Search results for "carbapenems"

showing 10 items of 28 documents

OXA-163-Producing Klebsiella pneumoniae in Cairo, Egypt, in 2009 and 2010

2012

ABSTRACT Two genetically unrelated OXA-163-carrying Klebsiella pneumoniae strains were identified from two infection cases in June 2009 and May 2010 in Cairo, Egypt. OXA-163-producing Enterobacteriaceae had been previously reported in Argentina only. Both patients had no history of travel abroad. The emergence of this newly recognized OXA-48-related β-lactamase able to hydrolyze cephalosporins and carbapenems is especially worrying in a geographic area where OXA-48 is endemic and effective surveillance for antibiotic resistance is largely unaffordable.

AdultDNA BacterialMaleMicrobiology (medical)Settore MED/07 - Microbiologia E Microbiologia ClinicaOXA-163EpidemiologyKlebsiella pneumoniaemedicine.drug_classMolecular Sequence DataCephalosporinSettore MED/42 - Igiene Generale E Applicatabeta-Lactam Resistancebeta-LactamasesTravel abroadMicrobiologyAntibiotic resistancepolycyclic compoundsmedicineHumansOXA-163; Klebsiella pneumoniaebiologyGeographic areaHydrolysisSequence Analysis DNAKlebsiella infectionsbiochemical phenomena metabolism and nutritionbiology.organism_classificationVirologyAnti-Bacterial AgentsCephalosporinsKlebsiella InfectionsKlebsiella pneumoniaeCarbapenemsbacteriaEgyptFemaleJournal of Clinical Microbiology
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Carbapenem-Susceptible OXA-23-Producing Proteus mirabilis in the French Community

2019

International audience; Nineteen Proteus mirabilis isolates producing the carbapenemase OXA-23 were recovered over a 2-year period in 19 French hospitalized patients, of whom 12 had community onset infections. The isolates exhibited a slightly reduced susceptibility to carbapenems. Whole-genome analysis revealed that all 19 isolates formed a cluster compared to 149 other P. mirabilis isolates. Because of its susceptibility to carbapenems, this clone may be misidentified as a penicillinase producer while it constitutes a reservoir of the OXA-23-encoding gene in the community.

CarbapenemHospitalized patientsspreadclonalityMicrobial Sensitivity Testsbeta-LactamasesEpidemiology and SurveillanceMicrobiology03 medical and health sciencescarbapenemasemedicinepolycyclic compoundsHumansPharmacology (medical)Proteus mirabilis030304 developmental biologyCommunity onsetPharmacology0303 health sciencesbiologyOXA-23030306 microbiologybiochemical phenomena metabolism and nutritionbiology.organism_classificationProteus mirabilisAnti-Bacterial AgentsInfectious DiseasesReduced susceptibility[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyCarbapenemsbacteriaFranceProteus Infectionsmedicine.drug
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An Update of the Evolving Epidemic of blaKPC Carrying Klebsiella pneumoniae in Sicily, Italy, 2014: Emergence of Multiple Non-ST258 Clones

2015

Background: In Italy, Klebsiella pneumoniae carbapenemase producing K. pneumoniae (KPC-Kp) strains are highly endemic and KPC producing CC258 is reported as the widely predominating clone. In Palermo, Italy, previous reports have confirmed this pattern. However, recent preliminary findings suggest that an epidemiological change is likely ongoing towards a polyclonal KPC-Kp spread. Here we present the results of molecular typing of 94 carbapenem non susceptible K. pneumoniae isolates detected during 2014 in the three different hospitals in Palermo, Italy. Methods and Results: Ninety-four consecutive, non replicate carbapenem non susceptible isolates were identified in the three largest acute…

