showing 9 related works from this author
Impact of Oenococcus oeni esterase EstA7 on wine aromatic compounds
2015
Impact of Oenococcus oeni esterase EstA7 on wine aromatic compounds. 10. symposium international d'oenologie de Bordeaux (Oeno 2015).
Impact of lactic acid bacteria esterase activity on wine aromatic compounds
2014
Impact of lactic acid bacteria esterase activity on wine aromatic compounds. Macrowine 2014
Use of antisense RNA to modulate hsp gene expression in O. oeni
2015
Use of antisense RNA to modulate hsp gene expression in O. oeni. 6. Congress of European Microbiologists (FEMS 2015)
Characterization of the regulation of the CtsR Activity in O. oeni by using heterologous expression system
2014
Characterization of the regulation of the CtsR Activity in O. oeni by using heterologous expression system. 15. Forum des Jeunes Chercheurs (FJC)
Study of molecular mechanisms of stress response in Oenococcus oeni and implementation of tools for the functional exploration of enological genes
2015
O. oeni is responsible for wine malolactic fermentation. As any organism, O. oeni tries to adapt its physiology to environmental fluctuations by producing Hsp proteins encoded by the hsp genes. In O. oeni, CtsR is currently the only regulator of hsp genes. As an alternative to the lack of genetic tool, with the goal of understanding the mechanisms of O. oeni stress response, we developed a new expression vector, the pSIPSYN, to produce antisense RNA targeting of hsp18 mRNA. The synthesis of hsp18 asRNA leads to the decrease in the protein level of Lo18 and induced a loss of cultivability after heat or acid shock showing for the first time in vivo involvement of Lo18 in thermotolerance and a…
Etude du système AGR par caractérisation de mutants de Listeria monocytogènes
2012
EA MERS CT3 (vu DM) EJ3; Master; L’adaptation des bactéries à leur environnement fait intervenir différents mécanismes dont la communication cellulaire. Chez Listeria monocytogenes, le système agr est le seul système de communication cellulaire actuellement décrit. L’implication du système agr dans l’adaptation de L. monocytogenes aux changements de conditions environnementales a été étudiée au travers la caractérisation de deux mutants DG342M et DG343M. L’analyse des séquences et des transcrits par RTqPCR a montré que l’insertion du mariner avait un effet polaire sur l’expression du gène codant une phosphodiestérase (lmo2642), enzyme responsable de l’hydrolyse de l’AMPc. L’analyse a égalem…
The Antisense RNA Approach: a New Application for In Vivo Investigation of the Stress Response of Oenococcus oeni, a Wine-Associated Lactic Acid Bact…
2015
ABSTRACT Oenococcus oeni is a wine-associated lactic acid bacterium mostly responsible for malolactic fermentation in wine. In wine, O. oeni grows in an environment hostile to bacterial growth (low pH, low temperature, and ethanol) that induces stress response mechanisms. To survive, O. oeni is known to set up transitional stress response mechanisms through the synthesis of heat stress proteins (HSPs) encoded by the hsp genes, notably a unique small HSP named Lo18. Despite the availability of the genome sequence, characterization of O. oeni genes is limited, and little is known about the in vivo role of Lo18. Due to the lack of genetic tools for O. oeni , an efficient expression vector in O…
Oenococcus oeni : advances in molecular genetics
2017
International audience
Use of antisense RNA to modulate HSP gene expression in oenococcus oeni
2015
International audience