Search results for "hate"

Speciation of Cr in Leachates of a MSWI Bottom Ash Landfill

1998

Cr concentrations and speciation were determined in leachate from a municipal solid waste incinerator bottom ash landfill both experimentally and by thermodynamic model calculations. Total dissolved Cr concentrations of 0.2 mmol L-1 were determined by GFAAS. Two orders of magnitude lower values were determined upon preconcentration by an in-situ solid-phase extraction technique based on the 8-HQ cation exchanger that is specific for Cr(III) but unspecific for Cr(VI). This suggests that chromate dominates the dissolved Cr concentrations in the leachates but was up to 5 orders of magnitude undersaturated with respect to the solubility of CaCrO4 or BaCrO4. Chromate adsorption by oxyhydroxides …

The inner nuclear membrane protein Src1 associates with subtelomeric genes and alters their regulated gene expression

2008

Inner nuclear membrane proteins containing a LEM (LAP2, emerin, and MAN1) domain participate in different processes, including chromatin organization, gene expression, and nuclear envelope biogenesis. In this study, we identify a robust genetic interaction between transcription export (TREX) factors and yeast Src1, an integral inner nuclear membrane protein that is homologous to vertebrate LEM2. DNA macroarray analysis revealed that the expression of the phosphate-regulated genes PHO11, PHO12, and PHO84 is up-regulated in src1Δ cells. Notably, these PHO genes are located in subtelomeric regions of chromatin and exhibit a perinuclear location in vivo. Src1 spans the nuclear membrane twice an…

Ultra-high-pressure liquid chromatography tandem mass spectrometry method for the determination of 9 organophosphate flame retardants in water samples

2016

Few methods are available for comprehensive organophosphate flame retardants (PFRs) detection in water and wastewater. Gas chromatography has been employed previously, but this approach is less selective, not amenable for use with deuterated standards and can suffer unfavorable fragmentation. Ultra-high-pressure liquid chromatography tandem mass spectrometry (UHPLC-QqQ-MS/MS) has become the most promising platform, already applied successfully for analysis of selected PFRs in some environmental matrices like water and wastewater. However, the presence of some interferences from the dissolvent, the equipment and the used materials should be taken into account. The procedure involves: The fir…

Determination of organic and inorganic anions in insect haemolymph and midgut contents by ion chromatography

1997

A simple and rapid ion-exchange chromatographic method with conductimetric detection for the determination of carboxylic acids (succinic, malic and citric) and inorganic anions (chloride, phosphate and sulphate) in haemolymph and midgut content of Lepidoptera larvae is provided. The mobile phase is 0.975 mM phthalic acid at pH 4.1. The procedure of sample extractions was simple. Comparison of the results with whose reported in the bibliography showed that the values were similar. The ion-exchange chromatographic–conductimetric detection method permits the analysis of various organic and inorganic anions in small biological samples.

Extraktionschromatographische trennung der freien porphyrine auf tri-n-butylphosphat-s?ulen im pH-gradienten

1969

Abstract The method described allows the separation of the free porphyrins on a tri-n-butyl phosphate (TBP) column by elution with a pH gradient. The prophyrins are eluted from the column at discrete pH values (xD) according to the number of carboxyl groups in the side chains. Separation of isomers could not be observed under the conditions used. The xD values indicate the centre of the pH-interval, in which the dissociation of the carboxyl groups occurs. This finding could be confirmed by spectro-photometric measurements. By combining the method proposed with the method of separation by extraction chromatography in the TBP/IN HCl partition system and the thin-layer technique in the 2,6-lut…

Determination of alternative and conventional chelating agents as copper(II) complexes by capillary zone electrophoresis—The first use of didecyldime…

2006

Abstract A capillary zone electrophoresis (CZE) method for analyzing 11 chelating agents [β-alaninediacetic acid (β-ADA), trans-1,2-diaminocyclohexane- N , N , N ′, N ′-tetraacetic acid (CDTA), diethylenetriaminepentaacetic acid (DTPA), ethylenediaminetetraacetic acid (EDTA), N -(2-hydroxyethyl)ethylenediamine- N , N ′, N ′-triacetic acid (HEDTA), N -(2-hydroxyethyl)iminodiacetic acid (HEIDA), iminodiacetic acid (IDA), methylglycinediacetic acid (MGDA), nitrilotriacetic acid (NTA), 1,3-diaminopropane- N , N , N ′, N ′-tetraacetic acid (PDTA) and triethylenetetraaminehexaacetic acid (TTHA)] as negatively charged copper(II) complexes has been established. Both conventional and alternative che…

Influence of sodium lauryl sulphate on the in vitro percutaneous absorption of compounds with different lipophilicity

1997

Abstract The effects of sodium lauryl sulphate (SLS) at different concentrations on the “in vitro” percutaneous absorption of a number of compounds (5-fluorouracil, antipyrine, pentoxifylline, salicylamide, 4-ethylaniline, 4-n-butylaniline and indomethacin) with a wide range of lipophilicity values (log POCT from −0.95 to 4.42) through rat skin were analysed. After pretreatment of the skin with SLS (1% and 5%, w/w) the permeability coefficients increased only for compounds having a log POCT

ChemInform Abstract: K3Cr2(PS4)3: A New Chromium Thiophosphate with a One-Dimensional [Cr2(PS4)3]3- Anion Chain.

2010

Cloning, purification, and nucleotide-binding traits of the catalytic subunit A of the V1VO ATPase from Aedes albopictus.

2007

The Asian tiger mosquito, Aedes albopictus, is commonly infected by the gregarine parasite Ascogregarina taiwanensis, which develops extracellularly in the midgut of infected larvae. The intracellular trophozoites are usually confined within a parasitophorous vacuole, whose acidification is generated and controlled by the V(1)V(O) ATPase. This proton pump is driven by ATP hydrolysis, catalyzed inside the major subunit A. The subunit A encoding gene of the Aedes albopictus V(1)V(O) ATPase was cloned in pET9d1-His(3) and the recombinant protein, expressed in the Escherichia coli Rosetta 2 (DE3) strain, purified by immobilized metal affinity- and ion-exchange chromatography. The purified prote…

Modulation of geraniol metabolism during alcohol fermentation

2004

J. Inst. Brew. 110(3), 213–219, 2004 Geraniol is the main monoterpenol present in Gewurtztraminer white grapes and in some red aromatic grapes from northern Italy. During fermentation, the geraniol concentration showed a dramatic drop and a small amount was transformed into linalool and -terpineol by an acid catalysed chemical reaction. Yeast are responsible for changing most of the geraniol to citronellol through enzymatic reactions. The final aroma of wine is due mainly to both the untransformed geraniol and the formed citronellol. In this study, competition between the geraniol-citronellol transformation and another metabolic process, in which according to our hypothesis sterol biosynthe…