Search results for "physiologic"
showing 10 items of 2593 documents
TrpM, a Small Protein Modulating Tryptophan Biosynthesis and Morpho-Physiological Differentiation in Streptomyces coelicolor A3(2).
2016
In the model actinomycete Streptomyces coelicolor A3(2), small open reading frames encoding proteins with unknown functions were identified in several amino acid biosynthetic gene operons, such as SCO2038 (trpX) in the tryptophan trpCXBA locus. In this study, the role of the corresponding protein in tryptophan biosynthesis was investigated by combining phenotypic and molecular analyses. The 2038KO mutant strain was characterized by delayed growth, smaller aerial hyphae and reduced production of spores and actinorhodin antibiotic, with respect to the WT strain. The capability of this mutant to grow on minimal medium was rescued by tryptophan and tryptophan precursor (serine and/or indole) su…
Stuck at work? Quantitative proteomics of environmental wine yeast strains reveals the natural mechanism of overcoming stuck fermentation
2015
During fermentation oenological yeast cells are subjected to a number of different stress conditions and must respond rapidly to the continuously changing environment of this harsh ecological niche. In this study we gained more insights into the cell adaptation mechanisms by linking proteome monitoring with knowledge on physiological behaviour of different strains during fermentation under model winemaking conditions. We used 2D-DIGE technology to monitor the proteome evolution of two newly discovered environmental yeast strains Saccharomyces bayanus and triple hybrid Saccharomyces cerevisiae × Saccharomyces kudriavzevii × S. bayanus and compared them to data obtained for the commercially a…
The skeleton of the staghorn coral Acropora millepora: molecular and structural characterization.
2014
15 pages; International audience; The scleractinian coral Acropora millepora is one of the most studied species from the Great Barrier Reef. This species has been used to understand evolutionary, immune and developmental processes in cnidarians. It has also been subject of several ecological studies in order to elucidate reef responses to environmental changes such as temperature rise and ocean acidification (OA). In these contexts, several nucleic acid resources were made available. When combined to a recent proteomic analysis of the coral skeletal organic matrix (SOM), they enabled the identification of several skeletal matrix proteins, making A. millepora into an emerging model for biomi…
Proteomics of CaCO3 biomineral-associated proteins: how to properly address their analysis.
2013
8 pages; International audience; In a recent editorial (Proc. Natl. Acad. Sci., 2013 110, E2144-E2146) and elsewhere, questions have been raised regarding the experimental practices in relation to the proteomic analysis of organic matrices associated to the biomineralized CaCO3 skeletons of metazoans such as molluscan shells and coral skeletons. Indeed, although the use of new high sensitivity MS technology potentially allows to identify a greater number of proteins, it is also equally (or even more) sensitive to contamination of residual proteins from soft tissues, which are in close contact with the biomineral. Based on our own past and present experimental know-how-observations that are …
Nacre calcification in the freshwater mussel Unio pictorum: carbonic anhydrase activity and purification of a 95 kDa calcium-binding glycoprotein.
2008
9 pages; International audience; The formation of the molluscan shell is finely tuned by macromolecules of the shell organic matrix. Previous results have shown that the acid-soluble fraction of the nacre matrix of the freshwater paleoheterodont bivalve Unio pictorum shell displays a number of remarkable properties, such as calcium-binding activity, the presence of extensive glycosylations and the capacity to interfere at low concentration with in vitro calcium carbonate precipitation. Here we have found that the nacre-soluble matrix exhibits a carbonic anhydrase activity, an important function in calcification processes. This matrix is composed of three main proteinaceous discrete fraction…
BAG3 Proteomic Signature under Proteostasis Stress
2020
The multifunctional HSP70 co-chaperone BAG3 (BCL-2-associated athanogene 3) represents a key player in the quality control of the cellular proteostasis network. In response to stress, BAG3 specifically targets aggregation-prone proteins to the perinuclear aggresome and promotes their degradation via BAG3-mediated selective macroautophagy. To adapt cellular homeostasis to stress, BAG3 modulates and functions in various cellular processes and signaling pathways. Noteworthy, dysfunction and deregulation of BAG3 and its pathway are pathophysiologically linked to myopathies, cancer, and neurodegenerative disorders. Here, we report a BAG3 proteomic signature under proteostasis stress. To elucidat…
The concept of major depression. I. Descriptive comparison of six competing operational definitions including ICD-10 and DSM-III-R.
1991
All operationalized diagnostic systems contain a diagnostic category, which corresponds to the concept of major depression. Yet, these corresponding definitions are not identical. Up to now, no comprehensive comparisons of the competing diagnoses have been published. We will therefore present a series of studies, describing six different operational definitions of major depression according to their content and construction and empirically comparing them in large inpatient and outpatient samples. This first paper presents a descriptive comparison of the definitions given in the Feighner Diagnostic Criteria, the Research Diagnostic Criteria, the Diagnostic and Statistical Manual of Mental Di…
Review of the “autobiographical space” in Boris Vian’s narrative
2019
This article addresses the difficulty of establi - shing limits and categories between reality and fiction in the narrative work and the “writing of the self” by Boris Vian, in relation to the prin - ciples of the Pataphysics College to which he belongs. For this, through the concept of “auto - biographical space” of Lejeune, which outlines and modifies his theory previously exposed in the Pacte autobiographique, we will focus on the techniques of the language-universe that plays with the principle of identity through the pseudonyms, the self-citation and the no - velistic transpositions of friends and acquain - tances who, masked, are participants in the vi - cissitudes of their fictional …
El exilio en Adieu, vive clarté... de Jorge Semprún: la superación de un trauma
2018
francaisL’exil est une experience avec des consequences traumatiques qui a accompagne l’etre humain pendant toute l’Histoire. Il s’agit d’un traumatisme caracterise par sa multiplicite et diversite de manifestations, il provoque une rupture ineffacable dans la vie du sujet. Sur le champ litteraire, il se montre comme un motif d’ecriture ou l’auteur cherche a comprendre et a donner du sens a son experience a travers la mise en discours. Cet article propose d’analyser l’experience de l’exil de l’ecrivain Jorge Semprun. Pour cela, on abordera, en premier lieu, la triple perte de la mere, l’enfance et la patrie; en deuxieme lieu, s’exposera l’entree forcee dans l’epoque adulte et; en dernier li…
Simultaneous and successive cognitive processes in brain damaged adults: Hemispheric and anterior-posterior effects
1988
The relationship between simultaneous and successive processing and their assumed underlying neuro-anatomical structures was examined. According to the model of Das, Kirby, and Jarman (1975, 1979) simultaneous processing, occurs mainly in the posterior parts of the brain (parieto-occipital areas) and successive processing in anterior regions of the brain (fronto-temporal areas). The theory of lateralized hemispheric specialization suggests differences in processing due to right-left hemispheric differences. A battery of measures was factor-analyzed and simultaneous and successive factors identified in 106 brain-damaged adults and a control group. The brain damaged group was divided into fou…