Search results for "naval"

Conditions for the Enhancing Effect of Protease Inhibitors on the Concanavalin A Induced Thymidine Response of Murine Lymphocytes

1983

Incorporation of [<sup>3</sup>H]-thymidine – [<sup>3</sup>H]-TdR – into concanavalin A (Con A) stimulated murine splenocytes and thymocytes was found to be enhanced by addition of certain concentrations of phenyl-methyl-sulfonylfluoride (PMSF), di-isopropylfluorophosphate (DFP), N-α-tosyl-<i>L</i>-lysyl-<i>L</i>-chloromethylketone (TLCK), and soybean trypsin inhibitor (SBTI). No enhancement could be observed when mononuclear cells of the peripheral blood were used, and a medium enhancement when thymocytes were applied. Furthermore, no enhancing effect of the protease inhibitors (PI) on the Con A response of murine splenocytes could be observed…

Contact sensitizers modulate mechanisms of receptor-mediated endocytosis but not fluid-phase endocytosis in murine epidermal Langerhans cells.

1995

In order to define the influence of contact allergens on the fluid-phase endocytosis (FPE) of soluble molecules of murine epidermal Langerhans cells (LC), we studied the internalization of FITC-labeled bovine serum albumin (FITC-BSA), TRITC-labeled dextrane (TRITC-DEX) as well as horseradish peroxidase by LC. A 3-parameter flow-cytometric technique was performed for quantification of internalized FITC-BSA in LC using quantum red-labeled reagents for detection of la-antigen expression by LC and propidium iodide for exclusion of dead cells from analysis. A temperature-dependent rapid accumulation of FITC-BSA was noticed in time-course studies reaching a plateau between 1 and 2 h of in vitro c…

An approach to predictive testing of contact sensitizers in vitro by monitoring their influence on endocytotic mechanisms.

1996

Endocytotic activation of epidermal Langerhans cells (LC) by immunogenic haptens is an early event during development of allergic contact dermatitis. In this work a fast and objective flow-cytometric assay for predictive in vitro testing of contact sensitizers by monitoring their influence on endocytotic mechanisms in murine LC was developed. Epidermal cell suspensions were labelled with a monoclonal antibody directed to MHC class II molecules and pH-sensitive fluorochrome-coupled second-step reagents. For untreated LC a significant quenching of fluorescence intensity by internalization of the MHC-antibody complexes into acidic compartments was noticed. Similar results were obtained in the …

The role of accessory cells in polyclonal T cell activation. I. Both induction of interleukin 2 production and of interleukin 2 responsiveness by con…

1983

Recent studies from other laboratories have shown that concanavalin A (Con A) acts at two separate steps in polyclonal T cell activation: interleukin 2 (IL2) production, and induction of responsiveness to IL2. Using a combination of techniques for the depletion of accessory cells from lymph node T cells, we have investigated which of these steps, if not both, is responsible for the known requirement for accessory cells in the Con A response. It was found that with increasing T cell purification, first the ability is lost to produce sufficient levels of endogenous IL2, whereas induction of IL2 responsiveness can still take place. Further removal of accessory cells however yields a population…

Mascheramenti festivi in Sicilia

2022

I mascheramenti cerimoniali, che vanno dal semplice occultamento del viso al rivestimento del corpo con specifici indumenti e attributi, ricorrono più diffusamente, in Sicilia come altrove, nel periodo carnevalesco. A cortei e performances di mascherati si accompagnano comportamenti tesi a segnalare la temporanea sospensione o il rivolgimento delle norme sociali e culturali: i giuochi licenziosi, le danze pubbliche e i balli domestici, le questue e lo smodato consumo di peculiari cibi e di bevande, la produzione di rumori e di schiamazzi, le tenzoni, il dileggio delle autorità, i motteggi e gli scherzi d’ogni sorta. Tra le performances carnascialesche che meritano di essere ricordate: la pa…

Máscaras de Sicilia. Fotografías de Attilio Russo y Giuseppe Muccio

2022

I mascheramenti, al pari degli altri simboli rituali caratterizzanti le celebrazioni carnascialesche siciliane, paiono tutti riconducibili alla sintassi propria delle feste di chiusura e apertura di un ciclo ossia di rifondazione ciclica del tempo, e finiscono con il proporre l’istituto carnevalesco, nel suo complesso, come “modello esemplare” delle cerimonie di Capodanno. Una festa cioè che attraverso parole, immagini, gesti riesce a restituire la realtà attraverso la ricostituzione per simulazione del cerchio del tempo, cioè a garantire che il tempo continui ad esserci, simulandone e ripetendone la morte e la rinascita. Las mascaradas, al igual que los demás símbolos rituales que caracter…

Fatigue crack growth of new FML composites for light ship buildings under predominant mode II loading condition

2019

The use of light but strong materials is largely studied in various area of the shipbuilding, this because the need of reducing the weight, and especially the weight of all the structures above the main deck assume primary importance for the stability. Traditionally in fast boats like fast ferries, hydrofoils, patrol boats, the typical materials are Aluminum alloy or composites, both those materials have advantages and disadvantages, but the new development of technologies made possible to combine them, in order to have a new material, combining the advantages of both, in terms of fatigue resistance, firefighting characteristics. In this paper, predominant mode II fatigue delamination tests…

Oligomerization of Concanavalin A in live cells detected by fluctuation analysis.

2009

Biomolecular conjugation inside synthetic polymer nanopores via glycoprotein-lectin interactions

2011

We demonstrate the supramolecular bioconjugation of concanavalin A (Con A) protein with glycoenzyme horseradish peroxidase (HRP) inside single nanopores, fabricated in heavy ion tracked polymer membranes. Firstly, the HRP-enzyme was covalently immobilized on the inner wall of the pores using carbodiimide coupling chemistry. The immobilized HRP-enzyme molecules bear sugar (mannose) groups available for the binding of Con A protein. Secondly, the bioconjugation of Con A on the pore wall was achieved through its biospecific interactions with the mannose residues of the HRP enzyme. The immobilization of biomolecules inside the nanopore leads to the reduction of the available area for ionic tran…

Potential impact of a nonavalent HPV vaccine on HPV related low-and high-grade cervical intraepithelial lesions: A referral hospital-based study in S…

2017

ABSTRACT While bivalent and quadrivalent HPV vaccines have been used for about 10 years, a nonavalent vaccine against HPV types 6/11/16/18/31/33/45/52 and 58 has been recently approved by FDA and EMA and is now commercially available. The objective of our study was to evaluate the potential impact of the nonavalent vaccine on HPV infection and related low- and high-grade squamous intraepithelial lesions (LSIL, HSIL), compared to the impact of the quadrivalent vaccine, in a female population living in Sicily (Italy). Low estimates of HPV vaccine impact were calculated as prevalence of HPV 6/11/16/18/31/33/45/52 and 58 genotypes, alone or in association, but excluding presence of other HPV ty…