Search results for "pero"

Inhibition of the system luminol-H2O2-Fe(CN)63− chemiluminescence by the Mn(II) indirect determination of isoniazid in a pharmaceutical formulation

1998

A flow injection procedure for the indirect chemiluminescent determination of isoniazid is proposed. The method is performed in a flow-injection manifold provided with a solid-phase reactor. The reactor was made from manganese dioxide physically entrapped by polymerization; the redox reaction isoniazid-manganese dioxide released Mn(II) which was monitored through its inhibitory effect on the reaction between luminol and hydrogen peroxide in presence of potassium hexacyanoferrate (III). The procedure resulted in a linear calibration graph over the range 5-15 mg/L of isoniazid with a sample throughput of 43 samples/h. The influence of foreign compounds was studied and the method was applied t…

Determination of heavy metal content and lipid profiles in mussel extracts from two sites on the moroccan atlantic coast and evaluation of their biol…

2012

Mussels may concentrate pollutants, with possibly significant side effects on human health. Therefore, mussels (Mytilus galloprovincialis) from two sites of the Moroccan Atlantic coast (Jorf Lasfar [JL], an industrial site, and Oualidia [OL], a vegetable-growing area), were subjected to biochemical analyses to quantify the presence of heavy metals (Cd, Cr, and Pb) and to establish the lipid profile: fatty acid, cholesterol, oxysterol, phytosterol and phospholipid content. In addition, mussel lipid extracts known to accumulate numerous toxic components were tested on murine pancreatic β-cells (MIN6), and their biological activities were measured with various flow cytometric and biochemical m…

Endoplasmic reticulum‐resident chaperones modulate the inflammatory and angiogenic responses of endothelial cells

2015

SummaryBackground Wound healing depends on a well-balanced regulation of inflammation and angiogenesis. In chronic wounds the healing process is disturbed and inflammation persists. Regulation of wound closure is controlled by transmembrane and extracellular proteins, the folding and maturation of which occur in the endoplasmic reticulum (ER) by ER-resident chaperone machinery. Objectives To study the role of the ER-resident chaperones BiP/Grp78, its cochaperone Mdg1/ERdJ4, and Grp94 in chronic, nonhealing wounds. Methods Immunohistochemical staining of these chaperones in individual human biopsies and investigation of the possible role of BiP and Mdg1 in endothelial cells, focusing on thei…

Representation of Peronist unionism in Argentinian sociologist Roberto Carri’s works: From the “vandorismo” to the guerilla “Montoneros” (1967-1974)

2019

En este trabajo abordamos un aspecto de la trayectoria intelectual del sociólogo argentino Roberto Carri, referido a sus estudios sobre el sindicalismo argentino, específicamente el sindicalismo peronista después de 1955. Para hacerlo tomamos en cuenta tres textos claves que le dedicó al tema, en tres momentos particulares de su trayectoria intelectual y militante: el libro Sindicatos y Poder en la Argentina (1967), el artículo ?Sindicalismo de participación, sindicalismo de liberación? (1971), y el texto ?Vandorismo. La política del imperialismo para los trabajadores peronistas? (1974). En estos textos analizaremos cinco elementos: la lectura de la relación entre el peronismo y los sindica…

Secondary structure conformation of hydroperoxide lyase from green bell pepper, cloned in Yarrowia lipolytica, and its activity in selected media

2008

International audience; Circular dichroism (CD) spectroscopy of secondary structure conformation of the purified green bell pepper hydroperoxide lyase (HPL), cloned in the yeast Yarrowia lipolytica, was investigated. The CD spectra of HPL in iso-octane, obtained at 60 °C, in the presence of the reducing agent dithiothreitol showed dramatic increase in α-helix content. The enzyme conformation remained unchanged over a range of pH values of 5.0–7.0. Using 13-hydroperoxide of linoleic acid (13-HPOD) as substrate, the biocatalysis of HPL in organic solvent media, including chloroform, dichloromethane, hexane, iso-octane, octane and toluene, was investigated. The results indicated an increase in…

Citrate-nitrate auto-combustion synthesis of perovskite-type nanopowders: A systematic approach

2009

Edilizia abusiva a Favara. Analisi dell’insediamento e sviluppo del territorio

2007

Il Piano Regolatore Generale, strumento di fondamentale importanza per lo sviluppo di un centro urbano e del suo territorio, è ancora per diverse città siciliane un obiettivo da raggiungere. I dati provenienti dall'Assessorato Regionale al Territorio e Ambiente (A.R.T.A.) indicano una Sicilia decisamente indietro nella progettazione urbanistica. Nell'isola soltanto il 40,3% dei comuni hanno un piano regolatore vigente. La mancata approvazione degli strumenti urbanistici ha influito negativamente sui processi di urbanizzazione, spesso frammentari e disorganici. Le città si sono sviluppate senza una forma, un modello riconoscibile, alimentando nei cittadini una politica che ha preferito l’edi…

SAR-studies of γ-secretase modulators with PPARγ-agonistic and 5-lipoxygenase-inhibitory activity for Alzheimer’s disease

2014

Abstract We present the design, synthesis and biological evaluation of compounds containing a 2-(benzylidene)hexanoic acid scaffold as multi-target directed γ-secretase-modulators. Broad structural variations were undertaken to elucidate the structure–activity-relationships at the 5-position of the aromatic core. Compound 13 showed the most potent activity profile with IC50 values of 0.79 μM (Aβ42), 0.3 μM (5-lipoxygenase) and an EC50 value of 4.64 μM for PPARγ-activation. This derivative is the first compound exhibiting low micromolar to nanomolar activities for these three targets. Combining γ-secretase-modulation, PPARγ-agonism and inhibition of 5-lipoxygenase in one compound could be a …

Hsp60 molecular anatomy and role in colorectal cancer diagnosis and treatment

2011

Quantitative changes in Hsp60 during the development of some tumors suggest that this chaperonin plays a role in carcinogenesis. A description of the specific role(s) of Hsp60 in tumor-cell growth and proliferation is still incomplete, but it is already evident that monitoring its levels and distribution in tissues and fluids has potential for diagnosis and staging, and for assessing prognosis and response to treatment. Although Hsp60 is considered an intramitochondrial protein, it has been demonstrated in the cytosol, cell membrane, vesicles, cell surface, extracellular space, and blood. The knowledge that Hsp60 occurs at all these locations opens new avenues for basic and applied research…

Mitochondrial compartment: a possible target of cadmium effects on breast epithelial cells.

2009

Cadmium–breast epithelial cell interactions were studied by analyzing some mitochondria-related aspects of stress response. We treated immortalized non-tumor breast cells with 5 or 50 μM CdCl2 for 24 or 96 h demonstrating that the exposure did not cause a significant mitochondrial proliferation, while it induced a significant increase in the respiratory activity and mitochondrial polarization. In addition, we found that hsp60 was up-regulated while hsp70 and COXII and COXIV were down-regulated. The mRNA for hsp70 remained constant and only the inducible form of the 70-kDa heat shock protein was over expressed. The mRNAs for COXII and COXIV remained constant after 24 h and increased after lo…