Search results for "oligo"
showing 10 items of 1298 documents
Influencia de los galactooligosacáridos sobre la biodisponibilidad y fermentación colónica de esteroles
2021
El enriquecimiento de alimentos con esteroles vegetales (EV) es una práctica común en el desarrollo de alimentos funcionales orientados a reducir el riesgo de enfermedades cardiovasculares debido a su conocido efecto hipocolesterolemiante. Las bebidas a base de zumo de frutas y leche son una matriz idónea para el enriquecimiento con estos compuestos bioactivos debido a su perfil nutricional saludable. Además, su efectividad se ha demostrado en poblaciones diana como mujeres postmenopáusicas con hipercolesterolemia moderada (población de interés al presentar un mayor riesgo de enfermedad cardiovascular). La adición de galactooligosacáridos (GOS) a estas bebidas podría mejorar su funcionalida…
Pharmacological activation of CB2 receptors counteracts the deleterious effect of ethanol on cell proliferation in the main neurogenic zones of the a…
2015
Chronic alcohol exposure reduces endocannabinoid activity and disrupts adult neurogenesis in rodents, which results in structural and functional alterations. Cannabinoid receptor agonists promote adult neural progenitor cell (NPC) proliferation. We evaluated the protective effects of the selective CB1 receptor agonist ACEA, the selective CB2 receptor agonist JWH133 and the fatty-acid amide-hydrolase (FAAH) inhibitor URB597, which enhances endocannabinoid receptor activity, on NPC proliferation in rats with forced consumption of ethanol (10%) or sucrose liquid diets for 2 weeks. We performed immunohistochemical and stereological analyses of cells expressing the mitotic phosphorylation of his…
[1,2,3]Triazolo[1,5-a]piridinas y compuestos relacionados : aplicaciones en química supramolecular : actividad biológica
2013
Los heterociclos nitrogenados son moléculas clave dentro de numerosos campos científicos. En concreto, los triazoles han encontrado numerosas aplicaciones en química médica como antifúngicos (fluconazol, posaconazol), antidepresivos (trazodona) o anticancerígenos (triazoloacridonas). En este contexto se decidió sintetizar nuevos derivados de [1,2,3]triazolo[1,5-a]piridinas con el fin de explorar su capacidad de interaccionar con ADN y su actividad leishmanicida y antichagásica. Con este objetivo se ha utilizado la química clásica de las triazolopiridinas (reacción de litiación regioselectiva de este heterociclo, seguida de adición de un electrófilo), para obtener nuevos derivados con estruc…
Safety and immunomodulatory effects of three probiotic strains isolated from the feces of breast-fed infants in healthy adults: SETOPROB study.
2013
We previously described the isolation and characterization of three probiotic strains from the feces of exclusively breast-fed newborn infants: Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036. These strains were shown to adhere to intestinal mucus in vitro, to be sensitive to antibiotics and to resist biliary salts and low pH. In the present study, a multicenter, randomized, double-blind, placebo-controlled trial with 100 healthy volunteers in three Spanish cities was carried out to evaluate the tolerance, safety, gut colonization and immunomodulatory effects of these three probiotics. Volunteers underwent a 15-day washout perio…
Selective downregulation of the MDR1 gene product in Caco-2 cells by stable transfection to prove its relevance in secretory drug transport.
2005
Considerable interest is focused on overcoming multidrug resistance (MDR) in cancer chemotherapy. The in vitro experiments to characterize P-glycoprotein's (P-gp) function and to decrease its effects have led to a variety of strategies such as addition of competitors or supplementation of the medium with oligonucleotides complementary to the 5'-end of the MDR1-mRNA. For the Caco-2 cell line, an in vitro model for absorption screening, expressing multiple transporters including P-gp, which pumps substances back into the apical solution, P-gp activity might mask other relevant transport proteins' activity. The objective of the present study was to construct a Caco-2 subline with reduced P-gp …
Conversion of the Ca2+-ATPase from Rhodospirillum rubrum into a Mg2+-dependent enzyme by 1,N6-etheno ATP
1980
Nucleoside triphosphate hydrolysis of R.rubrum ATPase complexes can be changed from Ca2+-dependence to Mg2+-dependence by replacing ATP with 1,N6-etheno ATP. Four ATPase complexes which have been prepared by different procedures hydrolyze ATP and 1,N6-etheno ATP at different rates in dependence on the added metal ions. These differences allow an easy distinction of the various enzyme forms.
