbrueckii es una levadura capaz de fermentar una masa panaria y más resistente a estrés que la habitualmente utilizada en la industria, Saccharomyces cerevisiae. A pesar de cump…
Saccharomyces eubayanusandSaccharomyces uvarumassociated with the fermentation ofAraucaria araucanaseeds in Patagonia
2014
Mudai is a traditional fermented beverage, made from the seeds of the Araucaria araucana tree by Mapuche communities. The main goal of the present study was to identify and characterize the yeast microbiota responsible of Mudai fermentation as well as from A. araucana seeds and bark from different locations in Northern Patagonia. Only Hanseniaspora uvarum and a commercial bakery strain of Saccharomyces cerevisiae were isolated from Mudai and all Saccharomyces isolates recovered from A. araucana seed and bark samples belonged to the cryotolerant species Saccharomyces eubayanus and Saccharomyces uvarum. These two species were already reported in Nothofagus trees from Patagonia; however, this …
Desarrollo y aplicación de sistemas rápidos para la detección, indentificación y caracterización de levaduras alterantes de alimentos.
2006
La importancia de las levaduras se conoce desde hace mucho tiempo y con el desarrollo de la industria, se utilizan no sólo para la elaboración de un gran número de productos fermentados (cerveza, vino, quesos y embutidos), sino también para la producción de antibióticos, vitaminas, enzimas, etc. Sin embargo, las levaduras tienen también un aspecto negativo, debido a su potencial como alterantes de alimentos, lo que implica pérdidas económicas importantes para las industrias. En el presente trabajo se abordan aspectos relacionados con la identificación y caracterización molecular de algunas de las especies típicamente alterantes, pertenecientes a los géneros Debaryomyces, Zygosaccharomyces, …
Response to long-term NaHCO3-derived alkalinity in model Lotus japonicus Ecotypes Gifu B-129 and Miyakojima MG-20: transcriptomic profiling and physi…
2014
The current knowledge regarding transcriptomic changes induced by alkalinity on plants is scarce and limited to studieswhere plants were subjected to the alkaline salt for periods not longer than 48 h, so there is no information availableregarding the regulation of genes involved in the generation of a new homeostatic cellular condition after long-termalkaline stress.Lotus japonicusis a model legume broadly used to study many important physiological processes includingbiotic interactions and biotic and abiotic stresses. In the present study, we characterized phenotipically the response toalkaline stress of the most widely usedL. japonicusecotypes, Gifu B-129 and MG-20, and analyzed global t…
Improvement of baculovirus as protein expression vector and as biopesticide by CRISPR/Cas9 editing
2019
The clustered regularly interspaced short palindromic repeats (CRISPR) system?associated Cas9 endonuclease is a molecular tool that enables specific sequence editing with high efficiency. In this study, we have explored the use of CRISPR/Cas9 system for the engineering of baculovirus. We have shown that the delivering of Cas9-single guide RNA ribonucleoprotein (RNP) complex with or without DNA repair template into Sf21 insect cells through lipofection might be efficient to produce knockouts as well as knock-ins into the baculovirus. To evaluate potential application of our CRISPR/Cas9 method to improve baculovirus as protein expression vector and as biopesticide, we attempted to knockout se…