Search results for "pharmacognosy"

showing 10 items of 127 documents

New acetophenone glucosides isolated from extracts of Helichrysum italicum with antiinflammatory activity.

2001

Three new acetophenone glucosides (4-6), three known aglycons (1-3), and a benzo-gamma-pyrone glucoside (7) were isolated from the CH(2)Cl(2), EtOAc, and BuOH extracts from the aerial parts of Helichrysum italicum. All the compounds tested showed antiinflammatory activity in a 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced mouse ear edema test, and the ID(50) value of compound 2, the most active compound, was determined.

KetoneMagnetic Resonance SpectroscopySpectrophotometry InfraredStereochemistryIndomethacinPharmaceutical SciencePharmacognosyAsteraceaeHelichrysum italicumAnalytical Chemistrychemistry.chemical_compoundMiceGlucosideGlucosidesPhenolsDrug DiscoverySpectroscopy Fourier Transform InfraredAnimalsEdemaBenzopyransPharmacologychemistry.chemical_classificationPlants MedicinalbiologyDose-Response Relationship DrugPlant Extractsbeta-GlucosidaseOrganic ChemistryAnti-Inflammatory Agents Non-SteroidalGlycosideAcetophenonesbiology.organism_classificationComplementary and alternative medicinechemistryAldoseActive compoundSpainMolecular MedicineTetradecanoylphorbol AcetateSpectrophotometry UltravioletChromatography Thin LayerAcetophenoneJournal of natural products
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Phenolic Substances from Phagnalon rupestre Protect against 2,4,6-Trinitrochlorobenzene-Induced Contact Hypersensitivity

2011

2-isoprenylhydroquinone-1-glucoside (1), 3,5-dicaffeoylquinic acid (2), and 3,5-dicaffeoylquinic acid methyl ester (3), isolated from Phagnalon rupestre, improved the contact hypersensitivity response to 2,4,6-trinitrochlorobenzene in mice. These phenolics reduced ear swelling and IL-1β content by 50% 24 h after challenge; in addition, 2 inhibited tumor necrosis factor-α by 53%. All three compounds also reduced interleukin-2 content by 50% 72 h after challenge. Both 2 and 3 inhibited metalloproteinase-9 levels in the skin lesions by 66% and 41%, respectively, and lowered cyclooxygenase-2 expression by 44% and 49%, respectively, at 24 h. Moreover, 2 was effective against atopic dermatitis in…

LipopolysaccharidesInterleukin-1betaPharmaceutical SciencePicryl ChloridePharmacognosyDermatitis ContactAnalytical ChemistryMicechemistry.chemical_compoundPhenolsDrug DiscoverymedicineAnimalsEdemaPhenolsSkinPharmacologychemistry.chemical_classificationCyclooxygenase 2 InhibitorsDose-Response Relationship DrugTraditional medicineTumor Necrosis Factor-alphaMacrophagesOrganic ChemistryContact hypersensitivityGlycosidePhagnalon rupestreEarmedicine.diseaseDisease Models AnimalComplementary and alternative medicineBiochemistrychemistryInterleukin-2Molecular MedicineContact dermatitisJournal of Natural Products
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Effects of Scrophularia ningpoensis Hemsl. on Inhibition of Proliferation, Apoptosis Induction and NF-κB Signaling of Immortalized and Cancer Cell Li…

2012

Scrophularia ningpoensis has been used in China for centuries as a herbal tea to treat various diseases. Based on the numerous animal studies on its pharmaceutical effects and the long time clinical experiences, we studied the molecular and cellular mechanism underlying the bioactivity of aqueous extract of Scrophularia and its isolated compounds. Seven isolated compounds, unlike Scrophularia extract, failed to induce cytotoxicity on HaCaT cells, but their combination improved the effect of extract. Tumor cell line selectivity was not observed, when we studied its cytotoxic effect on melanoma cell lines. The apoptotic and anti-inflammatory effects of Scrophularia extract have been demonstra…

MAPK/ERK pathwaypharmacognosylcsh:Medicinelcsh:RS1-441Pharmaceutical SciencePharmacologyapoptosis; microarrays; pharmacogenomics; pharmacognosy; phytotherapy; signaling pathwaysArticlelcsh:Pharmacy and materia medicaDrug DiscoveryMedicineScrophulariaCytotoxicitymicroarrayspharmacogenomicsScrophularia ningpoensisbiologybusiness.industrylcsh:Rapoptosisphytotherapybiology.organism_classificationsignaling pathwaysHaCaTMechanism of actionApoptosisMolecular MedicineSignal transductionmedicine.symptombusinessPharmaceuticals
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3-Oxo-rhazinilam:  A New Indole Alkaloid from Rauvolfia serpentina × Rhazya stricta Hybrid Plant Cell Cultures

2000

A new monoterpenoid indole alkaloid, 3-oxo-rhazinilam (1), was isolated from intergeneric somatic hybrid cell cultures of Rauvolfia serpentina and Rhazya stricta, and the structure was determined by detailed 1D and 2D NMR analysis. It was also proved that 3-oxo-rhazinilam (1) is a natural constituent of the hybrid cells.

