Search results for "plasmon"

Defined-size DNA triple crossover construct for molecular electronics: modification, positioning and conductance properties.

2011

We present a novel, defined-size, small and rigid DNA template, a so-called B-A-B complex, based on DNA triple crossover motifs (TX tiles), which can be utilized in molecular scale patterning for nanoelectronics, plasmonics and sensing applications. The feasibility of the designed construct is demonstrated by functionalizing the TX tiles with one biotin-triethylene glycol (TEG) and efficiently decorating them with streptavidin, and furthermore by positioning and anchoring single thiol-modified B-A-B complexes to certain locations on a chip via dielectrophoretic trapping. Finally, we characterize the conductance properties of the non-functionalized construct, first by measuring DC conductivi…

Protein-membrane interaction probed by single plasmonic nanoparticles.

2008

We present a nanosized and addressable sensor platform based on membrane coated plasmonic particles and show unequivocally the covering with lipid bilayers as well as the subsequent detection of streptavidin binding to biotinylated lipids. The binding is detected on membrane covered gold nanorods by monitoring the spectral shift by fast single particle spectroscopy (fastSPS) on many particles in parallel. Our approach allows for local analysis of protein interaction with biological membranes as a function of the lateral composition of phase separated membranes.

Reactive Surface Coatings Based on Polysilsesquioxanes: Controlled Functionalization for Specific Protein Immobilization

2009

The key designing in reliable biosensors is the preparation of thin films in which biomolecular functions may be immobilized and addressed in a controlled and reproducible manner. This requires the controlled preparation of specific binding sites on planar surfaces. Poly(methylsilsesquioxane)-poly(pentafluorophenyl acrylates) (PMSSQ-PFPA) are promising materials to produce stable and adherent thin reactive coatings on various substrates. Those reactive surface coatings could be applied onto various materials, for example, gold, polycarbonate (PC), poly(tetrafluoroethylene) (PTFE), and glass. By dipping those substrates in a solution of a desired amine, specific binding sites for protein ads…

Surface functionalization and surface recognition: Plasmon optical detection of molecular recognition at self assembled monolayers

1991

The synthesis of biotin- functionalized organic mercaptans and their chemisorption on gold surfaces is described. Biotin bound covalently to self assembled monolayers is recognized by streptavidin from aqueous buffer solutions. Spacer length and packing density of the biotin labels on the organic surface determine the docking kinetics. With a flexible and hydrophilic spacer very fast -diffusion controlled-docking is observed. As an alternative method of self assembly the spreading of organic mercaptans on water surfaces is established. Pressure-area diagrams of different functionalized mercaptans and disulfides are shown and their monolayer properties are discussed.

Specific Protein Binding to Functionalized Interfaces

1992

We report on the characterization of specific binding reactions between streptavidin and biotinylated model membrane surfaces. Self-assembly techniques as well as the Langmuir-Blodgett-Kuhn method were employed to prepare reactive, functionalized surfaces on various solid supports in contact with the aqueous protein solution. Plasmon surface polaritons optical measurements as well as atomic force microscopy and studies with the surface forces apparatus give rather detailed information as to the streptavidin monolayer formation, the kinetics of this process (either binding site- or diffusion limited), the selectivity of the reaction at laterally heterogeneous membranes, and the involved inte…

Protein-Membrane Interaction

2012

In the following chapter I present a nanosized and addressable protein sensor platform based on membrane coated plasmonic particles. I show unequivocally the covering of gold nanorods with lipid bilayers and thereafter the subsequent detection of streptavidin binding to biotin moities in the lipids. The binding of the streptavidin on the membrane covered gold nanorods is detected by monitoring the spectral shift by fast single particle spectroscopy (fastSPS) on many particles in parallel.

Quantum Nanoplasmonic : from dressed atom picture to superradiance

2019

Controlling quantum emitters (atoms, molecules, quantum dots, etc.), light, and its interactions is a key issue for implementing devices for information optical processing at the quantum level. For example, controlling dynamics of emitters coupled to a high-Q cavity can be achieved through cavity quantum electrodynamics (cQED). Plasmonic structures hybrid system are of growing interest in the quantum control at the nanoscale because of their capability to confine light beyond the diffraction limit. However, its application appears notoriously limited in practical situations due to the intrinsic presence of numerous and lossy modes, which complicates the description and the interpretation of…

Disclosing of optical properties of metal/dielectric heterostructures by Spectroscopic Ellipsometry enhanced by Surface Plasmon Resonance

2013

Absence of mutual polariton scatterings for strongly coupled surface plasmon polaritons and dye molecules with large Stokes shift

2013

The understanding and control of the dynamics of hybrid modes consisting of strongly coupled surface plasmon polaritons and molecular excitations of dye molecules is of great timely interest, as it allows one to tailor interactions between optical signals as needed for active all-optical devices. Here we utilize dye molecules with an especially large Stokes shift to demonstrate the absence of mutual scatterings among the strongly coupled hybrid modes. We employ a quantum mechanical three-level model and show that the hybrid modes decay via dephasing and internal relaxation of the molecules to a fluorescing state of the dye, which can be used as a measure for the decay. Our results provide e…

Plasmonic nanostructures through DNA-assisted lithography

2018

DALI combines DNA origami with conventional top-down fabrication for creating designer high-resolution plasmonic nanostructures.