Die Herstellung einer molekulareinheitlichen Tri-, Tetra- und Pentamethacrylsäure. V. Mitt. Modelle für Matrizenreaktionen
1968
Vom p-Kresol sowie einer phenolischen Zwei- und einer Dreikernverbindung wurden mit verschiedenen Acylierungsmethoden Methacrylsaureester erhalten; die Zweikernverbindung enthielt danach zwei und die Dreikernverbindung drei Methacrylreste. Loste man diese Methacrylsuureester in groser Verdunnung in siedendem Benzol und lies gleichzeitig einen grosen Uberschus an Radikalen aus α.α′-Azoisobuttersauredinitril einwirken, so wurde beim Methacrylsaure-p-kresylester eine Polymerisation unterdruckt. Bei den mehrfachen Estern der Mehrkernverbindungen wurde die polyrmerisationsahnliche Additionsreaktion auf die einzelnen Molekule begrenzt (intramolckulare Polymerisation); sie ergab neben einer cyclis…
Ein neuer weg zur herstellung von oligomeren aus acryl- und vinylverbindungen; modell einer matrizenreaktion
1966
In einer Reihe vorangehender Untersuchungen wurde die Herstellung molekulareinheitlicher, phenolischer Mehrkernverbindungen beschrieben. Sie besitzen je Phenolbaustein eine phenolische Hydroxylgruppe, wie systematisch untersuchte Acylierungen sowie Methylierung und Arylierung dieser Mehrkernverbindungen zeigen. In der vorliegenden Mitteilung wurde bei einer Zwei- und Dreikernverbindung an den phenolischen Hydroxylgruppen mit Acrylsaurechlorid umgesetzt, so das die entstandenen Verbindungen so viele polymerisationsfahige Reste wie phenolische Bausteine besasen. Die Polymerisation der Acrylsaurederivate in sehr groser Verdunnung gab oligomere Leiterverbindungen, die durch alkalische Hydrolyse…
Machine learning of reverse transcription signatures of variegated polymerases allows mapping and discrimination of methylated purines in limited tra…
2020
AbstractReverse transcription (RT) of RNA templates containing RNA modifications leads to synthesis of cDNA containing information on the modification in the form of misincorporation, arrest, or nucleotide skipping events. A compilation of such events from multiple cDNAs represents an RT-signature that is typical for a given modification, but, as we show here, depends also on the reverse transcriptase enzyme. A comparison of 13 different enzymes revealed a range of RT-signatures, with individual enzymes exhibiting average arrest rates between 20 and 75%, as well as average misincorporation rates between 30 and 75% in the read-through cDNA. Using RT-signatures from individual enzymes to trai…
CAF-like state in primary skin fibroblasts with constitutional BRCA1 epimutation sheds new light on tumor suppressor deficiency-related changes in he…
2016
Constitutive epimutations of tumor suppressor genes are increasingly considered as cancer predisposing factors equally to sequence mutations. In light of the emerging role of the microenvironment for cancer predisposition, initiation, and progression, we aimed to characterize the consequences of a BRCA1 epimutation in cells of mesenchymal origin. We performed a comprehensive molecular and cellular comparison of primary dermal fibroblasts taken from a monozygous twin pair discordant for recurrent cancers and BRCA1 epimutation, whose exceptional clinical case we previously reported in this journal. Comparative transcriptome analysis identified differential expression of extracellular matrix-r…