CarbapenemKlebsiella pneumoniaelcsh:MedicineGene ExpressionDrug resistancePlasmidbeta-LactamaseDisease OutbreaksMolecular typingFluoroquinoloneDrug Resistance Multiple Bacterialpolycyclic compoundslcsh:ScienceCarbapenemMembrane ProteinDisease OutbreakMultidisciplinarybiologyMedicine (all)IncidenceHospitalsAnti-Bacterial AgentsElectrophoresis Gel Pulsed-FieldKlebsiella pneumoniaeItalyEpidemiological MonitoringHumanFluoroquinolonesPlasmidsResearch Articlemedicine.drugBacterial ProteinAminoglycosides; Anti-Bacterial Agents; Bacterial Proteins; Carbapenems; Clone Cells; Colistin; Drug Resistance Multiple Bacterial; Electrophoresis Gel Pulsed-Field; Epidemiological Monitoring; Fluoroquinolones; Gene Expression; Hospitals; Humans; Incidence; Italy; Klebsiella Infections; Klebsiella pneumoniae; Membrane Proteins; Multilocus Sequence Typing; Mutation; Plasmids; beta-Lactamases; Disease Outbreaks; Agricultural and Biological Sciences (all); Biochemistry Genetics and Molecular Biology (all); Medicine (all)beta-LactamasesMicrobiologyClone CellHospitalAntibiotic resistanceBacterial ProteinsAnti-Bacterial AgentmedicineHumansBiochemistry Genetics and Molecular Biology (all)AminoglycosideColistinlcsh:RMembrane ProteinsCarbapenemase producingbiochemical phenomena metabolism and nutritionbacterial infections and mycosesbiology.organism_classificationVirologyClone CellsKlebsiella InfectionsAminoglycosidesAgricultural and Biological Sciences (all)CarbapenemsMutationColistinMultilocus sequence typinglcsh:QKlebsiella InfectionMultilocus Sequence TypingPLOS ONE
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Characterization of the carbapenem-hydrolyzing oxacillinase OXA-58 in an Acinetobacter phenon 6/ct13TU clinical isolate

2008

The bla(OXA-58) gene identified in the Acinetobacter phenon 6/ct13TU clinical isolate presented 100% homology with the same gene in Acinetobacter baumannii. Its location in a plasmid suggests that these resistance genes may be transferred from 1 species to another.

DNA BacterialMicrobiology (medical)CarbapenemGene Transfer HorizontalSequence HomologyBiologybeta-LactamasesHomology (biology)MicrobiologyAntibiotic resistancePlasmidmedicineHumansElectrophoresis Agar GelAcinetobacterNucleic Acid HybridizationSequence Analysis DNAGeneral Medicinebiochemical phenomena metabolism and nutritionAcinetobacterbacterial infections and mycosesbiology.organism_classificationAcinetobacter baumanniiInfectious DiseasesCarbapenemsGenes BacterialNeisseriaceaeBacteriaAcinetobacter InfectionsPlasmidsmedicine.drugDiagnostic Microbiology and Infectious Disease
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A novel VIM‐type metallo‐beta‐lactamase (VIM‐14) in a Pseudomonas aeruginosa clinical isolate from a neonatal intensive care unit

2011

AbstractA Pseudomonas aeruginosa highly resistant to carbapenems was isolated in a neonatal intensive care unit in Palermo, Italy. The strain was found to carry a novel VIM‐type enzyme, classified as VIM‐14. The novel enzyme differs from VIM‐4 in a G31S mutation. VIM‐14 was harboured in a class 1 integron with a new organization. The integron carried the genes aac7, blaVIM‐14, blaOXA‐20 and aac4 in that order.

DNA BacterialMicrobiology (medical)Settore MED/07 - Microbiologia E Microbiologia ClinicaNeonatal intensive care unitSettore MED/17 - Malattie Infettivemetallo-b-lactamaseAntibiotic resistancemetallo-β-lactamasemedicine.medical_treatmentMolecular Sequence DataMicrobial Sensitivity TestsBiologySettore MED/42 - Igiene Generale E Applicatamedicine.disease_causeIntegronbeta-LactamasesIntegronscarbapenemlaw.inventionMicrobiologyAntibiotic resistancelawDrug Resistance Multiple BacterialIntensive Care Units Neonatalpolycyclic compoundsmedicineHumansVIM-14Antibacterial agentBase SequencePseudomonas aeruginosaInfant Newbornmetallo‐β‐lactamaseAntibiotic resistance; carbapenems; metallo-b-lactamase; Pseudomonas aeruginosa; VIM-14Sequence Analysis DNAGeneral Medicinebiochemical phenomena metabolism and nutritionbacterial infections and mycosesbiology.organism_classificationIntensive care unitInfectious DiseasesPseudomonas aeruginosaBeta-lactamasebiology.proteinbacteriacarbapenemsVIM‐14PseudomonadaceaeClinical Microbiology and Infection
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Ongoing spread of colistin-resistant Klebsiella pneumoniae in different wards of an acute general hospital, Italy, June to December 2011.

