Search results for "synthesis"

showing 10 items of 2844 documents

A heterocyclic oxime from a fungus with anti-juvenile hormone activity

1998

Two fractions obtained after chromatography of dichloromethane extract of Penicillium brevicompactum culture medium showed anti-juvenile hormone activity. One was active when assayed in vivo against Oncopeltus fasciatus third-instar nymphs, whereas the other showed a strong in vitro inhibition of JH III biosynthesis on Locusta migratoria corpora allata. A subfraction of the latter, constituting 97% of this fraction, is a main component that possessed the juvenile hormone biosynthesis inhibitory activity. Chemical characterization of this compound shows a sesquiterpene-like structure, molecular mass of 278.16185 daltons, corresponding to an empirical formula of C15H22N2O3 named brevioxime. E…

Hormone activityMolecular massPhysiologyPenicillium brevicompactumGeneral MedicineBiologybiology.organism_classificationBiochemistryIn vitrochemistry.chemical_compoundBiosynthesischemistryBiochemistryIn vivoInsect ScienceJuvenile hormoneCorpus allatumArchives of Insect Biochemistry and Physiology
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Transcriptional and translational study of the Drosophila subobscura hsp83 gene in normal and heat-shock conditions

1993

In this paper we report a transcriptional and translational study of the hsp83 gene of Drosophila subobscura. This gene is located at the 18C region of the J chromosome. A monoclonal antibody raised against hsp83 was used for the immunological detection of this protein by Western blotting throughout the development of D. subobscura in control and heat-shock conditions. Our results indicate that puff 18C is not only heat-shock inducible but is also expressed during normal development and its level of expression increases at the end of the prepupa period. We detected hsp83 at normal temperatures, in particular developmental stages with the exception of the larval and the beginning of prepupa…

Hot TemperaturePolytene chromosomeTranscription GeneticGene ExpressionGenes InsectGeneral MedicineBiologybiology.organism_classificationMolecular biologyDrosophila subobscuraGene productTranscription (biology)Protein BiosynthesisHeat shock proteinDrosophilidaeGene expressionGeneticsAnimalsDrosophilaMolecular BiologyGeneHeat-Shock ProteinsBiotechnologyGenome
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Translational adaptation to heat stress is mediated by RNA 5‐methylcytosine in Caenorhabditis elegans

2021

Abstract Methylation of carbon‐5 of cytosines (m5C) is a post‐transcriptional nucleotide modification of RNA found in all kingdoms of life. While individual m5C‐methyltransferases have been studied, the impact of the global cytosine‐5 methylome on development, homeostasis and stress remains unknown. Here, using Caenorhabditis elegans, we generated the first organism devoid of m5C in RNA, demonstrating that this modification is non‐essential. Using this genetic tool, we determine the localisation and enzymatic specificity of m5C sites in the RNome in vivo. We find that NSUN‐4 acts as a dual rRNA and tRNA methyltransferase in C. elegans mitochondria. In agreement with leucine and proline bein…

Hot TemperatureProlineRibosomeGeneral Biochemistry Genetics and Molecular BiologyArticle03 medical and health sciencesNSUNCytosine0302 clinical medicineRNA modificationsLeucinem5CAnimalsRNA Processing Post-TranscriptionalCaenorhabditis elegansMolecular BiologytRNACaenorhabditis elegansprotein translation030304 developmental biologyGene Editing0303 health sciencesGeneral Immunology and MicrobiologybiologyGeneral NeuroscienceTRNA MethyltransferaseRNATranslation (biology)MethylationArticlesMethyltransferasesRibosomal RNAbiology.organism_classificationRNA BiologyAdaptation Physiological5‐methylcytosineCell biologyMitochondriatranslation efficiencyProtein BiosynthesisTransfer RNA5-MethylcytosineRNACRISPR-Cas SystemsRibosomes030217 neurology & neurosurgeryHeat-Shock ResponseThe EMBO Journal
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Polymethacrylate monoliths with immobilized poly-3-mercaptopropyl methylsiloxane film for high-coverage surface functionalization by thiol-ene click …

2014

In this work, new polythiol-functionalized macroporous monolithic polymethacrylate-polysiloxane composite materials are presented which can be useful substrates for highly efficient immobilization of (chiral) catalysts, chromatographic ligands, and other functional moieties by thiol-ene click reaction. Poly(glycidyl methacrylate-co-ethylene dimethacrylate) (poly(GMA-co-EDMA)) monoliths were coated with a poly-3-mercaptopropyl methylsiloxane (PMPMS) film and subsequently the polymer was covalently immobilized by formation of crosslinks via nucleophilic substitution reaction with pendent 2,3-epoxypropyl groups on the monolith surface. This monolith, though, showed similar levels of surface co…

Hot TemperatureSiloxanesSurface PropertiesEpoxideBiochemistryAnalytical Chemistrychemistry.chemical_compoundPolymethacrylic AcidsCapillary ElectrochromatographyPolymer chemistryMethylmethacrylatesSulfhydryl CompoundsMonolithEne reactionAminationCapillary electrochromatographygeographygeography.geographical_feature_categoryChromatographyOrganic ChemistryEnantioselective synthesisReproducibility of ResultsGeneral MedicinechemistryClick chemistrySurface modificationChromatography LiquidJournal of Chromatography A
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ID: 37

