Search results for "retrotransposon"

showing 5 items of 35 documents

Structural and evolutionary analysis of the copia-like elements in the Arabidopsis thaliana genome.

2001

The analysis of 460 kb of genomic sequence of Arabidopsis thaliana chromosome III allowed us to identify two new transposable elements named AtC1 and AtC2. AtC1 shows identical long terminal repeats (LTRs) and all the structural features characteristic of the copia-like active elements. AtC2 is also a full copia-like element, but a putative stop codon in the open reading frame (ORF) would produce a truncated protein. In order to identify the copia-like fraction of the A. thaliana genome, a careful computer-based analysis of the available sequences (which correspond to 92% of the genome) was performed. Approximately 300 nonredundant copia-like sequences homologous to AtC1 and AtC2 were detec…

Transposable elementDatabases FactualArabidopsisSequence HomologyRetrotransposonBiologyGenomeEvolution MolecularMagnoliopsidaOpen Reading FramesGeneticsArabidopsis thalianaAmino Acid SequenceMolecular BiologyEcology Evolution Behavior and SystematicsPhylogenyExpressed Sequence TagsPhylogenetic treeModels GeneticfungiTerminal Repeat SequencesSequence Analysis DNAModels Theoreticalbiology.organism_classificationStop codonLong terminal repeatOpen reading frameGenesEvolutionary biologyDNA Transposable ElementsSequence AlignmentGenome PlantSoftwareMolecular biology and evolution
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Tirant is a new member of the gypsy family of retrotransposons in Drosophila melanogaster.

2000

In this paper, we propose a consensus sequence for a putative complete Tirant retrotransposon. Several defective copies, as well as relevant sequences available in databases have been analyzed. The putative complete Tirant element is 8533 bp long, and presents all the structural features of a retroviruslike transposable element of the gypsy family. It contains three ORFs (open reading frames) that encode putative products resembling the retroviral Gag, Pol, and Env proteins. Southern blot analyses show that complete and defective Tirant elements are widespread in Drosophila melanogaster. The different hybridization patterns observed in several natural populations of this species suggest tha…

Transposable elementRetroelementsvirusesRetrotransposonGenes envOpen Reading FramesPhylogeneticsConsensus SequenceGeneticsConsensus sequenceAnimalsORFSMolecular BiologyPhylogenySouthern blotGeneticsbiologyBase SequenceTerminal Repeat SequencesGeneral Medicinebiology.organism_classificationGenes gagGenes polOpen reading frameDrosophila melanogasterDrosophila melanogasterSequence AlignmentBiotechnologyGenome
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bilbo, a non-LTR retrotransposon of Drosophila subobscura: a clue to the evolution of LINE-like elements in Drosophila

1997

We used the repetitive character of transposable elements to isolate a non-LTR retrotransposon in Drosophila subobscura. bilbo, as we have called it, has homology to TRIM and LOA elements. Sequence analysis showed a 5' untranslated region (UTR), an open reading frame (ORF) with no RNA-binding domains, a downstream ORF that had structural homology to that of the I factor, and, finally, a 3' UTR which ended in several 5-nt repeats. The results of our phylogenetic and structural analyses shed light on the evolution of Drosophila non-LTR retrotransposons and support the hypothesis that an ancestor of these elements was structurally complex.

Transposable elementUntranslated regionRetroelementsSequence analysisvirusesMolecular Sequence DataRetrotransposonBiologyPolymerase Chain ReactionHomology (biology)Evolution MolecularGeneticsAnimalsAmino Acid SequenceCloning MolecularMolecular BiologyPhylogenyEcology Evolution Behavior and SystematicsDNA PrimersRepetitive Sequences Nucleic AcidGeneticsBase SequenceSequence Homology Amino AcidPhylogenetic treeDrosophila subobscuraOpen reading frameDrosophilaMolecular Biology and Evolution
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Innovative and Applied Research in Biology: Proceedings, Vol. 3/2021

2021

The collection contains SCIENTIFIC articles on the topics of the LU 79 conference reports. The main focus is on innovative and applied research in biology and interdisciplinary fields.

iPBSBiofragmentationDrosophila melanogaster reproductive successProtecting organisms from UV-B radiation and extremely low-frequency electromagnetic fieldsBiotextile protection propertiesCloudberryGenetic diversitySoil burial testBacterial cellulose:NATURAL SCIENCES::Biology [Research Subject Categories]RetrotransposonsIsocitrate dehydrogenase as a candidate locusImmature gametic cellsAcetic acid bacteriaEcosystem servicesThe Daugava RiverTrifolium repensCypripedium calceolusScanning electron microscopy
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Retrotranspozonu struktūra parastās priedes (Pinus sylvestris L.) genomā un to ekspresija

2014

ANOTĀCIJA Promocijas darbs izstrādāts Latvijas Valsts mežzinātnes institūtā „Silava”. Pētījuma mērķis bija identificēt aktīvos retrotranspozonus parastās priedes (Pinus sylvestris L.) genomā un raksturot to transkripcijas līmeni dažādu stresa veidu ietekmē kontrolētos apstākļos. Pētījumā izmantoti nespecifiskie retrotranspozonu marķieri balstīti uz augsti konservatīvām tRNS piesaistes vietām. Viena klona priežu rametus pakļāva dabā izplatītam karstuma stresam, kukaiņu invāzijai (priežu hermess (Pineus pini L.), kā arī mākslīgi izraisītai augu hormonu (abscīzskābes un salicilskābes) ietekmei. Darba rezultātā identificēti pārsvarā I klases mobīlo elementu pārstāvji, kā arī tos saturošie himēr…

retrotranspozonidiferenciāla ekspresijagymnospermsMolecular biologyScots pine (Pinus sylvestris L.)kailsēkļiDzīvās dabas zinātnesBioloģijaTransposable elementsMolekulārā bioloģijaTransponējamie elementiretrotransposonsdifferential expression
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