Search results for "ovine"

showing 10 items of 317 documents

Non-specific Hyperreactivity to Pharmacological Stimuli in Tracheal and Lung Parenchymal Strips of Actively Sensitized Guinea-pigs

1989

Abstract The responsiveness of tracheal and lung parenchymal strips isolated from actively sensitized guinea-pigs to CaCl2 (in K+-depolarized tissue), KCl, acetylcholine and histamine was compared with that of strips from unsensitized animals. The concentration-response curves to the mentioned agonists exhibited, in the sensitized group, a left upward displacement (greater maximal effect, lesser effective concentration 50% and a steeper slope) compared with those obtained in the unsensitized group. These results indicate the existence of a non-specific increase in responsiveness in the airway smooth muscle from sensitized animals.

Malemedicine.medical_specialtyGuinea PigsPharmaceutical ScienceIn Vitro TechniquesMuscle Smooth VascularPotassium ChlorideGuinea pigCalcium Chloridechemistry.chemical_compoundInternal medicineParenchymamedicineAnimalsRespiratory systemLungPharmacologyLungChemistrySerum Albumin BovineAcetylcholineTracheamedicine.anatomical_structureEndocrinologyAnesthesiaImmunohistochemistryHistamineAcetylcholineHistamineMuscle ContractionRespiratory tractmedicine.drugJournal of Pharmacy and Pharmacology
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Growth and safety evaluation of infant formulae containing oligosaccharides derived from bovine milk: a randomized, double-blind, noninferiority trial

2014

A limited number of nondigestible oligosaccharides are available for use in infant formula. This study evaluated growth and safety in infants fed formula supplemented with a mixture of bovine milk-derived oligosaccharides (BMOS). This mixture, which was generated from whey permeate, contains galactooligosaccharides and other oligosaccharides from bovine milk, such as 3′- and 6′-sialyllactose. We hypothesized that growth in infants fed BMOS-supplemented formula would be noninferior to that in infants fed standard formula. Healthy term infants ≤14 days old were randomly assigned to standard formula (control; n = 84); standard formula with BMOS (IF-BMOS; n = 99); or standard formula with BMOS …

Malemedicine.medical_specialtyPediatricsBifidobacterium longumOligosaccharidesGrowthWeight GainGastroenterologyInfant nutritionFecesChild DevelopmentLactobacillus rhamnosusDouble-Blind MethodInternal medicinemedicineAnimalsHumansPediatrics Perinatology and Child HealthGastrointestinal TransitFecesHealthy infantsbiologyAnthropometrybusiness.industryProbioticsInfant NewbornInfantbiology.organism_classificationConfidence intervalInfant FormulaMilkInfant formulaPediatrics Perinatology and Child HealthFood FortifiedVomitingCattleFemalemedicine.symptombusinessFlatulenceWeight gainBovine milk-derived oligosaccharidesResearch ArticleBMC Pediatrics
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Identification of calcium sensing receptor (CaSR) mRNA-expressing cells in normal and injured rat brain

2009

Calcium sensing receptor (CaSR), isolated for the first time from bovine and human parathyroid, is a G-protein-coupled receptors that has been involved in diverse physiological functions. At present a complete in vivo work on the identification of CaSR mRNA-expressing cells in the adult brain lacks and this investigation was undertaken in order to acquire more information on cell type expressing CaSR mRNA in the rat brain and to analyse for the first time its expression in different experimental models of brain injury. The expression of CaSR mRNAs was found mainly in scattered cells throughout almost all the brain regions. A double labeling analysis showed a colocalization of CaSR mRNA expr…

Malemedicine.medical_specialtyTime FactorsCentral nervous systemHippocampusCell CountSettore BIO/11 - Biologia MolecolareBiologySettore BIO/09 - Fisiologiachemistry.chemical_compoundSeizuresInternal medicineSettore BIO/10 - BiochimicaCaSRmedicineAnimalsRNA MessengerRats WistarIbotenic AcidMolecular BiologyIn Situ HybridizationNeuronsKainic AcidGeneral NeuroscienceDentate gyrusBrainColocalizationImmunohistochemistryRatsOligodendrogliamedicine.anatomical_structureEndocrinologynervous systemchemistryBrain InjuriesNeurogliaNeurology (clinical)Pyramidal cellCaSR; BrainCalcium sensing receptor (CaSR) isolated for the first time from bovine and human parathyroid is a G-protein-coupled receptors that has been involved in diverse physiological functions. At present a complete in vivo work on the identification of CaSR mRNA-expressing cells in the adult brain lacks and this investigation was undertaken in order to acquire more information on cell type expressing CaSR mRNA in the rat brain and to analyse for the first time its expression in different experimental models of brain injury. The expression of CaSR mRNAs was found mainly in scattered cells throughout almost all the brain regions. A double labeling analysis showed a colocalization of CaSR mRNA expression in neurons and oligodendrocytes whereas it was not found expressed both in the microglia and in astrocytes. One week after kainate-induced seizure CaSR was found in the injured CA3 region of the hippocampus and very interestingly it was found up-regulated in the neurons of CA1-CA2 and dentate gyrus. Similarly 1 week following ibotenic acid injection in the hippocampus CaSR mRNA expression was increased in oligodendrocytes both in the lesioned area and in the contralateral CA1-CA3 pyramidal cell layers and dentate gyrus. One week after needle-induced mechanical lesion an increase of labeled cells expressing CaSR mRNA was observed along the needle track. In conclusion the present results contribute to extend available data on cell type-expressing CaSR in normal and injured brain and could spur to understand the role of CaSR in repairing processes of brain injury.Receptors Calcium-SensingIbotenic acidDevelopmental BiologyAstrocyte
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Construction and evaluation of sponge scaffolds from hyaluronic acid derivatives for potential cartilage regeneration