2012

We describe polyclonal spread of colistin-resistant Klebsiella pneumoniae in an acute general hospital in Italy. Between June and December 2011, 58 colistin-resistant K. pneumoniae isolates were recovered from 28 patients admitted to different wards, but mainly in the intensive care units. All isolates were tested for drug susceptibility and the presence of beta-lactamase (bla) genes. Clonality was investigated by repetitive extragenic palindromic (rep)-PCR and multilocus sequence typing (MLST). Fifty-two isolates had minimum inhibitory concentrations (MICs) for colistin of 6-128 mg/L, carried blaKPC3 and were attributed to sequence type ST258. The remaining six isolates were susceptible to…

DNA BacterialSettore MED/07 - Microbiologia E Microbiologia ClinicaEpidemiologyKlebsiella pneumoniaeMicrobial Sensitivity TestsSettore MED/42 - Igiene Generale E ApplicataHospitals GeneralPolymerase Chain ReactionKlebsiella pneumoniae carbapenems colistin resistance ICU epidemiologybeta-LactamasesMicrobiologyDisease OutbreaksAntibiotic resistanceVirologyIntensive careDrug Resistance Multiple BacterialPatients' RoomsMedicineHumansKlebsiella pneumoniae; colistin-resistance; MLSTGeneral hospitalCross Infectionbiologybusiness.industryColistinPublic Health Environmental and Occupational HealthOutbreakbiochemical phenomena metabolism and nutritionbiology.organism_classificationAnti-Bacterial AgentsBacterial Typing TechniquesKlebsiella InfectionsIntensive Care UnitsKlebsiella pneumoniaeCarbapenemsItalyColistinMultilocus sequence typingbusinessHorizontal transmissionmedicine.drugMultilocus Sequence TypingEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin
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Characterization of the Carbapenem-Hydrolyzing Oxacillinase Oxa-58 in an Acinetobacter Genospecies 3 Clinical Isolate

2008

ABSTRACT Based on imipenem resistance in an Acinetobacter genospecies 3 clinical isolate, we were able to identify, for the first time in this genomic species, a plasmid-encoded bla OXA-58 gene that was 100% homologous to the same gene in Acinetobacter baumannii .

ImipenemCarbapenemMolecular Sequence DataMicrobial Sensitivity TestsBiologybeta-LactamasesMicrobiologyPlasmidBacterial ProteinsMechanisms of Resistancepolycyclic compoundsmedicineHumansPharmacology (medical)Antibacterial agentPharmacologyAcinetobacterBase SequenceSequence Analysis DNAbiochemical phenomena metabolism and nutritionAcinetobacterbacterial infections and mycosesbiology.organism_classificationAnti-Bacterial AgentsAcinetobacter baumanniiImipenemInfectious DiseasesCarbapenemsNeisseriaceaeBacteriaAcinetobacter InfectionsPlasmidsmedicine.drugAntimicrobial Agents and Chemotherapy
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Outbreak of IMI-1 Carbapenemase-producing colistin-resistant Enterobacter cloacae on the French island of Mayotte (Indian Ocean)

2018

International audience; The spread of carbapenemase-producing Enterobacteriaceae in the Southwest Indian Ocean islands is poorly known. Here we describe an outbreak of colistin-resistant Enterobacter cloacae harbouring blaIMI-1 in the French overseas department of Mayotte. Between October 2015 and January 2017, all isolates of imipenem-non-susceptible E. cloacae at Mayotte Medical Center and University Hospital of Reunion Island were screened for carbapenemase production. Positive isolates were typed by pulsed-field gel electrophoresis and whole-genome sequencing (WGS)-based multilocus sequence typing (MLST), and all β-lactamase genes were identified by PCR and sequencing. Resistance profil…

Male0301 basic medicineImipenembla(IMI-1)Carbapenem-resistant enterobacteriaceaeComorosDisease Outbreaks[ SDV.MP ] Life Sciences [q-bio]/Microbiology and ParasitologyPharmacology (medical)Enterobacteriaceae InfectionsGeneral MedicineMiddle AgedFrench overseas islandAnti-Bacterial AgentsElectrophoresis Gel Pulsed-Field3. Good healthInfectious Diseases[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyFemalemedicine.drugAdultErtapenemMicrobiology (medical)Adolescent030106 microbiologyMayotteMicrobial Sensitivity TestsBiologybeta-LactamasesMicrobiologyCarbapenemaseYoung Adult03 medical and health sciencesBacterial Proteins[ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathologyEnterobacter cloacaePulsed-field gel electrophoresismedicineHumansEtestColistinOutbreakOutbreakbiology.organism_classificationCephalosporinsImipenemCarbapenem-Resistant EnterobacteriaceaeCarbapenemsColistinMultilocus sequence typingEnterobacter cloacaeGenome BacterialMultilocus Sequence Typing
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Epidemiology and clonality of carbapenem-resistant Acinetobacter baumannii from an intensive care unit in Palermo, Italy