2015

During the early phase of human cytomegalovirus (HCMV) infection, the Interferon- γ -Inducible factor 16 (IFI16) behaves as a pattern recognition receptor (PRR) sensing viral DNA and triggering antiviral cytokine release. Later on, it restricts virus replication by down-regulating expression of viral genes committed to DNA synthesis including UL54 and UL44. These activities are modulated by viral proteins including pUL83, a tegument protein involved in viral evasion. Here, we demonstrate that pUL83 interacts with IFI16 relieving its inhibitory activity on UL54 gene transcription. We also establish that, starting from 48 h post-infection, IFI16 is stabilized and protected from degradation by…

Human cytomegalovirusDNA synthesisIFI16virusesImmunologyMutantHematologyBiologymedicine.diseaseBiochemistryMolecular biologyViral replicationInterferonGene expressionmedicineProteasome inhibitorImmunology and AllergyMolecular Biologymedicine.drugCytokine
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From medical data to simple virtual mock-up of scapulo-humeral joint

2008

The surgical operations of shoulder joint are guided by various principles: osteosynthesis in the case of fracture, osteotomy in order to correct a deformation or to modify the functioning of the joint, or implementation of articular prosthesis. At the end of the twentieth century, many innovations in the domains of biomechanics and orthopedic surgery have been performed. Nevertheless, theoretical and practical problems may appear during the operation (visual field of surgeon is very limited, quality and shape of the bone is variable depending on the patient). Biomechanical criteria of success are defined for each intervention. For example, the installation with success of prosthetic implan…

Humeral jointComputer engineering. Computer hardwaremedicine.medical_specialtyEngineeringHombroShoulderComputer sciencemedicine.medical_treatmentmedical imagingCirurgia virtualVirtual surgeryVirtual realityOsteotomyProsthesisEspatlla3D modelingTK7885-7895Physical medicine and rehabilitationImage processingMedical imagingmedicineProcesamiento de imágenesSimulationOsteosynthesisModelatge en 3Dbusiness.industryBiomechanicsCirugía virtualProcessament d'imatgesQA75.5-76.95image processingmedicine.anatomical_structureModelado en 3DImatges mèdiquesElectronic computers. Computer scienceImágenes médicasOrthopedic surgeryShoulder jointComputer Vision and Pattern RecognitionMedical imagingvirtual surgerybusinessSoftware
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Highly enantioselective synthesis of fluorinated gamma-amino alcohols through proline-catalyzed cross-Mannich reaction.

2005

A new, simple route for the synthesis of fluorinated beta-alkyl gamma-amino alcohols in optically pure form in only two steps and featuring proline catalysis from inexpensive and readily available starting materials is described. The applied strategy allows for the introduction of diversity into both the beta-fluoroalkyl and alpha-alkyl groups of these compounds. [reaction: see text]

Hydrocarbons FluorinatedProlineStereochemistryChemistryOrganic ChemistryEnantioselective synthesisStereoisomerismGeneral MedicineBiochemistryCombinatorial chemistryAmino AlcoholsCatalysisCatalysisMannich BasesOrganic chemistryProlinePhysical and Theoretical ChemistryMannich reactionOrganic letters
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New Cathepsin Inhibitors to Explore the Fluorophilic Properties of the S 2 Pocket of Cathepsin B: Design, Synthesis, and Biological Evaluation

2011

5 páginas, 1 figura, 2 tablas -- PAGS nros. 5256-5260

Hydrocarbons FluorinatedStereochemistryStereoisomerismhydrolasesstereoselectivityCatalysisCathepsin BCathepsin BNitrilesinhibitorsCombinatorial Chemistry TechniquesHumansMoleculefluorinated ligandsBiological evaluationCathepsinMolecular StructureCombinatorial Chemistry TechniquesligandsChemistryOrganic ChemistryStereoisomerismDipeptidesGeneral Chemistryfluorinated fluorinatedDesign synthesisDrug DesignpeptidesStereoselectivityChemistry – A European Journal
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An efficient synthesis of new fluorinated uracil derivatives.

2003

A series of potentially biologically active fluorinated uracil derivatives has been prepared in three steps from oxazolines and fluorinated nitriles with good chemical yields. Fustero Lardies, Santos, Santos.Fustero@uv.es ; Sanz Cervera, Juan Francisco, Juan.F.Sanz@uv.es ; Asensio Martinez, Amparo, Amparo.Asensio@uv.es

Hydrocarbons FluorinatedSynthesis ; Fluorinated uracil derivatives ; Oxazolines ; Biologically activeAntimetabolitesUNESCO::QUÍMICABiologically active:QUÍMICA::Química orgánica [UNESCO]UracilGeneral MedicineOxazolines:QUÍMICA [UNESCO]Combinatorial chemistryFluorinated uracil derivativesSynthesischemistry.chemical_compoundchemistryNitrilesUNESCO::QUÍMICA::Química orgánicaUracilOxazolesChemical communications (Cambridge, England)
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Dual Hydrogen Bond - Enamine Catalysis Enables a Direct Enantioselective Three-Component Domino Reaction

2011

A dual system, composed of an enantioselective enamine catalyst and a multiple-hydrogen-bond catalyst achieves the three-component enantioselective aldehyde—nitroalkene—aldehyde domino reaction using either two similar or two different aldehydes.

Hydrogen bond catalysisComponent (thermodynamics)Hydrogen bondChemistryEnantioselective synthesisGeneral MedicineGeneral ChemistryCatalysisCatalysisEnaminechemistry.chemical_compoundCascade reactionOrganocatalysisOrganic chemistryta116Angewandte Chemie Intermational Edition
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