2020

A two or one pot synthesis has been used for the reaction of hyaluronic acid (HA) with octadecylamine (C-18) and hydrazine (Hy). In both cases, the chemical derivatization involved primary hydroxyl groups of hyaluronic acid and not its carboxyl groups, whose presence is important for receptor interaction. In this way, Hy-HA-C-18 derivatives have been obtained with appropriate hydrophobic and hydrophilic character. Their ability to form homogeneous physical hydrogels has been evaluated as well as the possibility to obtain porous sponges through salt leaching technology. Sponges showing the highest porosity, potentially compatible with cell entrapment, have been characterized with regard to t…

Materials scienceBiomedical EngineeringSettore MED/08 - Anatomia PatologicaGlycosaminoglycanchemistry.chemical_compoundPhysiological conditionHyaluronidaseChemical derivatizationHyaluronic acidmedicineOrganic chemistryGeneral Materials ScienceHydrophobic and hydrophilicDerivatizationbiologyCartilageBovine chondrocyteGeneral ChemistryGeneral Medicinebiology.organism_classificationHyaluronic acid derivativeReceptor interactionSpongemedicine.anatomical_structurechemistryCartilage regenerationSettore CHIM/09 - Farmaceutico Tecnologico ApplicativoSelf-healing hydrogelsBiological propertieSwellingmedicine.symptommedicine.drugNuclear chemistryJournal of Materials Chemistry B
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Effect of different bleaching protocols on whitening efficiency and enamel superficial microhardness

2018

Background Tooth bleaching is a popular aesthetic treatment to modify the color of teeth. Despite the extensive literature concerning the subject, there is still no consensus regarding the application mode of the different bleaching agents and their effect on enamel. Therefore, this study evaluated the influence of different bleaching protocols on whitening efficiency and enamel superficial hardness. Material and methods Bovine enamel fragments were embedded in acrylic resin and wet-sanded to obtain a flat buccal surface. The specimens were then randomly divided into 6 groups (n=10), based on the bleaching material [HP Maxx 35% (35% hydrogen peroxide), HP Blue 35% (35% hydrogen peroxide + C…

Materials scienceEnamel paintgenetic structuresResearch030206 dentistryEsthetic Dentistry:CIENCIAS MÉDICAS [UNESCO]Indentation hardnessHardness03 medical and health scienceschemistry.chemical_compound0302 clinical medicinechemistrystomatognathic system030220 oncology & carcinogenesisvisual_artVickers hardness testUNESCO::CIENCIAS MÉDICASvisual_art.visual_art_mediumBovine enamelCarbamide peroxideHydrogen peroxideGeneral DentistryAcrylic resinNuclear chemistry
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Tuning protein adsorption on graphene surfaces via laser-induced oxidation

2021

An approach for controlled protein immobilization on laser-induced two-photon (2P) oxidation patterned graphene oxide (GO) surfaces is described. Selected proteins, horseradish peroxidase (HRP) and biotinylated bovine serum albumin (b-BSA) were successfully immobilized on oxidized graphene surfaces, via non-covalent interactions, by immersion of graphene-coated microchips in the protein solution. The effects of laser pulse energy, irradiation time, protein concentration and duration of incubation on the topography of immobilized proteins and consequent defects upon the lattice of graphene were systemically studied by atomic force microscopy (AFM) and Raman spectroscopy. AFM and fluorescence…

Materials scienceOxideBioengineering02 engineering and technology010402 general chemistry01 natural sciencesHorseradish peroxidaselaw.inventionsymbols.namesakechemistry.chemical_compoundlawFluorescence microscopeGeneral Materials ScienceBovine serum albuminbiologyGrapheneGeneral EngineeringGeneral Chemistry021001 nanoscience & nanotechnologyAtomic and Molecular Physics and Optics0104 chemical sciencesChemical engineeringchemistryBiotinylationbiology.proteinsymbols0210 nano-technologyRaman spectroscopyProtein adsorptionNanoscale Advances
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Multifunctional clickable and protein-repellent magnetic silica nanoparticles