Magnetic Resonance SpectroscopyLactamsPharmaceutical SciencePharmacognosyRhazya strictaRhazinilamMass SpectrometryRauwolfiaAnalytical Chemistrychemistry.chemical_compoundAlkaloidsRauvolfia serpentinaDrug DiscoveryBotanyCells CulturedPharmacologyPlants MedicinalIndole alkaloidbiologyApocynaceaeOrganic ChemistryIndolizinesbiology.organism_classificationCoculture TechniquesTerpenoidSomatic fusionComplementary and alternative medicinechemistryMolecular MedicineSpectrophotometry UltravioletJournal of Natural Products
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Anti-Inflammatory Triterpenes from Pistacia terebinthus Galls

2002

From the galls of Pistacia terebinthus we obtained an extract that proved to be effective against chronic and acute inflammation. Now we report on the isolation and identification of three triterpenes: two tirucallane-type lanostanoids and one oleanane, which we have identified as masticadienonic acid (1), masticadienolic acid (2), and morolic acid (3), respectively. All of them showed effectiveness on the mouse ear inflammation induced by repeated applications of 12-O-tetradecanoylphorbol 13-acetate and on the phospholipase A2-induced foot paw edema. The pharmacological activity of the compounds was ratified by a histological study of the ear samples. In addition, they inhibited leukotrien…

Magnetic Resonance SpectroscopyNeutrophilsmedicine.drug_classLeukotriene B4Anti-Inflammatory AgentsCyproheptadinePharmaceutical SciencePharmacologyPharmacognosyLeukotriene B4Pistacia terebinthusDexamethasonePhospholipases AAnti-inflammatoryAnalytical ChemistryMicechemistry.chemical_compoundPhospholipase A2TriterpeneDrug DiscoverymedicineAnimalsOleananeCalcimycinInflammationPharmacologychemistry.chemical_classificationDose-Response Relationship DrugIonophoresbiologyPistaciaPlant ExtractsOrganic Chemistrybiology.organism_classificationTriterpenesHindlimbPhospholipases A2Complementary and alternative medicinechemistryBiochemistryPistaciabiology.proteinTetradecanoylphorbol AcetateMolecular MedicinePlanta Medica
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Two new biologically active triterpene saponins from Acanthophyllum squarrosum.

2000

Two novel triterpenoid saponins (1 and 2) have been isolated from the roots of Acanthophyllum squarrosum. The structures were established mainly by a combination of 2D NMR techniques as 3-O-beta-D-galactopyranosyl-(1-->2)-[beta-D-xylopyranosyl-(1-->3)]-be ta-D-glucuronopyranosylgypsogenin-28-O-beta-D-xylopyranosyl-(1-->3 )-b eta-D-xylopyranosyl-(1-->4)-alpha-L-rhamnopyranosyl-(1-->4)-[alpha-L- rhamnopyranosyl-(1-->3)]-beta-D-fucopyranoside (1) and 3-O-beta-D-glucopyranosylgypsogenin-28-O-alpha-L-rhamnopyranosyl-( 1-- >2)-alpha-L-arabinopyranosyl-(1-->2)-[beta-D-glucopyranosyl-(1-->6 )]- beta-D-glucopyranoside (2). Compound 1 showed a moderate concentration-dependent immunomodulatory effect …

Magnetic Resonance SpectroscopySpectrophotometry InfraredStereochemistryChemical structureMolecular Sequence DataSaponinPharmaceutical SciencePharmacognosySpectrometry Mass Fast Atom BombardmentPlant RootsAnalytical Chemistrychemistry.chemical_compoundTriterpeneAdjuvants ImmunologicDrug DiscoveryLymphocytesOleanolic AcidOleanolic acidPharmacologychemistry.chemical_classificationPlants MedicinalPlant ExtractsHydrolysisOrganic ChemistryGlycosideNuclear magnetic resonance spectroscopySaponinsTerpenoidComplementary and alternative medicinechemistryCarbohydrate SequenceMolecular MedicineCell DivisionJournal of natural products
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A new biologically active acylated triterpene saponin from Silene fortunei.