2012

Abstract Background Multidrug-resistant Acinetobacter baumannii, initially considered as having a poor clinical relevance, is frequently isolated from infection cases in intensive care units. We describe the epidemiology of carbapenem resistant A. baumannii (CRAB) in a general ICU in Palermo, Italy, from October 2010 to March 2011. Findings 58 of 61 isolates exhibited MICs for meropenem or imipenem ≥16 mg/L. Forty-nine carried blaOXA-23 and two blaOXA-58 genes. Five subtype clusters were detected by rep-PCR. Clusters D and E included 10 isolates that tested negative for the carbapenem resistance genes. MLST attributed all isolates, but two, with sequence type (ST)2, whereas the two remainin…

MaleAcinetobacter baumanniiImipenemSettore MED/07 - Microbiologia E Microbiologia ClinicaTime Factorslcsh:MedicineTigecyclinePolymerase Chain Reactionintensive care unitlaw.inventionlawDrug Resistance Multiple BacterialEpidemiologypolycyclic compoundsMedicinelcsh:QH301-705.5Medicine(all)Aged 80 and overbiologyGeneral MedicineMiddle AgedIntensive care unitAcinetobacter baumanniiIntensive Care UnitsItalyFemaleAcinetobacter baumannii; intensive care unitAcinetobacter Infectionsmedicine.drugAdultmedicine.medical_specialtyAdolescentShort ReportMicrobial Sensitivity TestsMeropenemGeneral Biochemistry Genetics and Molecular BiologyYoung AdultIntensive careHumansIntensive care medicinelcsh:Science (General)AgedDemographyBiochemistry Genetics and Molecular Biology(all)business.industrylcsh:Rbiochemical phenomena metabolism and nutritionbacterial infections and mycosesbiology.organism_classificationClone CellsCarbapenemslcsh:Biology (General)bacteriabusinessCarbapenem resistant Acinetobacter baumanniilcsh:Q1-390BMC Research Notes
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Characterization of Carbapenem-Resistant Enterobacteriaceae Clinical Isolates in Al Thawra University Hospital, Sana'a, Yemen

2020

International audience; Objective: The aim of this study was to investigate the resistance mechanisms of carbapenem-resistant Enterobacteriaceae clinical strains recovered from Al Thawra University Hospital, Sana'a, Yemen. Methods: A total of 27 isolates showing decreased susceptibility to carbapenems were obtained from different clinical specimens in Al Thawra Hospital, Sana'a, Yemen. Strains were identified by Matrix Assisted Laser Desorption Ionization Time-Of-Flight spectroscopy. Susceptibility to antibiotics was determined by the disk diffusion method on Mueller Hinton agar. Carbapenemases-encoding genes, extended-spectrum β-lactamases (ESBLs), and plasmid-mediated quinolone resistance…

MaleYemenKlebsiella pneumoniaeGene ExpressionCarbapenem-resistant enterobacteriaceaeQuinolonesmedicine.disease_causeHospitals Universitychemistry.chemical_compoundChildEscherichia coli InfectionsAged 80 and over0303 health sciencesbiologyEnterobacteriaceae InfectionsMiddle AgedEnterobacteriaceae3. Good healthAnti-Bacterial AgentsBacterial Typing TechniquesMueller-Hinton agarKlebsiella pneumoniae[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyNDM-1-likeChild PreschoolFemalePlasmidsMicrobiology (medical)AdultcarbapenemasesImmunologyMicrobial Sensitivity TestsMicrobiologybeta-Lactam Resistancebeta-LactamasesMicrobiology03 medical and health sciencesEnterobacteriaceaeEnterobacter cloacaemedicineEscherichia coliHumansTypingEscherichia coli030304 developmental biologyAgedPharmacology030306 microbiologyInfantbiochemical phenomena metabolism and nutritionbiology.organism_classificationClone CellsKlebsiella InfectionsCarbapenem-Resistant EnterobacteriaceaechemistryCarbapenemsMultilocus sequence typingbacteriaEnterobacter cloacaeMultilocus Sequence TypingOXA-48-like
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