2016

Silica nanoparticles are versatile materials whose physicochemical surface properties can be precisely adjusted. Because it is possible to combine several functionalities in a single carrier, silica-based materials are excellent candidates for biomedical applications. However, the functionality of the nanoparticles can get lost upon exposure to biological media due to uncontrolled biomolecule adsorption. Therefore, it is important to develop strategies that reduce non-specific protein-particle interactions without losing the introduced surface functionality. Herein, organosilane chemistry is employed to produce magnetic silica nanoparticles bearing differing amounts of amino and alkene func…

Materials scienceSurface PropertiesSilicon dioxideNanoparticleNanotechnology02 engineering and technology010402 general chemistry01 natural sciencesMagneticschemistry.chemical_compoundAdsorptionDynamic light scatteringAnimalsGeneral Materials Sciencechemistry.chemical_classificationBiomoleculeSerum Albumin BovineSilicon Dioxide021001 nanoscience & nanotechnologyDynamic Light ScatteringFerrosoferric Oxide0104 chemical sciencesElectrophoresischemistryCovalent bondThermogravimetryNanoparticlesPolystyrenesCattleElectrophoresis Polyacrylamide GelMuramidaseAdsorption0210 nano-technologyProtein adsorptionNanoscale
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Anomalous water dynamics in brain: a combined diffusion magnetic resonance imaging and neutron scattering investigation

2019

International audience; Water diffusion is an optimal tool for investigating the architecture of brain tissue on which modern medical diagnostic imaging techniques rely. However, intrinsic tissue heterogeneity causes systematic deviations from pure free-water diffusion behaviour. To date, numerous theoretical and empirical approaches have been proposed to explain the non-Gaussian profile of this process. The aim of this work is to shed light on the physics piloting water diffusion in brain tissue at the micrometre-to-atomic scale. Combined diffusion magnetic resonance imaging and first pioneering neutron scattering experiments on bovine brain tissue have been performed in order to probe dif…

Medical diagnosticMaterials science[SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/ImagingQuantitative Biology::Tissues and OrgansPhysics::Medical PhysicsBiomedical EngineeringBiophysicsproton dynamicsBioengineeringbrain imagingNeutron scatteringBiochemistryAtomic unitsBiomaterials03 medical and health sciences0302 clinical medicineTissue heterogeneityWater dynamicsNuclear magnetic resonancemedicineAnimalsDiffusion (business)030304 developmental biologydiffusion magnetic resonance imaging0303 health sciencesProton dynamicmedicine.diagnostic_testneutron scatteringBrainWaterMagnetic resonance imagingwater diffusionLife Sciences–Physics interfaceMagnetic Resonance ImagingSettore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)Neutron Diffraction[SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/ImagingBovine brainBrain imaging; Diffusion magnetic resonance imaging; Neutron scattering; Proton dynamics; Water diffusionCattle030217 neurology & neurosurgeryBiotechnology
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Role of membrane dynamics processes and exogenous molecules in cellular resveratrol uptake: consequences in bioavailability and activities.

2011

In the fields of nutrition prevention and therapy treatment, numerous studies have reported interesting properties of trans-resveratrol (RSV), a natural polyphenol against pathologies such as vascular diseases, cancers, viral infections and neurodegenerative processes. These beneficial effects are supported by more studies showing the pleiotropic actions of RSV. Nevertheless, a crucial question concerning these effects is how the polyphenol, when applied to an organism, gains access to its targets. In this review, we focus on the biochemical and biological parameters involved in RSV transport, particularly the role of the phospholipid bilayer in RSV uptake (passive diffusion, carrier-mediat…

Membrane FluidityvirusesLipoproteinsIntegrinEstrogen receptorBiological AvailabilityResveratrolEndocytosischemistry.chemical_compoundMembrane LipidsMembrane MicrodomainsCell surface receptorStilbenesAnimalsHumansReceptorLipid raftbiologyCell MembraneFatty Acidsvirus diseasesBiological TransportSerum Albumin Bovinerespiratory systemIntegrin alphaVbeta3EndocytosisCell biologyBiochemistrychemistryResveratrolbiology.proteinIntracellularFood ScienceBiotechnologyMolecular nutritionfood research
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ChemInform Abstract: Synthesis, Benzodiazepine Receptor Binding and Molecular Modelling of Isochromeno[4,3-c]pyrazol-5(1H)-one Derivatives.

2012

A series of isochromeno[4,3-c]pyrazol-5(1H)-one derivatives (III) is prepared and tested for their ability to displace specific [3H]flunitrazepam from bovine brain membranes.

MembraneBovine brainChemistryStereochemistrymedicineGeneral MedicineFlunitrazepamBenzodiazepine receptor bindingmedicine.drugChemInform
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