1998

A new acylated triterpene-saponin (1), together with a mixture of the known jenisseensosides C and D, has been isolated from the roots of Silene fortunei. The structure of the new compound was established by chemical means and spectroscopic methods as 3-O-[beta-D-galactopyranosyl-(1-->2)-beta-D-glucuronopyranosyl]-28 -O- [[alpha-L-arabinopyranosyl-(1-->2)-alpha-L-arabinopyranosyl- (1-->3)-b eta-D-xylopyranosyl-(1-->4)-alpha-L-rhamnopyranosyl-(1-->2)]-[beta-D- glucopyranosyl-(1-->3)]-4-O-acetyl-beta-D-fucopyranosyl]quillaic acid. This saponin showed a significant enhancement of granulocyte phagocytosis in vitro.

Magnetic Resonance SpectroscopyStereochemistryChemical structureT-LymphocytesMolecular Sequence DataSaponinPharmaceutical ScienceUronic acidPharmacognosySpectrometry Mass Fast Atom BombardmentLymphocyte ActivationAnalytical Chemistrychemistry.chemical_compoundTriterpenePhagocytosisDrug DiscoveryCarbohydrate ConformationOleanolic AcidPharmacologychemistry.chemical_classificationSilenePlants MedicinalbiologyChemistryOrganic ChemistryGlycosideSaponinsbiology.organism_classificationTerpenoidTriterpenesComplementary and alternative medicineCarbohydrate SequenceMolecular MedicineGranulocytesJournal of natural products
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Isolation and Structure Elucidation of a New Indole Alkaloid from Rauvolfia serpentina Hairy Root Culture: The First Naturally Occurring Alkaloid of …

2002

A new monoterpenoid indole alkaloid, 10-hydroxy- N(alpha)-demethyl-19,20-dehydroraumacline ( 1), was isolated as a mixture of E- and Z-isomers from hairy root culture of Rauvolfia serpentina Benth. ex Kurz (Apocynaceae) and the structure was determined by 1D and 2D NMR analyses. The new indole alkaloid represents the first naturally occurring alkaloid of the raumacline group and its putative biosynthetical pathway is discussed.

Magnetic Resonance SpectroscopyStereochemistryMonoterpenePharmaceutical SciencePharmacognosyPlant RootsRauwolfiaIndole AlkaloidsAnalytical ChemistryRauvolfia serpentinaDrug DiscoveryBotanyCells CulturedPharmacologyCarbon IsotopesMolecular StructurebiologyApocynaceaeIndole alkaloidPlant ExtractsAlkaloidOrganic ChemistryStereoisomerismbiology.organism_classificationSecologanin Tryptamine AlkaloidsTerpenoidComplementary and alternative medicineHairy root cultureMolecular MedicinePlanta Medica
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Triterpene Saponins from Tupidanthus calyptratus

2001

Five new bisdesmosidic saponins (1--5) were isolated from the aerial parts of Tupidanthus calyptratus. Their structures were determined by (1)H--(1)H correlation spectroscopy (COSY, TOCSY, ROESY) and (1)H--(13)C correlation (HSQC, HMBC) NMR experiments, FABMS, and chemical data.

Magnetic Resonance SpectroscopyStereochemistrySaponinOligosaccharidesPharmaceutical ScienceSpectrometry Mass Fast Atom BombardmentPharmacognosyAnalytical ChemistryTriterpeneDrug DiscoveryHumansPharmacologychemistry.chemical_classificationPlants MedicinalChemistryOrganic ChemistryGlycosideSaponinsAntineoplastic Agents PhytogenicTriterpenesTerpenoidXylosideItalyComplementary and alternative medicineTriterpene SaponinsArialiaceae plantsMolecular MedicineDrug Screening Assays AntitumorTwo-dimensional nuclear magnetic resonance spectroscopyHeteronuclear single quantum coherence spectroscopy
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Bidentatoside I, a New Triterpene Saponin from Achyranthes bidentata

2001

Bidentatoside I (1) is a new triterpene saponin bearing an unusual dioxopropionic acid unit, isolated from the roots of Achyranthes bidentata. Structural elucidation was performed mainly by chemical and homo- and heteronuclear 2D NMR techniques. This compound did not show any potentiation of the in vitro cytotoxicity of cisplatin in the HT 29 human colon cancer cell line.

Magnetic Resonance SpectroscopyStereochemistrySaponinPharmaceutical ScienceAntineoplastic AgentsUronic acidPharmacognosyAnalytical ChemistryMagnoliopsidachemistry.chemical_compoundTriterpeneDrug DiscoveryTumor Cells CulturedHumansAchyranthes bidentataPharmacologychemistry.chemical_classificationbiologyOrganic ChemistryGlycosideSaponinsbiology.organism_classificationTriterpenesTerpenoidModels ChemicalComplementary and alternative medicinechemistryBiochemistryHeteronuclear moleculeColonic NeoplasmsMolecular MedicineDrug Screening Assays AntitumorJournal of Natural Products
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