Search results for "spread"
showing 10 items of 292 documents
Axial resolution in two-color excitation fluorescence microscopy by phase-only binary apodization
2005
We study the effect of a kind of binary phase-only filters, the Toraldo filters, in two-color excitation fluorescence microscopy. We show that by simple insertion of a properly designed Toraldo filter in one of the illumination arms the axial resolution of the system is significantly improved. Specifically, the main peak of the point spread function is narrowed by 22% along the axial direction.
Long working range light field microscope with fast scanning multifocal liquid crystal microlens array
2018
The light field microscope has the potential of recording the 3D information of biological specimens in real time with a conventional light source. To further extend the depth of field to broaden its applications, in this paper, we proposed a multifocal high-resistance liquid crystal microlens array instead of the fixed microlens array. The developed multifocal liquid crystal microlens array can provide high quality point spread function in multiple focal lengths. By adjusting the focal length of the liquid crystal microlens array sequentially, the total working range of the light field microscope can be much extended. Furthermore, in our proposed system, the intermediate image was placed i…
Sidelobe decline in single-photon 4Pi microscopy by Toraldo rings.
2003
We demonstrate theoretically the feasibility of single-photon 4Pi-confocal microscopy. By inserting a pair of properly designed multi-ring phase-only pupil filters in the illumination path of a 4Pi microscope the height of the sidelobes of the point spread function substantially reduced, so that there is no ambiguity in the 3D image. Then, an axial resolution up to four times higher than that of single-photon confocal microscope can be effectively achieved.
Observation of the cosmic ray moon shadowing effect with the ARGO-YBJ experiment
2011
Cosmic rays are hampered by the Moon and a deficit in its direction is expected (the so-called Moon shadow). The Moon shadow is an important tool to determine the performance of an air shower array. Indeed, the westward displacement of the shadow center, due to the bending effect of the geomagnetic field on the propagation of cosmic rays, allows the setting of the absolute rigidity scale of the primary particles inducing the showers recorded by the detector. In addition, the shape of the shadow permits to determine the detector point spread function, while the position of the deficit at high energies allows the evaluation of its absolute pointing accuracy. In this paper we present the obser…
Angular spreading measurements using MeV ion microscopes
2013
Abstract The sharpness of MeV ion microscope images is governed by small-angle scattering and associated lateral spreading of the ion beam in the sample. We have investigated measurement of the half-angle of the angular spreading distribution by characterising the image blurring in direct-Scanning Transmission Ion Microscopy (direct-STIM). In these tests Mylar™ foils of 0.5–6 μm were used to induce angular spreading. Images were taken of an electron microscope grid using 2 MeV protons with, and without, the foils in the beam path. The blurring was measured by fitting the width of a circular Gaussian point spread function to the images with and without the foil in position. The results show …
Manufacture of pupil filters for 3D beam shaping
2007
In a previous work we presented a new method for binarizing pupil filters designed to control the three-dimensional (3D) irradiance distribution in the focal volume of apodized systems. The method is based in the fact that the 3D amplitude point spread function of an axially-symmetrical system can be recovered entirely from a one-dimensional (1D) set of regularly spaced amplitude samples. Hence we proposed the use of 1D iterative Fourier transform algorithm to binarize a, properly mapped, version of the amplitude transmittance of the filter. The binary masks obtained consist of a set of opaque and transparent concentric annular zones. In this paper we have built two of these masks with oppo…
Ultracompact x-ray dosimeter based on scintillators coupled to a nano-optical antenna
2017
International audience; We show that nano-optical antennas are capable of controlling the luminescence induced by the absorption of x rays into matter. The x-ray-excited luminescence from a tiny scintillation cluster coupled to a horn nano-optical antenna is highly directed and determined by the antenna’s geometrical parameters. Directionality is sufficiently high to efficiently outcouple the x-ray-excited luminescence to a narrow single-mode optical fiber, thus enabling ultracompact fiber-integrated x-ray sensors. Our nano-optically driven approach offers the possibility of x-ray profiling and dosimetry in ultra-confined environments, opening up new avenues in the fields of x-ray imaging, …
Light Sheet Fluorescence Microscopy (LSFM) for Two-Photon Excitation Imaging of Thick Samples.
2015
Over the last decades, fluorescence microscopy techniques have been developed in order to provide a deeper, faster and higher resolution imaging of three-dimensional biological samples. Within this framework, Light Sheet Fluorescence Microscopy (LSFM) became an increasingly useful and popular imaging technique able to answer several biological questions in the field of developmental biology [1]. Thanks to the spatial confinement of the excitation process within a thin sheet in the focal plane, it provides an intrinsic optical sectioning and a reduced phototoxicity. On the other side, Two-Photon Excitation (2PE), thanks to the use of IR wavelengths, has become an invaluable tool to improve i…
Optical Characterization Method for Tilted or Decentered Intraocular Lenses
2016
Purpose To test and validate a method for measuring the optical quality and optical power of monofocal intraocular lenses (IOLs) in the presence of a tilt or a decentration as well as its optical power. Methods The experimental system consists of an artificial eye (wet-cell) and a commercial aberrometer with a Shack-Hartmann sensor. Optical image quality parameters such as point spread function (PSF), modulation transfer function (MTF), and the simulations of the retinal image of an extended object are computed from the in vitro wavefront data of an IOL. Repeatability and reproducibility of the system are tested. Mathematical and ray-tracing simulations are used to ascertain the precision a…
The ATHENA X-ray Integral Field Unit (X-IFU)
2018
Event: SPIE Astronomical Telescopes + Instrumentation, 2018, Austin, Texas, United States.